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Found 1 Document (5.12 seconds)

BMRB: 30995

7U37

Solution NMR structure of Vibrio cholerae ferrous iron transport protein C (FeoC)

Authors

Brown, J.B., Lee, M.A., Smith, A.T.

Assembly

Ferrous iron transport protein C

Entity

1. Ferrous iron transport protein C (polymer, Thiol state: not present), 77 monomers, 8580.850 Da Detail

GMILNELKAA IESKNGATRQ ELARRFALSE DGIDAMLAVW IKKGVLSRQQ YINAEDEVVR VRYVMNQVGS LAVNVTM

Formula weight

8580.85 Da

Source organism

Vibrio cholerae O1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.8 %, Completeness (bb): 91.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.8 % (689 of 897)	68.6 % (317 of 462)	83.9 % (287 of 342)	91.4 % (85 of 93)
Backbone	91.8 % (424 of 462)	84.3 % (134 of 159)	94.7 % (214 of 226)	98.7 % (76 of 77)
Sidechain	66.1 % (335 of 507)	60.7 % (184 of 303)	75.5 % (142 of 188)	56.3 % (9 of 16)
Aromatic	63.2 % (24 of 38)	68.4 % (13 of 19)	55.6 % (10 of 18)	100.0 % (1 of 1)
Methyl	68.9 % (73 of 106)	69.8 % (37 of 53)	67.9 % (36 of 53)

1. entity 1

GMILNELKAA IESKNGATRQ ELARRFALSE DGIDAMLAVW IKKGVLSRQQ YINAEDEVVR VRYVMNQVGS LAVNVTM

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 1 g/L [U-99% 15N] ammonium chloride, 300 uM [U-15N] Vibrio cholerae FeoC, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	5 mM
3	ammonium chloride	[U-99% 15N]	1 g/L
4	Vibrio cholerae FeoC	[U-15N]	300 uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 1 g/L [U-15N] ammonium chloride, 4 g/L [U-100% 13C] glucose, 300 uM [U-13C; U-15N] Vibrio cholerae FeoC, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	50 mM
6	sodium chloride	natural abundance	5 mM
7	ammonium chloride	[U-15N]	1 g/L
8	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
9	glucose	[U-100% 13C]	4 g/L

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 4 g/L [U-100% 13C] glucose, 300 uM [U-13C; U-15N] Vibrio cholerae FeoC, 100% D2O

#	Name	Isotope labeling	Concentration
10	sodium phosphate	natural abundance	50 mM
11	sodium chloride	natural abundance	5 mM
12	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
13	glucose	[U-100% 13C]	4 g/L

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 300 uM [U-13C; U-15N] Vibrio cholerae FeoC, 100% D2O

#	Name	Isotope labeling	Concentration
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	5 mM
16	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM

Release date

2022-03-01

Citation

The structure of Vibrio cholerae FeoC reveals conservation of the helix-turn-helix motif but not the cluster-binding domain
Brown, J.B., Lee, M.A., Smith, A.T.
J. Biol. Inorg. Chem. (2022), 27, 485-495, PubMed 35796835 , DOI 10.1007/s00775-022-01945-4 , Abstract

Related entities 1. Ferrous iron transport protein C, : 1 : 9 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 1 g/L [U-99% 15N] ammonium chloride, 300 uM [U-15N] Vibrio cholerae FeoC, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	5 mM
3	ammonium chloride	[U-99% 15N]	1 g/L
4	Vibrio cholerae FeoC	[U-15N]	300 uM

2 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	5 mM
3	ammonium chloride	[U-99% 15N]	1 g/L
4	Vibrio cholerae FeoC	[U-15N]	300 uM

3 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 1 g/L [U-15N] ammonium chloride, 4 g/L [U-100% 13C] glucose, 300 uM [U-13C; U-15N] Vibrio cholerae FeoC, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	50 mM
6	sodium chloride	natural abundance	5 mM
7	ammonium chloride	[U-15N]	1 g/L
8	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
9	glucose	[U-100% 13C]	4 g/L

4 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	50 mM
6	sodium chloride	natural abundance	5 mM
7	ammonium chloride	[U-15N]	1 g/L
8	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
9	glucose	[U-100% 13C]	4 g/L

5 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	50 mM
6	sodium chloride	natural abundance	5 mM
7	ammonium chloride	[U-15N]	1 g/L
8	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
9	glucose	[U-100% 13C]	4 g/L

6 3D HN(CA)CO

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	50 mM
6	sodium chloride	natural abundance	5 mM
7	ammonium chloride	[U-15N]	1 g/L
8	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
9	glucose	[U-100% 13C]	4 g/L

7 4D HSQC-NOESY-HMQC

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	sodium phosphate	natural abundance	50 mM
6	sodium chloride	natural abundance	5 mM
7	ammonium chloride	[U-15N]	1 g/L
8	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
9	glucose	[U-100% 13C]	4 g/L

8 4D HMQC-NOESY-HMQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 4 g/L [U-100% 13C] glucose, 300 uM [U-13C; U-15N] Vibrio cholerae FeoC, 100% D2O

#	Name	Isotope labeling	Concentration
10	sodium phosphate	natural abundance	50 mM
11	sodium chloride	natural abundance	5 mM
12	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM
13	glucose	[U-100% 13C]	4 g/L

9 2D 1H-1H NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 50 mM sodium phosphate, 5 mM sodium chloride, 300 uM [U-13C; U-15N] Vibrio cholerae FeoC, 100% D2O

#	Name	Isotope labeling	Concentration
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	5 mM
16	Vibrio cholerae FeoC	[U-13C; U-15N]	300 uM

nullKeywords CYTOSOLIC PROTEIN, Feo, FeoC, ferrous iron transport

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Found 1 Document (5.12 seconds)

BMRB: 30995

Sample #1

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Sample #4

Related entities 1. Ferrous iron transport protein C, : 1 : 9 entities Detail

Experiments performed 9 experiments Detail

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