BMRB: 30976

The peptide Lt-MAP4 is an analog derived from the Ltc-3a. The primary sequence of the parental peptide was used as template for rational design, using the amino acid residues for modification of charge and hydrophobicity.

Authors

Freitas, C.D.P., Moraes, L.F.R.N., Migliolo, L., Liao, L.M.

Assembly

Lt-MAP4 (LKKLWRFLKKL)

Entity

1. Lt-MAP4 (LKKLWRFLKKL) (polymer, Thiol state: not present), 11 monomers, 1472.902 Da Detail

LKKLWRFLKK L

Formula weight

1472.902 Da

Source organism

Lachesana tarabaevi

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 72.1 %, Completeness (bb): 72.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	72.1 % (119 of 165)	84.4 % (81 of 96)	55.1 % (38 of 69)
Backbone	72.7 % (40 of 55)	95.5 % (21 of 22)	57.6 % (19 of 33)
Sidechain	71.9 % (87 of 121)	81.1 % (60 of 74)	57.4 % (27 of 47)
Aromatic	52.4 % (11 of 21)	54.5 % (6 of 11)	50.0 % (5 of 10)
Methyl	75.0 % (12 of 16)	75.0 % (6 of 8)	75.0 % (6 of 8)

1. entity 1

LKKLWRFLKK L

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 4.46, Details 1 mM Latarasin-L4, 100 mM [U-98% 2H] SDS-d25, 0.035 % [U-98% 2H] DSS-d6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Latarasin-L4	natural abundance		1 mM
2	SDS-d25	[U-98% 2H]		100 mM
3	DSS-d6	[U-98% 2H]		0.035 %

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Release date

2022-01-13

Citation

Two-dimensional NMR structural study of latarasin analogue Lt-MAP4 multifunctional synthetic peptide
Freitas, C.D.P., Liao, L.M., Migliolo, L., Moraes, L.F.R.N.

Experiments performed 3 experiments Detail