BMRBj - BMREntryMaster

Found 1 Document (0.458 seconds)

BMRB: 30987

7TV5

Disulfide-rich venom peptide lasiocepsin: P20A mutant

Authors

Cabalteja, C.C., Harmon, T.H., Rao, S.R., Horne, W.S.

Assembly

Lasiocepsin

Entity

1. Lasiocepsin (polymer, Thiol state: all disulfide bound), 28 monomers, 2869.734 Da Detail

GLPRKILCAI AKKKGKCKGA LKLVCKCX

Formula weight

2869.734 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS25:SG
2	disulfide	sing	1:CYS17:SG	1:CYS27:SG

Source organism

Lasioglossum laticeps

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 99.4 %, Completeness (bb): 98.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	99.4 % (176 of 177)	99.4 % (176 of 177)
Backbone	98.2 % (55 of 56)	98.2 % (55 of 56)
Sidechain	100.0 % (121 of 121)	100.0 % (121 of 121)
Methyl	100.0 % (17 of 17)	100.0 % (17 of 17)

1. entity 1

GLPRKILCAI AKKKGKCKGA LKLVCKCX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 4, Details 1.2 mM Disulfide-rich venom peptide lasiocepsin: P20A mutant, 0.18 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Disulfide-rich venom peptide lasiocepsin: P20A mutant	natural abundance		1.2 mM
2	DSS	natural abundance		0.18 mM

Release date

2022-02-27

Citation

Heterogeneous-Backbone Proteomimetic Analogues of Lasiocepsin, a Disulfide-Rich Antimicrobial Peptide with a Compact Tertiary Fold
Cabalteja, C.C., Lin, Q., Harmon, T.H., Rao, S.R., Di, Y.P., Horne, W.S.
ACS Chem. Biol. (2022), 17, 987-997, PubMed 35290019 , DOI 10.1021/acschembio.2c00138 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 30988 released on 2022-02-27
    Title Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: native loop
  BMRB: 30989 released on 2022-02-27
    Title Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: beta-3-Lys modified loop
  BMRB: 30990 released on 2022-02-27
    Title Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: D-Ala modified loop
  BMRB: 30932 released on 2021-07-08
    Title Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin

Related entities 1. Lasiocepsin, : 1 : 1 : 7 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:8:CYS:SG	1:25:CYS:SG	unknown	unknown	n/a
1:17:CYS:SG	1:27:CYS:SG	unknown	unknown	n/a

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
1	28	NH2	AMINO GROUP	Assigned chemical shifts

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	212		100.0 (chain: 1, length: 28)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	179	178	99.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	56	55	98.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	123	123	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	17	100.0

Keywords ANTIBIOTIC, foldamer, peptidomimetic, proteomimetic

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30987_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

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Found 1 Document (0.458 seconds)

BMRB: 30987

Sample

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. Lasiocepsin, : 1 : 1 : 7 entities Detail

Experiments performed 4 experiments Detail

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Chemical shift validation 3 contents Detail