BMRB: 30945

Solution NMR structure of uperin 3.5 in SDS micelles

Authors

Rosengren, K.J., Armstrong, D.A.

Assembly

Uperin-3.5

Entity

1. Uperin-3.5 (polymer, Thiol state: not present), 18 monomers, 1779.155 Da Detail

GVGDLIRKAV SVIKNIVX

Formula weight

1779.155 Da

Source organism

Uperoleia mjobergii

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 89.3 %, Completeness (bb): 81.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.3 % (175 of 196)	99.0 % (99 of 100)	77.9 % (60 of 77)	84.2 % (16 of 19)
Backbone	81.4 % (83 of 102)	97.2 % (35 of 36)	65.3 % (32 of 49)	94.1 % (16 of 17)
Sidechain	98.2 % (107 of 109)	100.0 % (64 of 64)	100.0 % (43 of 43)	0.0 % (0 of 2)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. entity 1

GVGDLIRKAV SVIKNIVX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.25, Details 2 mg/mL uperin 3.5, 100 mM [U-100% 2H] SDS, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	uperin 3.5	natural abundance		2 mg/mL
2	SDS	[U-100% 2H]		100 mM

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Release date

2022-06-20

Citation

Secondary Structure Transitions for a Family of Amyloidogenic, Antimicrobial Uperin 3 Peptides in Contact with Sodium Dodecyl Sulfate
Prasad, A.K., Tiwari, C., Ray, S., Holden, S., Armstrong, D.A., Rosengren, K.J., Rodger, A., Panwar, A.S., Martin, L.L.
Chempluschem (2022), 87, e202100408-e202100408, PubMed 35032115 , DOI 10.1002/cplu.202100408 , Abstract

Related entities 1. Uperin-3.5, : 2 : 2 entities Detail

Experiments performed 4 experiments Detail