BMRBj - BMREntryMaster

Found 1 Document (0.78 seconds)

BMRB: 30956

7SEK

Solution structure of the zinc finger domain of murine MetAP1, complexed with ZNG N-terminal peptide

Authors

Edmonds, K.A., Jordan, M.R., Thalluri, K., Wu, H., Di Marchi, R., Giedroc, D.P.

Assembly

COBW domain-containing protein 1,Methionine aminopeptidase 1 (E.C.3.4.11.18)

Entity

1. COBW domain-containing protein 1,Methionine aminopeptidase 1 (E.C.3.4.11.18), entity 1 (polymer), 80 monomers, 8944.026 Da Detail

AEEEYAEDCP ELVPIETKNQ EMAAVETRVC ETDGCSSEAK LQCPTCIKLG IQGSYFCSQE CFKGSWATHK LLHKKAKDEK

2. COBW domain-containing protein 1,Methionine aminopeptidase 1 (E.C.3.4.11.18), entity ZN (non-polymer), 65.409 × 2 Da

Total weight

9074.845 Da

Max. entity weight

8944.026 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS30:SG	2:ZN1:ZN
2	na	sing	1:CYS35:SG	2:ZN1:ZN
3	na	sing	1:CYS57:SG	2:ZN1:ZN
4	na	sing	1:CYS61:SG	2:ZN1:ZN
5	na	sing	1:CYS43:SG	2:ZN1:ZN
6	na	sing	1:CYS46:SG	2:ZN1:ZN
7	na	sing	1:HIS69:NE2	2:ZN1:ZN
8	na	sing	1:HIS73:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.9 % (893 of 912)	98.1 % (468 of 477)	98.0 % (344 of 351)	96.4 % (81 of 84)
Backbone	98.5 % (467 of 474)	97.5 % (157 of 161)	99.6 % (235 of 236)	97.4 % (75 of 77)
Sidechain	97.7 % (502 of 514)	98.4 % (311 of 316)	96.9 % (185 of 191)	85.7 % (6 of 7)
Aromatic	89.3 % (50 of 56)	100.0 % (28 of 28)	77.8 % (21 of 27)	100.0 % (1 of 1)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. entity 1

AEEEYAEDCP ELVPIETKNQ EMAAVETRVC ETDGCSSEAK LQCPTCIKLG IQGSYFCSQE CFKGSWATHK LLHKKAKDEK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7 (±0.05), Details 2 mM [U-13C; U-15N] ZNG-Metap1 fusion, 150 mM sodium chloride, 10 mM sodium phosphate, 2 mM TCEP, 0.3 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7 (±0.05), Details 2 mM [U-13C; U-15N] ZNG-Metap1 fusion, 150 mM sodium chloride, 10 mM sodium phosphate, 2 mM TCEP, 0.3 mM DSS, 100% D2O

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7 (±0.05), Details 2 mM [U-10% 13C; U-100% 15N] ZNG-Metap1 fusion, 150 mM sodium chloride, 10 mM sodium phosphate, 2 mM TCEP, 0.3 mM DSS, 100% D2O

#	Name	Isotope labeling	Concentration
11	ZNG-Metap1 fusion	[U-10% 13C; U-100% 15N]	2 (±0.01) mM
12	sodium chloride	natural abundance	150 (±0.01) mM
13	sodium phosphate	natural abundance	10 (±0.01) mM
14	TCEP	natural abundance	2 (±0.01) mM
15	DSS	natural abundance	0.3 (±0.01) mM

Release date

2021-10-04

Citation

Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis
Weiss, A., Murdoch, C.C., Edmonds, K.A., Jordan, M.R., Monteith, A.J., Perera, Y.R., Rodriguez Nassif, A.M., Petoletti, A.M., Beavers, W.N., Munneke, M.J., Drury, S.L., Krystofiak, E.S., Thalluri, K., Wu, H., Kruse, A., DiMarchi, R.D., Caprioli, R.M., Spraggins, J.M., Chazin, W.J., Giedroc, D.P., Skaar, E.P.
Cell (2022), 185, 2148-2163, PubMed 35584702 , DOI 10.1016/j.cell.2022.04.011 , Abstract

Related entities 1. COBW domain-containing protein 1,Methionine aminopeptidase 1 (E.C.3.4.11.18), entity 1, : 7 entities Detail

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7 (±0.05), Details 2 mM [U-13C; U-15N] ZNG-Metap1 fusion, 150 mM sodium chloride, 10 mM sodium phosphate, 2 mM TCEP, 0.3 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

2 3D HNCACB

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

3 3D CBCA(CO)NH

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

4 3D HNCO

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

5 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

6 3D HN(CA)CO

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

8 3D HCCH-TOCSY

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7 (±0.05), Details 2 mM [U-13C; U-15N] ZNG-Metap1 fusion, 150 mM sodium chloride, 10 mM sodium phosphate, 2 mM TCEP, 0.3 mM DSS, 100% D2O

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

9 3D HCCH-TOCSY

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

10 3D C(CO)NH

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

11 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

12 3d Har(CC-TOCSY-CGCBCACO)NH

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

13 2D hbCBcgcdceHE

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

14 2D hbCBcgcdHD

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

15 2D 1H-13C HSQC aromatic

Instrument

Varian VNMRS - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

16 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

17 2D 1H-15N HSQC long-range

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
2	sodium chloride	natural abundance	150 (±0.01) mM
3	sodium phosphate	natural abundance	10 (±0.01) mM
4	TCEP	natural abundance	2 (±0.01) mM
5	DSS	natural abundance	0.3 (±0.01) mM

18 2D 1H-13C HSQC CT

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7 (±0.05), Details 2 mM [U-10% 13C; U-100% 15N] ZNG-Metap1 fusion, 150 mM sodium chloride, 10 mM sodium phosphate, 2 mM TCEP, 0.3 mM DSS, 100% D2O

#	Name	Isotope labeling	Concentration
11	ZNG-Metap1 fusion	[U-10% 13C; U-100% 15N]	2 (±0.01) mM
12	sodium chloride	natural abundance	150 (±0.01) mM
13	sodium phosphate	natural abundance	10 (±0.01) mM
14	TCEP	natural abundance	2 (±0.01) mM
15	DSS	natural abundance	0.3 (±0.01) mM

19 2D 1H-13C HSQC CT

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
11	ZNG-Metap1 fusion	[U-10% 13C; U-100% 15N]	2 (±0.01) mM
12	sodium chloride	natural abundance	150 (±0.01) mM
13	sodium phosphate	natural abundance	10 (±0.01) mM
14	TCEP	natural abundance	2 (±0.01) mM
15	DSS	natural abundance	0.3 (±0.01) mM

20 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

21 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE NEO - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
6	ZNG-Metap1 fusion	[U-13C; U-15N]	2 (±0.01) mM
7	sodium chloride	natural abundance	150 (±0.01) mM
8	sodium phosphate	natural abundance	10 (±0.01) mM
9	TCEP	natural abundance	2 (±0.01) mM
10	DSS	natural abundance	0.3 (±0.01) mM

nullKeywords COG0523, METAL BINDING PROTEIN, METAP1, Metallochaperone, zinc finger

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Found 1 Document (0.78 seconds)

BMRB: 30956

Sample #1

Sample #2

Sample #3

Related entities 1. COBW domain-containing protein 1,Methionine aminopeptidase 1 (E.C.3.4.11.18), entity 1, : 7 entities Detail

Experiments performed 21 experiments Detail

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