NMR structure of the cNTnC-cTnI chimera bound to W8
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKFKRPTLRR VRISADAMMQ ALLGARAKGH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.5 % (1111 of 1452) | 78.6 % (594 of 756) | 72.0 % (403 of 560) | 83.8 % (114 of 136) |
Backbone | 76.5 % (569 of 744) | 82.8 % (212 of 256) | 66.4 % (243 of 366) | 93.4 % (114 of 122) |
Sidechain | 78.6 % (648 of 824) | 76.4 % (382 of 500) | 85.8 % (266 of 310) | 0.0 % (0 of 14) |
Aromatic | 38.0 % (35 of 92) | 39.1 % (18 of 46) | 37.0 % (17 of 46) | |
Methyl | 89.8 % (106 of 118) | 84.7 % (50 of 59) | 94.9 % (56 of 59) |
1. entity 1
MDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGSISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRSMKDDSK GKFKRPTLRR VRISADAMMQ ALLGARAKGH HHHHHSolvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7 (±0.1), Details 0.4 mM [U-100% 13C; U-100% 15N] cChimera_protein, 100 mM potassium chloride, 10 mM imidazole, 10 mM calcium chloride, 0.25 mM [U-99% 2H] DSS, 2.5 mM W8, 5 % [U-99% 2H] DMSO, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cChimera_protein | [U-100% 13C; U-100% 15N] | 0.4 mM | |
2 | potassium chloride | natural abundance | 100 mM | |
3 | imidazole | natural abundance | 10 mM | |
4 | calcium chloride | natural abundance | 10 mM | |
5 | DSS | [U-99% 2H] | 0.25 mM | |
6 | W8 | natural abundance | 2.5 mM | |
7 | DMSO | [U-99% 2H] | 5 % |