BMRB: 31055

NMR solution structure of lambda-MeuKTx-1

Authors

Harvey, P.J., Craik, D.J.

Assembly

Neurotoxin lambda-MeuTx

Entity

1. Neurotoxin lambda-MeuTx (polymer, Thiol state: all disulfide bound), 38 monomers, 4129.625 Da Detail

GCNRLNKKCN SDADCCRYGE RCISTGVNYY CRPDVGPX

Formula weight

4129.625 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS16:SG
2	disulfide	sing	1:CYS9:SG	1:CYS22:SG
3	disulfide	sing	1:CYS15:SG	1:CYS31:SG

Source organism

Mesobuthus eupeus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 93.8 %, Completeness (bb): 98.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.8 % (198 of 211)	93.8 % (198 of 211)
Backbone	98.7 % (75 of 76)	98.7 % (75 of 76)
Sidechain	91.1 % (123 of 135)	91.1 % (123 of 135)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	100.0 % (10 of 10)	100.0 % (10 of 10)

1. entity 1

GCNRLNKKCN SDADCCRYGE RCISTGVNYY CRPDVGPX

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 3.0, Details 1.0 mM peptide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	peptide	natural abundance		1.0 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 3.0, Details 1.0 mM peptide, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	peptide	natural abundance		1.0 mM

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Release date

2022-11-16

Citation

Functional evolution of scorpion venom peptides with an inhibitor cystine knot fold
Gao, B., Harvey, P.J., Craik, D.J., Ronjat, M., De Waard, M., Zhu, S.
Biosci. Rep. (2013), 33, e00047-e00047, PubMed 23721518 , DOI 10.1042/BSR20130052 , Abstract

Related entities 1. Neurotoxin lambda-MeuTx, : 1 : 2 : 6 entities Detail

Experiments performed 7 experiments Detail