BMRB: 31061

Structure of pre-miR-31 reveals an active role in Dicer processing

Authors

Kotar, A., Ma, S., Keane, S.

Assembly

microRNA 31

Entity

1. microRNA 31 (polymer, Thiol state: not present), 71 monomers, 22935.56 Da Detail

GGAGAGGAGG CAAGAUGCUG GCAUAGCUGU UGAACUGGGA ACCUGCUAUG CCAACAUAUU GCCAUCUUUC C

Formula weight

22935.56 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 329, ¹³C: 54 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	G	H1'	H	5.9301	0.0000	1
2	1	1	G	H2'	H	4.9247	0.0000	1
3	1	1	G	H3'	H	4.6637	0.0000	1
4	1	1	G	H8	H	8.2665	0.0000	1
5	1	1	G	C8	C	140.3781	0.0000	1
6	1	2	G	H1'	H	5.8167	0.0000	1
7	1	2	G	H2'	H	4.7505	0.0000	1
8	1	2	G	H8	H	7.6369	0.0000	1
9	1	2	G	C8	C	136.9422	0.0000	1
10	1	3	A	H1'	H	6.1657	0.0000	1
11	1	3	A	H2	H	7.5105	0.0000	1
12	1	3	A	H2'	H	4.7564	0.0000	1
13	1	3	A	H3'	H	4.3147	0.0000	1
14	1	3	A	H8	H	7.9066	0.0000	1
15	1	3	A	C2	C	153.9109	0.0000	1
16	1	3	A	C8	C	140.9508	0.0000	1
17	1	4	G	H1'	H	5.6700	0.0000	1
18	1	4	G	H2'	H	4.5719	0.0000	1
19	1	4	G	H3'	H	4.3347	0.0000	1
20	1	4	G	H8	H	6.9721	0.0000	1
21	1	4	G	C8	C	137.2542	0.0000	1
22	1	5	A	H1'	H	6.0387	0.0000	1
23	1	5	A	H2	H	7.7233	0.0000	1
24	1	5	A	H2'	H	4.7865	0.0000	1
25	1	5	A	H3'	H	4.5783	0.0000	1
26	1	5	A	H8	H	7.7431	0.0000	1
27	1	5	A	C2	C	154.3963	0.0000	1
28	1	5	A	C8	C	139.9971	0.0000	1
29	1	6	G	H1'	H	5.7386	0.0000	1
30	1	6	G	H2'	H	4.5768	0.0000	1
31	1	6	G	H3'	H	4.7724	0.0000	1
32	1	6	G	H8	H	7.1342	0.0000	1
33	1	6	G	C8	C	136.6142	0.0000	1
34	1	7	G	H1'	H	5.8170	0.0000	1
35	1	7	G	H2'	H	4.5964	0.0000	1
36	1	7	G	H3'	H	4.6357	0.0000	1
37	1	7	G	H8	H	7.2350	0.0000	1
38	1	7	G	C8	C	137.7059	0.0000	1
39	1	8	A	H1'	H	6.1311	0.0000	1
40	1	8	A	H2	H	8.1440	0.0000	1
41	1	8	A	H2'	H	4.5831	0.0000	1
42	1	8	A	H3'	H	4.7406	0.0000	1
43	1	8	A	H8	H	7.9618	0.0000	1
44	1	8	A	C2	C	155.9452	0.0000	1
45	1	8	A	C8	C	139.8891	0.0000	1
46	1	9	G	H1'	H	5.6069	0.0000	1
47	1	9	G	H2'	H	4.5798	0.0000	1
48	1	9	G	H3'	H	4.3484	0.0000	1
49	1	9	G	H8	H	6.9889	0.0000	1
50	1	9	G	C8	C	137.0874	0.0000	1
51	1	10	G	H1'	H	5.8419	0.0000	1
52	1	10	G	H2'	H	4.6402	0.0000	1
53	1	10	G	H3'	H	4.6135	0.0000	1
54	1	10	G	H8	H	7.4171	0.0000	1
55	1	10	G	C8	C	136.9221	0.0000	1
56	1	11	C	H1'	H	5.5443	0.0000	1
57	1	11	C	H2'	H	4.5967	0.0000	1
58	1	11	C	H3'	H	4.6416	0.0000	1
59	1	11	C	H5	H	5.2494	0.0000	1
60	1	11	C	H6	H	7.6399	0.0000	1
61	1	12	A	H1'	H	5.9240	0.0000	1
62	1	12	A	H2	H	6.7454	0.0000	1
63	1	12	A	H2'	H	4.6408	0.0000	1
64	1	12	A	H3'	H	4.5644	0.0000	1
65	1	12	A	H8	H	7.9698	0.0000	1
66	1	12	A	C2	C	152.6281	0.0000	1
67	1	12	A	C8	C	140.2379	0.0000	1
68	1	13	A	H1'	H	5.8210	0.0000	1
69	1	13	A	H2	H	7.5083	0.0000	1
70	1	13	A	H2'	H	4.5644	0.0000	1
71	1	13	A	H3'	H	4.5775	0.0000	1
72	1	13	A	H8	H	7.7298	0.0000	1
73	1	13	A	C2	C	154.1683	0.0000	1
74	1	13	A	C8	C	139.3964	0.0000	1
75	1	14	G	H1'	H	5.8107	0.0000	1
76	1	14	G	H2'	H	4.5264	0.0000	1
77	1	14	G	H3'	H	4.5397	0.0000	1
78	1	14	G	H8	H	7.8116	0.0000	1
79	1	15	A	H1'	H	5.9787	0.0000	1
80	1	15	A	H2	H	7.3367	0.0000	1
81	1	15	A	H2'	H	4.5492	0.0000	1
82	1	15	A	H8	H	7.9165	0.0000	1
83	1	15	A	C2	C	154.1648	0.0000	1
84	1	15	A	C8	C	140.7966	0.0000	1
85	1	16	U	H1'	H	5.4283	0.0000	1
86	1	16	U	H2'	H	4.5700	0.0000	1
87	1	16	U	H3'	H	4.5372	0.0000	1
88	1	16	U	H5	H	5.0307	0.0000	1
89	1	16	U	H6	H	7.5682	0.0000	1
90	1	17	G	H1'	H	5.7722	0.0000	1
91	1	17	G	H2'	H	4.5398	0.0000	1
92	1	17	G	H8	H	7.6393	0.0000	1
93	1	17	G	C8	C	137.6954	0.0000	1
94	1	18	C	H2'	H	4.6461	0.0000	1
95	1	18	C	H3'	H	4.5608	0.0000	1
96	1	18	C	H5	H	5.5209	0.0000	1
97	1	18	C	H6	H	7.8709	0.0000	1
98	1	19	U	H1'	H	5.7584	0.0000	1
99	1	19	U	H2'	H	4.5773	0.0000	1
100	1	19	U	H3'	H	4.5289	0.0000	1
101	1	19	U	H5	H	5.6834	0.0000	1
102	1	19	U	H6	H	7.8327	0.0000	1
103	1	20	G	H1'	H	5.8187	0.0000	1
104	1	20	G	H2'	H	4.6360	0.0000	1
105	1	20	G	H3'	H	4.6202	0.0000	1
106	1	20	G	H8	H	7.8382	0.0000	1
107	1	20	G	C8	C	137.5641	0.0000	1
108	1	21	G	H1'	H	5.7341	0.0000	1
109	1	21	G	H2'	H	4.1851	0.0000	1
110	1	21	G	H3'	H	4.6873	0.0000	1
111	1	21	G	H8	H	7.3484	0.0000	1
112	1	21	G	C8	C	136.6564	0.0000	1
113	1	22	C	H1'	H	5.5565	0.0000	1
114	1	22	C	H2'	H	4.5917	0.0000	1
115	1	22	C	H3'	H	4.6926	0.0000	1
116	1	22	C	H5	H	5.2710	0.0000	1
117	1	22	C	H6	H	7.6563	0.0000	1
118	1	23	A	H1'	H	6.0169	0.0000	1
119	1	23	A	H2	H	7.3397	0.0000	1
120	1	23	A	H2'	H	4.6606	0.0000	1
121	1	23	A	H3'	H	4.6185	0.0000	1
122	1	23	A	H8	H	8.0731	0.0000	1
123	1	23	A	C2	C	153.7607	0.0000	1
124	1	23	A	C8	C	140.7649	0.0000	1
125	1	24	U	H1'	H	5.5241	0.0000	1
126	1	24	U	H2'	H	4.5917	0.0000	1
127	1	24	U	H3'	H	4.6269	0.0000	1
128	1	24	U	H5	H	5.1413	0.0000	1
129	1	24	U	H6	H	7.6989	0.0000	1
130	1	25	A	H1'	H	6.0518	0.0000	1
131	1	25	A	H2	H	6.8549	0.0000	1
132	1	25	A	H2'	H	4.6221	0.0000	1
133	1	25	A	H3'	H	4.6238	0.0000	1
134	1	25	A	H8	H	8.1430	0.0000	1
135	1	25	A	C2	C	153.2646	0.0000	1
136	1	25	A	C8	C	140.4582	0.0000	1
137	1	26	G	H1'	H	5.6890	0.0000	1
138	1	26	G	H2'	H	4.5301	0.0000	1
139	1	26	G	H3'	H	4.6030	0.0000	1
140	1	26	G	H8	H	7.3591	0.0000	1
141	1	26	G	C8	C	136.5396	0.0000	1
142	1	27	C	H1'	H	5.5606	0.0000	1
143	1	27	C	H3'	H	4.6092	0.0000	1
144	1	27	C	H5	H	5.2706	0.0000	1
145	1	27	C	H6	H	7.4762	0.0000	1
146	1	28	U	H1'	H	5.7172	0.0000	1
147	1	28	U	H2'	H	4.5606	0.0000	1
148	1	28	U	H3'	H	4.6147	0.0000	1
149	1	28	U	H5	H	5.6314	0.0000	1
150	1	28	U	H6	H	7.7476	0.0000	1
151	1	29	G	H1'	H	5.7980	0.0000	1
152	1	29	G	H2'	H	4.6183	0.0000	1
153	1	29	G	H3'	H	4.6636	0.0000	1
154	1	29	G	H8	H	7.9045	0.0000	1
155	1	29	G	C8	C	138.4464	0.0000	1
156	1	30	U	H1'	H	5.7935	0.0000	1
157	1	30	U	H2'	H	4.5448	0.0000	1
158	1	30	U	H3'	H	4.6602	0.0000	1
159	1	30	U	H5	H	5.3774	0.0000	1
160	1	30	U	H6	H	7.8177	0.0000	1
161	1	31	U	H1'	H	5.8280	0.0000	1
162	1	31	U	H2'	H	4.6360	0.0000	1
163	1	31	U	H3'	H	4.4830	0.0000	1
164	1	31	U	H5	H	5.7005	0.0000	1
165	1	31	U	H6	H	7.8694	0.0000	1
166	1	32	G	H1'	H	5.8181	0.0000	1
167	1	32	G	H2'	H	4.5715	0.0000	1
168	1	32	G	H3'	H	4.6237	0.0000	1
169	1	32	G	H8	H	7.8979	0.0000	1
170	1	32	G	C8	C	139.6355	0.0000	1
171	1	33	A	H1'	H	5.8786	0.0000	1
172	1	33	A	H2	H	7.8892	0.0000	1
173	1	33	A	H2'	H	4.7578	0.0000	1
174	1	33	A	H3'	H	4.8120	0.0000	1
175	1	33	A	H8	H	8.1978	0.0000	1
176	1	33	A	C2	C	155.4939	0.0000	1
177	1	33	A	C8	C	142.1314	0.0000	1
178	1	34	A	H1'	H	5.9267	0.0000	1
179	1	34	A	H2	H	8.1091	0.0000	1
180	1	34	A	H2'	H	4.5821	0.0000	1
181	1	34	A	H3'	H	4.8129	0.0000	1
182	1	34	A	H8	H	8.1922	0.0000	1
183	1	34	A	C2	C	156.0183	0.0000	1
184	1	34	A	C8	C	141.7073	0.0000	1
185	1	35	C	H1'	H	5.7602	0.0000	1
186	1	35	C	H2'	H	4.3382	0.0000	1
187	1	35	C	H3'	H	4.6459	0.0000	1
188	1	35	C	H5	H	5.7224	0.0000	1
189	1	35	C	H6	H	7.6388	0.0000	1
190	1	36	U	H1'	H	5.8665	0.0000	1
191	1	36	U	H2'	H	4.5860	0.0000	1
192	1	36	U	H3'	H	4.3341	0.0000	1
193	1	36	U	H5	H	5.8756	0.0000	1
194	1	36	U	H6	H	7.7834	0.0000	1
195	1	37	G	H1'	H	5.6946	0.0000	1
196	1	37	G	H2'	H	4.6035	0.0000	1
197	1	37	G	H3'	H	4.6491	0.0000	1
198	1	37	G	H8	H	7.9028	0.0000	1
199	1	37	G	C8	C	140.4803	0.0000	1
200	1	38	G	H1'	H	5.7056	0.0000	1
201	1	38	G	H2'	H	4.5805	0.0000	1
202	1	38	G	H3'	H	4.5476	0.0000	1
203	1	38	G	H8	H	7.8851	0.0000	1
204	1	38	G	C8	C	139.8869	0.0000	1
205	1	39	G	H1'	H	5.8106	0.0000	1
206	1	39	G	H2'	H	4.6457	0.0000	1
207	1	39	G	H3'	H	4.7002	0.0000	1
208	1	39	G	H8	H	8.0006	0.0000	1
209	1	39	G	C8	C	140.6255	0.0000	1
210	1	40	A	H1'	H	5.8744	0.0000	1
211	1	40	A	H2	H	7.5771	0.0000	1
212	1	40	A	H2'	H	4.5939	0.0000	1
213	1	40	A	H3'	H	4.6019	0.0000	1
214	1	40	A	H8	H	8.2647	0.0000	1
215	1	40	A	C2	C	154.4421	0.0000	1
216	1	40	A	C8	C	141.8667	0.0000	1
217	1	41	A	H1'	H	5.9201	0.0000	1
218	1	41	A	H2	H	7.9782	0.0000	1
219	1	41	A	H2'	H	4.5876	0.0000	1
220	1	41	A	H3'	H	4.6963	0.0000	1
221	1	41	A	H8	H	8.0601	0.0000	1
222	1	41	A	C2	C	155.1503	0.0000	1
223	1	41	A	C8	C	140.5703	0.0000	1
224	1	42	C	H1'	H	5.5499	0.0000	1
225	1	42	C	H2'	H	4.5182	0.0000	1
226	1	42	C	H3'	H	4.6036	0.0000	1
227	1	42	C	H5	H	5.3927	0.0000	1
228	1	42	C	H6	H	7.4578	0.0000	1
229	1	43	C	H1'	H	5.5191	0.0000	1
230	1	43	C	H2'	H	4.5813	0.0000	1
231	1	43	C	H5	H	5.7613	0.0000	1
232	1	43	C	H6	H	7.7396	0.0000	1
233	1	44	U	H1'	H	5.6932	0.0000	1
234	1	44	U	H2'	H	4.5852	0.0000	1
235	1	44	U	H3'	H	4.6247	0.0000	1
236	1	44	U	H5	H	5.6747	0.0000	1
237	1	44	U	H6	H	7.8454	0.0000	1
238	1	45	G	H1'	H	5.9025	0.0000	1
239	1	45	G	H2'	H	4.5896	0.0000	1
240	1	45	G	H3'	H	4.5901	0.0000	1
241	1	45	G	H8	H	7.9602	0.0000	1
242	1	45	G	C8	C	138.0834	0.0000	1
243	1	46	C	H1'	H	5.6707	0.0000	1
244	1	46	C	H2'	H	4.5871	0.0000	1
245	1	46	C	H3'	H	4.5478	0.0000	1
246	1	46	C	H5	H	5.3766	0.0000	1
247	1	46	C	H6	H	7.7318	0.0000	1
248	1	47	U	H1'	H	5.6328	0.0000	1
249	1	47	U	H2'	H	4.7029	0.0000	1
250	1	47	U	H3'	H	4.6261	0.0000	1
251	1	47	U	H5	H	5.5521	0.0000	1
252	1	47	U	H6	H	7.9934	0.0000	1
253	1	48	A	H1'	H	6.0743	0.0000	1
254	1	48	A	H2	H	7.0974	0.0000	1
255	1	48	A	H2'	H	4.6720	0.0000	1
256	1	48	A	H3'	H	4.6360	0.0000	1
257	1	48	A	H8	H	8.2772	0.0000	1
258	1	48	A	C2	C	153.7951	0.0000	1
259	1	48	A	C8	C	140.4595	0.0000	1
260	1	49	U	H1'	H	5.5111	0.0000	1
261	1	49	U	H2'	H	4.6159	0.0000	1
262	1	49	U	H3'	H	4.5504	0.0000	1
263	1	49	U	H5	H	5.0925	0.0000	1
264	1	49	U	H6	H	7.6788	0.0000	1
265	1	50	G	H1'	H	5.8430	0.0000	1
266	1	50	G	H2'	H	4.5624	0.0000	1
267	1	50	G	H3'	H	4.5858	0.0000	1
268	1	50	G	H8	H	7.7381	0.0000	1
269	1	50	G	C8	C	136.9487	0.0000	1
270	1	51	C	H1'	H	5.5354	0.0000	1
271	1	51	C	H2'	H	4.5656	0.0000	1
272	1	51	C	H3'	H	4.6046	0.0000	1
273	1	51	C	H5	H	5.5327	0.0000	1
274	1	51	C	H6	H	7.7575	0.0000	1
275	1	52	C	H1'	H	5.5459	0.0000	1
276	1	52	C	H2'	H	4.6193	0.0000	1
277	1	52	C	H3'	H	4.5992	0.0000	1
278	1	52	C	H5	H	5.2498	0.0000	1
279	1	52	C	H6	H	7.7244	0.0000	1
280	1	53	A	H1'	H	5.9235	0.0000	1
281	1	53	A	H2	H	6.6383	0.0000	1
282	1	53	A	H2'	H	4.6063	0.0000	1
283	1	53	A	H3'	H	4.5781	0.0000	1
284	1	53	A	H8	H	7.9696	0.0000	1
285	1	53	A	C8	C	139.8936	0.0000	1
286	1	54	A	H1'	H	6.0625	0.0000	1
287	1	54	A	H2	H	8.1289	0.0000	1
288	1	54	A	H2'	H	4.5910	0.0000	1
289	1	54	A	H3'	H	4.6418	0.0000	1
290	1	54	A	H8	H	7.9940	0.0000	1
291	1	54	A	C2	C	155.9456	0.0000	1
292	1	54	A	C8	C	140.1449	0.0000	1
293	1	55	C	H1'	H	5.4409	0.0000	1
294	1	55	C	H2'	H	4.5555	0.0000	1
295	1	55	C	H3'	H	4.5720	0.0000	1
296	1	55	C	H5	H	5.3717	0.0000	1
297	1	55	C	H6	H	7.5963	0.0000	1
298	1	56	A	H1'	H	5.9943	0.0000	1
299	1	56	A	H2	H	7.3357	0.0000	1
300	1	56	A	H2'	H	4.5408	0.0000	1
301	1	56	A	H3'	H	4.5882	0.0000	1
302	1	56	A	H8	H	8.0007	0.0000	1
303	1	56	A	C2	C	153.9304	0.0000	1
304	1	56	A	C8	C	140.3163	0.0000	1
305	1	57	U	H1'	H	5.5589	0.0000	1
306	1	57	U	H2'	H	4.6321	0.0000	1
307	1	57	U	H3'	H	4.5936	0.0000	1
308	1	57	U	H5	H	5.0401	0.0000	1
309	1	57	U	H6	H	7.5977	0.0000	1
310	1	58	A	H1'	H	6.1027	0.0000	1
311	1	58	A	H2	H	7.6554	0.0000	1
312	1	58	A	H2'	H	4.5800	0.0000	1
313	1	58	A	H3'	H	4.5538	0.0000	1
314	1	58	A	H8	H	8.1898	0.0000	1
315	1	58	A	C2	C	154.6613	0.0000	1
316	1	58	A	C8	C	140.8530	0.0000	1
317	1	59	U	H1'	H	5.3081	0.0000	1
318	1	59	U	H2'	H	4.5262	0.0000	1
319	1	59	U	H3'	H	4.5155	0.0000	1
320	1	59	U	H5	H	5.3633	0.0000	1
321	1	59	U	H6	H	7.4581	0.0000	1
322	1	60	U	H1'	H	5.7056	0.0000	1
323	1	60	U	H2'	H	4.5763	0.0000	1
324	1	60	U	H3'	H	4.6085	0.0000	1
325	1	60	U	H5	H	5.5741	0.0000	1
326	1	60	U	H6	H	8.0235	0.0000	1
327	1	61	G	H1'	H	5.8281	0.0000	1
328	1	61	G	H2'	H	4.5991	0.0000	1
329	1	61	G	H3'	H	4.4892	0.0000	1
330	1	61	G	H8	H	7.8045	0.0000	1
331	1	61	G	C8	C	137.0593	0.0000	1
332	1	62	C	H1'	H	5.5235	0.0000	1
333	1	62	C	H2'	H	4.6663	0.0000	1
334	1	62	C	H3'	H	4.4945	0.0000	1
335	1	62	C	H5	H	5.2776	0.0000	1
336	1	62	C	H6	H	7.7057	0.0000	1
337	1	63	C	H1'	H	5.4854	0.0000	1
338	1	63	C	H2'	H	4.6298	0.0000	1
339	1	63	C	H3'	H	4.5161	0.0000	1
340	1	63	C	H5	H	5.5151	0.0000	1
341	1	63	C	H6	H	7.6768	0.0000	1
342	1	64	A	H1'	H	6.0701	0.0000	1
343	1	64	A	H2	H	8.4490	0.0000	1
344	1	64	A	H2'	H	4.5985	0.0000	1
345	1	64	A	H3'	H	4.5191	0.0000	1
346	1	64	A	H8	H	8.2603	0.0000	1
347	1	64	A	C2	C	156.4797	0.0000	1
348	1	64	A	C8	C	140.5053	0.0000	1
349	1	65	U	H1'	H	5.5718	0.0000	1
350	1	65	U	H2'	H	4.5397	0.0000	1
351	1	65	U	H3'	H	4.5114	0.0000	1
352	1	65	U	H5	H	5.1218	0.0000	1
353	1	65	U	H6	H	7.4872	0.0000	1
354	1	66	C	H1'	H	5.6486	0.0000	1
355	1	66	C	H2'	H	4.2139	0.0000	1
356	1	66	C	H3'	H	4.5722	0.0000	1
357	1	66	C	H5	H	5.7264	0.0000	1
358	1	66	C	H6	H	8.0725	0.0000	1
359	1	67	U	H1'	H	5.5752	0.0000	1
360	1	67	U	H2'	H	4.5780	0.0000	1
361	1	67	U	H3'	H	4.6502	0.0000	1
362	1	67	U	H5	H	5.4729	0.0000	1
363	1	67	U	H6	H	7.8516	0.0000	1
364	1	68	U	H1'	H	5.6179	0.0000	1
365	1	68	U	H2'	H	4.6608	0.0000	1
366	1	68	U	H3'	H	4.5234	0.0000	1
367	1	68	U	H5	H	5.8935	0.0000	1
368	1	68	U	H6	H	8.0886	0.0000	1
369	1	69	U	H1'	H	5.5973	0.0000	1
370	1	69	U	H2'	H	4.6981	0.0000	1
371	1	69	U	H3'	H	4.5778	0.0000	1
372	1	69	U	H5	H	5.7124	0.0000	1
373	1	69	U	H6	H	8.2267	0.0000	1
374	1	70	C	H1'	H	5.6773	0.0000	1
375	1	70	C	H2'	H	4.5364	0.0000	1
376	1	70	C	H3'	H	4.5862	0.0000	1
377	1	70	C	H5	H	5.7732	0.0000	1
378	1	70	C	H6	H	8.0006	0.0000	1
379	1	71	C	H1'	H	5.8407	0.0000	1
380	1	71	C	H2'	H	4.2734	0.0000	1
381	1	71	C	H3'	H	4.6427	0.0000	1
382	1	71	C	H5	H	5.5769	0.0000	1
383	1	71	C	H6	H	7.7761	0.0000	1

Release date

2022-12-25

Citation

Structure of pre-miR-31 reveals an active role in Dicer processing
Kotar, A., Ma, S., Keane, S.

Experiments performed 12 experiments Detail

Chemical shift validation 3 contents Detail