BMRB: 31031

NMR Structure of Streptococcal Protein GB1 Backbone Modified Variant: beta-ACPC24, beta-3-Lys28, beta-3-Lys31, beta-ACPC35

Authors

Rao, S.R., Reinert, Z.E.

Assembly

Immunoglobulin G-binding protein G

Entity

1. Immunoglobulin G-binding protein G (polymer, Thiol state: not present), 57 monomers, 6242.789 Da Detail

DTYKLILNGK TLKGETTTEA VDAXTAEXVF XQYAXDNGVD GEWTYDDATK TFTVTEX

Formula weight

6242.789 Da

Source organism

Streptococcus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 98.6 %, Completeness (bb): 97.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.6 % (281 of 285)	98.6 % (281 of 285)
Backbone	97.2 % (105 of 108)	97.2 % (105 of 108)
Sidechain	99.4 % (176 of 177)	99.4 % (176 of 177)
Aromatic	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	100.0 % (32 of 32)	100.0 % (32 of 32)

1. entity 1

DTYKLILNGK TLKGETTTEA VDAXTAEXVF XQYAXDNGVD GEWTYDDATK TFTVTEX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.65 mM Streptococcal Protein GB1 Backbone Modified Variant: beta-ACPC24, beta-3-Lys28, beta-3-Lys31, beta-ACPC35, 20 mM sodium phosphate, 0.1 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin G-binding protein G	natural abundance		0.65 mM
2	sodium phosphate	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM

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Release date

2022-07-24

Related entities 1. Immunoglobulin G-binding protein G, : 1 : 72 : 32 entities Detail

Interaction partners 1. Immunoglobulin G-binding protein G, : 1 interactors Detail

Experiments performed 3 experiments Detail