BMRB: 30990

Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: D-Ala modified loop

Authors

Cabalteja, C.C., Harmon, T.H., Rao, S.R., Horne, W.S.

Assembly

Lasiocepsin heterogeneous-backbone proteomimetic analogue

Entity

1. Lasiocepsin heterogeneous-backbone proteomimetic analogue (polymer, Thiol state: all disulfide bound), 28 monomers, 2933.819 Da Detail

GLXRKXLCAX AKXKXKCKXA XKLXCKCX

Formula weight

2933.819 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS25:SG
2	disulfide	sing	1:CYS17:SG	1:CYS27:SG

Source organism

Lasioglossum laticeps

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 67.9 %, Completeness: 97.7 %, Completeness (bb): 97.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.7 % (125 of 128)	97.7 % (125 of 128)
Backbone	97.4 % (38 of 39)	97.4 % (38 of 39)
Sidechain	97.8 % (87 of 89)	97.8 % (87 of 89)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. entity 1

GLXRKXLCAX AKXKXKCKXA XKLXCKCX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 4, Details 1.6 mM Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: D-Ala modified loop, 0.25 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Heterogeneous-backbone proteomimetic analogue of the disulfide-rich venom peptide lasiocepsin: D-Ala modified loop	natural abundance		1.6 mM
2	DSS	natural abundance		0.25 mM

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Release date

2022-02-27

Citation

Heterogeneous-Backbone Proteomimetic Analogues of Lasiocepsin, a Disulfide-Rich Antimicrobial Peptide with a Compact Tertiary Fold
Cabalteja, C.C., Lin, Q., Harmon, T.H., Rao, S.R., Di, Y.P., Horne, W.S.
ACS Chem. Biol. (2022), 17, 987-997, PubMed 35290019 , DOI 10.1021/acschembio.2c00138 , Abstract

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. Lasiocepsin heterogeneous-backbone proteomimetic analogue, : 1 : 2 : 1 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail