BMRB: 31030

Site 2 insulin receptor binding peptide IM459N21

Authors

Lawrence, M.C., Hu, H., Martinez, F.J., Espinosa, J.F.

Assembly

Site 2 binding peptide IM459N21

Entity

1. Site 2 binding peptide IM459N21 (polymer, Thiol state: all disulfide bound), 22 monomers, 2627.062 Da Detail

XSLEQEWXKI ECEVYGKCPP KX

Formula weight

2627.062 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS12:SG	1:CYS18:SG

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.4 %, Completeness: 91.8 %, Completeness (bb): 80.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	91.8 % (201 of 219)	100.0 % (128 of 128)	80.2 % (73 of 91)
Backbone	80.6 % (75 of 93)	100.0 % (37 of 37)	67.9 % (38 of 56)
Sidechain	100.0 % (144 of 144)	100.0 % (91 of 91)	100.0 % (53 of 53)
Aromatic	100.0 % (19 of 19)	100.0 % (10 of 10)	100.0 % (9 of 9)
Methyl	100.0 % (12 of 12)	100.0 % (6 of 6)	100.0 % (6 of 6)

1. entity 1

XSLEQEWXKI ECEVYGKCPP KX

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 20 mM [U-2H] Tris, 100 mM sodium chloride, 0.75 mM peptide, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	[U-2H]		20 mM
2	sodium chloride	natural abundance		100 mM
3	peptide	natural abundance		0.75 mM

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Release date

2022-06-30

Citation

Activation of the human insulin receptor by non-insulin-related peptides
Lawrence, M.C., Hu, H., Martinez, F.J., Espinosa, J.F.
Nat. Commun. (2022), 13, 5695-5695, PubMed 36171189 , DOI 10.1038/s41467-022-33315-8 , Abstract

Related entities 1. Site 2 binding peptide IM459N21, : 1 : 1 : 3 entities Detail

Experiments performed 5 experiments Detail