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BMRB: 30959

7SGT

Domain III (EDIII) of the POWV E glycoprotein

Authors

Harris, R., Cahill, S.M., Cowburn, D.

Assembly

Envelope protein E

Entity

1. Envelope protein E (polymer), 102 monomers, 11170.66 Da Detail

SNTYSMCDKT KFKWKRVPVD SGHDTVVMEV SYTGSDKPCR IPVRAVAHGV PTINVAMLIT PNPTIETSGG GFIEMQLPPG DNIIYVGDLS QQWFQKGSTI GR

Formula weight

11170.66 Da

Source organism

Tick-borne powassan virus (strain lb)

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 11.8 %, Completeness: 8.9 %, Completeness (bb): 10.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	8.9 % (104 of 1167)	8.6 % (52 of 603)	8.8 % (40 of 456)	11.1 % (12 of 108)
Backbone	10.2 % (61 of 596)	11.2 % (23 of 206)	9.1 % (27 of 296)	11.7 % (11 of 94)
Sidechain	7.5 % (50 of 663)	7.3 % (29 of 397)	7.9 % (20 of 252)	7.1 % (1 of 14)
Aromatic	14.0 % (12 of 86)	14.0 % (6 of 43)	12.2 % (5 of 41)	50.0 % (1 of 2)
Methyl	7.4 % (8 of 108)	7.4 % (4 of 54)	7.4 % (4 of 54)

1. entity 1

SNTYSMCDKT KFKWKRVPVD SGHDTVVMEV SYTGSDKPCR IPVRAVAHGV PTINVAMLIT PNPTIETSGG GFIEMQLPPG DNIIYVGDLS QQWFQKGSTI GR

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 278 (±0.1) K, pH 6.2 (±.1), Details .5 mM [U-15N] domain III (EDIII) of the POWV E glycoprotein, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	.5 (±0.1) mM
2	sodium phosphate	none	20 (±0.01) mM
3	sodium chloride	none	50 (±0.1) mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 278 (±0.1) K, pH 6.2 (±.1), Details 1 mM [U-15N] domain III (EDIII) of the POWV E glycoprotein, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

Sample #3

Solvent system 100% D2O, Pressure 1 (±0) atm, Temperature 278 (±0.1) K, pH 6.2 (±.1), Details 1 mM [U-15N] domain III (EDIII) of the POWV E glycoprotein, 20 mM sodium phosphate, 50 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
7	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
8	sodium phosphate	none	20 (±0.01) mM
9	sodium chloride	none	50 (±0.1) mM

Release date

2021-10-12

Citation

A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice
Malonis, R.J., Georgiev, G.I., Haslwanter, D., VanBlargan, L.A., Fallon, G., Vergnolle, O., Cahill, S.M., Harris, R., Cowburn, D., Chandran, K., Diamond, M.S., Lai, J.R.
PLoS Pathog. (2022), 18, e1010573-e1010573, PubMed 35679349 , DOI 10.1371/journal.ppat.1010573 , Abstract

Related entities 1. Envelope protein E, : 1 : 1 : 176 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 600 MHz Einstein

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 278 (±0.1) K, pH 6.2 (±.1), Details .5 mM [U-15N] domain III (EDIII) of the POWV E glycoprotein, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	.5 (±0.1) mM
2	sodium phosphate	none	20 (±0.01) mM
3	sodium chloride	none	50 (±0.1) mM

2 3D 1H-15N NOESY

Instrument

Bruker AVANCE III HD - 600 MHz Einstein

Sample

#	Name	Isotope labeling	Concentration
1	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	.5 (±0.1) mM
2	sodium phosphate	none	20 (±0.01) mM
3	sodium chloride	none	50 (±0.1) mM

3 3D 1H-15N TOCSY

Instrument

Bruker AVANCE III HD - 600 MHz Einstein

Sample

#	Name	Isotope labeling	Concentration
1	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	.5 (±0.1) mM
2	sodium phosphate	none	20 (±0.01) mM
3	sodium chloride	none	50 (±0.1) mM

4 HNHA

Instrument

Bruker AVANCE III HD - 600 MHz Einstein

Sample

#	Name	Isotope labeling	Concentration
1	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	.5 (±0.1) mM
2	sodium phosphate	none	20 (±0.01) mM
3	sodium chloride	none	50 (±0.1) mM

5 2D 1H-15N HSQC

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 278 (±0.1) K, pH 6.2 (±.1), Details 1 mM [U-15N] domain III (EDIII) of the POWV E glycoprotein, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

6 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 (±0) atm, Temperature 278 (±0.1) K, pH 6.2 (±.1), Details 1 mM [U-15N] domain III (EDIII) of the POWV E glycoprotein, 20 mM sodium phosphate, 50 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
7	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
8	sodium phosphate	none	20 (±0.01) mM
9	sodium chloride	none	50 (±0.1) mM

7 3D 1H-13C NOESY

Instrument

Bruker AVANCE III HD - 700 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
7	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
8	sodium phosphate	none	20 (±0.01) mM
9	sodium chloride	none	50 (±0.1) mM

8 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
7	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
8	sodium phosphate	none	20 (±0.01) mM
9	sodium chloride	none	50 (±0.1) mM

9 3D HNCO

Instrument

Bruker AVANCE III HD - 700 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

10 3D HN(CO)CA

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

11 3D HNCA

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

12 HN(CA)CB

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

13 3D HN(COCA)CB

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

14 HN(CO)NH TOCSY

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
4	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
5	sodium phosphate	none	20 (±0.01) mM
6	sodium chloride	none	50 (±0.1) mM

15 HC(CO)NH TOCSY

Instrument

Bruker AVANCE DMX - 800 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
7	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
8	sodium phosphate	none	20 (±0.01) mM
9	sodium chloride	none	50 (±0.1) mM

16 13C HSQC aro

Instrument

Bruker AVANCE III HD - 700 MHz NYSBC

Sample

#	Name	Isotope labeling	Concentration
7	domain III (EDIII) of the POWV E glycoprotein	[U-15N]	1 (±0.1) mM
8	sodium phosphate	none	20 (±0.01) mM
9	sodium chloride	none	50 (±0.1) mM

nullKeywords Powassan virus, VIRAL PROTEIN, flavivirus, monoclonal antibody, nanoparticle immunogen

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Found 1 Document (0.6 seconds)

BMRB: 30959

Sample #1

Sample #2

Sample #3

Related entities 1. Envelope protein E, : 1 : 1 : 176 entities Detail

Experiments performed 16 experiments Detail

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