BMRB: 31079

Structural and functional characterisation of Tst2, a novel TRPV1 inhibitory peptide from the Australian sea anemone Telmatactis stephensoni

Authors

Elnahriry, K.A., Wai, D.C.C., Norton, R.S.

Assembly

TRPV1 inhibitory peptide Tst2

Entity

1. TRPV1 inhibitory peptide Tst2 (polymer, Thiol state: all disulfide bound), 39 monomers, 4028.657 Da Detail

GCKGQDAPCS KSKDCCGEAS MCSKGADGKK TCMIDKLWG

Formula weight

4028.657 Da

Entity Connection

disulfide 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS16:SG
2	disulfide	sing	1:CYS9:SG	1:CYS22:SG
3	disulfide	sing	1:CYS9:SG	1:CYS32:SG
4	disulfide	sing	1:CYS15:SG	1:CYS32:SG

Source organism

Telmatactis stephensoni

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chemcal Shifts

¹H: 200, ¹³C: 110, ¹⁵N: 39 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
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• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	GLY	HA2	H	3.889	0
2	1	1	GLY	C	C	166.969	0
3	1	1	GLY	CA	C	43.231	12
4	1	2	CYS	H	H	8.601	10
5	1	2	CYS	HA	H	4.801	0
6	1	2	CYS	HB2	H	3.168	11
7	1	2	CYS	HB3	H	3.017	9
8	1	2	CYS	C	C	170.966	0
9	1	2	CYS	CA	C	54.728	103
10	1	2	CYS	CB	C	42.421	25
11	1	2	CYS	N	N	117.217	8
12	1	3	LYS	H	H	9.044	10
13	1	3	LYS	HA	H	4.481	0
14	1	3	LYS	HB2	H	1.932	2
15	1	3	LYS	HB3	H	1.827	0
16	1	3	LYS	HG2	H	1.503	2
17	1	3	LYS	HG3	H	1.398	2
18	1	3	LYS	HD2	H	1.609	3
19	1	3	LYS	HE2	H	2.895	140
20	1	3	LYS	C	C	172.505	0
21	1	3	LYS	CA	C	55.038	106
22	1	3	LYS	CB	C	34.254	57
23	1	3	LYS	N	N	121.457	59
24	1	4	GLY	H	H	8.373	7
25	1	4	GLY	HA2	H	4.170	2
26	1	4	GLY	HA3	H	3.657	1
27	1	4	GLY	C	C	169.343	0
28	1	4	GLY	CA	C	43.277	35
29	1	4	GLY	N	N	108.702	141
30	1	5	GLN	H	H	8.162	9
31	1	5	GLN	HA	H	3.680	12
32	1	5	GLN	HB2	H	2.078	19
33	1	5	GLN	HB3	H	1.811	3
34	1	5	GLN	HG2	H	2.442	11
35	1	5	GLN	HG3	H	2.260	11
36	1	5	GLN	HE21	H	7.741	22
37	1	5	GLN	HE22	H	6.960	12
38	1	5	GLN	C	C	174.636	0
39	1	5	GLN	CA	C	57.197	38
40	1	5	GLN	CB	C	28.241	20
41	1	5	GLN	N	N	117.832	52
42	1	5	GLN	NE2	N	115.444	1
43	1	6	ASP	H	H	9.277	7
44	1	6	ASP	HA	H	4.216	16
45	1	6	ASP	HB2	H	3.254	22
46	1	6	ASP	HB3	H	3.030	1
47	1	6	ASP	C	C	170.949	0
48	1	6	ASP	CA	C	57.337	52
49	1	6	ASP	CB	C	39.317	37
50	1	6	ASP	N	N	119.842	61
51	1	7	ALA	H	H	8.012	14
52	1	7	ALA	HA	H	4.623	1
53	1	7	ALA	HB1	H	1.400	2
54	1	7	ALA	HB2	H	1.400	2
55	1	7	ALA	HB3	H	1.400	2
56	1	7	ALA	CA	C	50.679	0
57	1	7	ALA	CB	C	19.597	0
58	1	7	ALA	N	N	123.980	28
59	1	8	PRO	HA	H	4.820	16
60	1	8	PRO	HB2	H	2.403	8
61	1	8	PRO	HB3	H	2.087	8
62	1	8	PRO	HG2	H	1.979	13
63	1	8	PRO	HG3	H	1.824	8
64	1	8	PRO	HD2	H	3.919	7
65	1	8	PRO	HD3	H	3.599	8
66	1	8	PRO	C	C	172.817	1
67	1	8	PRO	CA	C	62.906	22
68	1	8	PRO	CB	C	32.471	2
69	1	8	PRO	CD	C	50.427	8
70	1	9	CYS	H	H	7.695	14
71	1	9	CYS	HA	H	4.861	32
72	1	9	CYS	HB2	H	3.261	3
73	1	9	CYS	HB3	H	3.161	6
74	1	9	CYS	C	C	169.620	0
75	1	9	CYS	CA	C	53.465	47
76	1	9	CYS	CB	C	47.024	49
77	1	9	CYS	N	N	114.896	113
78	1	10	SER	H	H	9.153	15
79	1	10	SER	HA	H	4.578	3
80	1	10	SER	HB2	H	3.864	1
81	1	10	SER	C	C	170.715	0
82	1	10	SER	CA	C	59.125	55
83	1	10	SER	CB	C	65.087	12
84	1	10	SER	N	N	113.461	176
85	1	11	LYS	H	H	8.025	12
86	1	11	LYS	HA	H	4.877	0
87	1	11	LYS	HB2	H	1.980	0
88	1	11	LYS	HB3	H	1.840	1
89	1	11	LYS	HG2	H	1.443	8
90	1	11	LYS	HG3	H	1.166	26
91	1	11	LYS	HD2	H	1.651	0
92	1	11	LYS	HE2	H	2.880	0
93	1	11	LYS	C	C	174.441	0
94	1	11	LYS	CA	C	54.264	54
95	1	11	LYS	CB	C	35.314	2
96	1	11	LYS	N	N	120.144	41
97	1	12	SER	H	H	9.249	12
98	1	12	SER	HA	H	4.748	0
99	1	12	SER	HB2	H	4.049	10
100	1	12	SER	HB3	H	3.955	8
101	1	12	SER	C	C	173.129	0
102	1	12	SER	CA	C	63.303	119
103	1	12	SER	CB	C	62.076	100
104	1	12	SER	N	N	120.998	27
105	1	13	LYS	H	H	8.118	10
106	1	13	LYS	HA	H	4.151	57
107	1	13	LYS	HB2	H	1.983	0
108	1	13	LYS	HB3	H	1.843	0
109	1	13	LYS	HG2	H	1.166	0
110	1	13	LYS	HD2	H	1.735	5
111	1	13	LYS	HD3	H	1.482	9
112	1	13	LYS	HE2	H	2.979	0
113	1	13	LYS	C	C	173.643	0
114	1	13	LYS	CA	C	57.429	34
115	1	13	LYS	CB	C	31.867	5
116	1	13	LYS	N	N	118.496	81
117	1	14	ASP	H	H	7.826	12
118	1	14	ASP	HA	H	4.361	47
119	1	14	ASP	HB2	H	3.037	21
120	1	14	ASP	HB3	H	2.770	36
121	1	14	ASP	C	C	174.231	0
122	1	14	ASP	CA	C	56.353	101
123	1	14	ASP	CB	C	41.949	104
124	1	14	ASP	N	N	117.897	112
125	1	15	CYS	H	H	7.892	13
126	1	15	CYS	HA	H	5.095	4
127	1	15	CYS	HB2	H	2.997	6
128	1	15	CYS	HB3	H	2.860	1
129	1	15	CYS	C	C	171.703	0
130	1	15	CYS	CA	C	53.497	46
131	1	15	CYS	CB	C	39.192	18
132	1	15	CYS	N	N	118.547	57
133	1	16	CYS	H	H	9.458	10
134	1	16	CYS	HA	H	4.485	9
135	1	16	CYS	HB2	H	3.074	1
136	1	16	CYS	HB3	H	2.515	1
137	1	16	CYS	C	C	172.596	0
138	1	16	CYS	CA	C	55.029	76
139	1	16	CYS	CB	C	40.218	94
140	1	16	CYS	N	N	122.570	55
141	1	17	GLY	H	H	8.531	6
142	1	17	GLY	HA2	H	4.271	6
143	1	17	GLY	HA3	H	3.653	2
144	1	17	GLY	C	C	171.764	0
145	1	17	GLY	CA	C	44.850	6
146	1	17	GLY	N	N	110.945	23
147	1	18	GLU	H	H	8.600	20
148	1	18	GLU	HA	H	4.143	9
149	1	18	GLU	HB2	H	2.035	2
150	1	18	GLU	HB3	H	1.968	1
151	1	18	GLU	HG2	H	2.349	1
152	1	18	GLU	C	C	174.204	0
153	1	18	GLU	CA	C	58.243	94
154	1	18	GLU	CB	C	29.688	54
155	1	18	GLU	N	N	122.668	46
156	1	19	ALA	H	H	8.555	21
157	1	19	ALA	HA	H	4.446	59
158	1	19	ALA	HB1	H	1.310	29
159	1	19	ALA	HB2	H	1.310	29
160	1	19	ALA	HB3	H	1.310	29
161	1	19	ALA	C	C	173.832	0
162	1	19	ALA	CA	C	51.391	56
163	1	19	ALA	CB	C	18.351	32
164	1	19	ALA	N	N	122.523	28
165	1	20	SER	H	H	7.276	6
166	1	20	SER	HA	H	4.639	4
167	1	20	SER	HB2	H	3.700	47
168	1	20	SER	HB3	H	3.538	57
169	1	20	SER	C	C	169.547	0
170	1	20	SER	CA	C	58.339	106
171	1	20	SER	CB	C	63.972	51
172	1	20	SER	N	N	115.774	47
173	1	21	MET	H	H	8.461	6
174	1	21	MET	HB2	H	1.917	0
175	1	21	MET	HB3	H	1.855	0
176	1	21	MET	HG2	H	2.410	2
177	1	21	MET	C	C	170.796	0
178	1	21	MET	CA	C	53.771	12
179	1	21	MET	CB	C	36.470	32
180	1	21	MET	N	N	118.029	88
181	1	22	CYS	H	H	8.487	12
182	1	22	CYS	HA	H	4.935	0
183	1	22	CYS	HB2	H	2.654	1
184	1	22	CYS	HB3	H	2.485	1
185	1	22	CYS	C	C	171.131	0
186	1	22	CYS	CA	C	54.984	100
187	1	22	CYS	CB	C	39.489	19
188	1	22	CYS	N	N	123.921	57
189	1	23	SER	H	H	8.631	14
190	1	23	SER	HA	H	4.661	0
191	1	23	SER	HB2	H	3.784	11
192	1	23	SER	HB3	H	3.547	7
193	1	23	SER	C	C	170.620	0
194	1	23	SER	CA	C	57.362	35
195	1	23	SER	CB	C	65.693	56
196	1	23	SER	N	N	124.131	59
197	1	24	LYS	H	H	8.698	11
198	1	24	LYS	HA	H	4.296	0
199	1	24	LYS	HB2	H	1.801	2
200	1	24	LYS	HG2	H	1.340	117
201	1	24	LYS	HG3	H	0.964	1
202	1	24	LYS	HD3	H	1.593	12
203	1	24	LYS	HE2	H	2.979	0
204	1	24	LYS	C	C	173.935	0
205	1	24	LYS	CA	C	56.985	95
206	1	24	LYS	CB	C	33.046	29
207	1	24	LYS	N	N	126.751	37
208	1	25	GLY	H	H	8.554	18
209	1	25	GLY	HA2	H	4.469	19
210	1	25	GLY	HA3	H	3.954	1
211	1	25	GLY	C	C	172.798	0
212	1	25	GLY	CA	C	44.043	48
213	1	25	GLY	N	N	114.910	27
214	1	26	ALA	H	H	8.832	14
215	1	26	ALA	HA	H	4.090	11
216	1	26	ALA	HB1	H	1.418	0
217	1	26	ALA	HB2	H	1.418	0
218	1	26	ALA	HB3	H	1.418	0
219	1	26	ALA	C	C	175.589	0
220	1	26	ALA	CA	C	54.672	67
221	1	26	ALA	CB	C	18.358	58
222	1	26	ALA	N	N	124.405	26
223	1	27	ASP	H	H	8.192	7
224	1	27	ASP	HA	H	4.467	11
225	1	27	ASP	HB2	H	2.982	3
226	1	27	ASP	HB3	H	2.671	3
227	1	27	ASP	C	C	174.321	0
228	1	27	ASP	CA	C	53.177	112
229	1	27	ASP	CB	C	39.960	127
230	1	27	ASP	N	N	114.702	117
231	1	28	GLY	H	H	8.103	7
232	1	28	GLY	HA2	H	4.250	1
233	1	28	GLY	HA3	H	3.495	1
234	1	28	GLY	C	C	171.044	0
235	1	28	GLY	CA	C	45.466	55
236	1	28	GLY	N	N	107.442	31
237	1	29	LYS	H	H	7.824	16
238	1	29	LYS	HA	H	4.385	15
239	1	29	LYS	HB2	H	1.847	0
240	1	29	LYS	HB3	H	1.622	0
241	1	29	LYS	HG2	H	1.276	0
242	1	29	LYS	HG3	H	1.168	0
243	1	29	LYS	HD2	H	1.632	0
244	1	29	LYS	HE2	H	2.893	26
245	1	29	LYS	C	C	173.759	0
246	1	29	LYS	CA	C	55.892	33
247	1	29	LYS	CB	C	32.857	32
248	1	29	LYS	N	N	121.089	35
249	1	30	LYS	H	H	8.428	10
250	1	30	LYS	HA	H	4.958	5
251	1	30	LYS	HB2	H	1.849	0
252	1	30	LYS	HB3	H	1.746	9
253	1	30	LYS	HG2	H	1.282	0
254	1	30	LYS	HG3	H	0.955	11
255	1	30	LYS	HD3	H	1.498	7
256	1	30	LYS	HE2	H	2.900	0
257	1	30	LYS	C	C	174.358	0
258	1	30	LYS	CA	C	56.188	70
259	1	30	LYS	CB	C	33.414	63
260	1	30	LYS	N	N	124.089	58
261	1	31	THR	H	H	8.433	10
262	1	31	THR	HA	H	5.184	13
263	1	31	THR	HG21	H	0.905	34
264	1	31	THR	HG22	H	0.905	34
265	1	31	THR	HG23	H	0.905	34
266	1	31	THR	C	C	172.542	0
267	1	31	THR	CA	C	59.896	68
268	1	31	THR	CB	C	73.736	24
269	1	31	THR	N	N	114.083	129
270	1	32	CYS	H	H	8.625	14
271	1	32	CYS	HA	H	4.818	1
272	1	32	CYS	HB2	H	3.269	6
273	1	32	CYS	HB3	H	2.615	3
274	1	32	CYS	C	C	172.573	0
275	1	32	CYS	CA	C	56.230	83
276	1	32	CYS	CB	C	40.338	26
277	1	32	CYS	N	N	118.256	12
278	1	33	MET	H	H	9.397	13
279	1	33	MET	HA	H	4.915	7
280	1	33	MET	HB2	H	2.052	2
281	1	33	MET	HG2	H	2.622	8
282	1	33	MET	HG3	H	2.495	5
283	1	33	MET	C	C	172.433	0
284	1	33	MET	CA	C	54.066	36
285	1	33	MET	CB	C	33.583	32
286	1	33	MET	N	N	124.970	32
287	1	34	ILE	H	H	8.632	12
288	1	34	ILE	HA	H	4.313	8
289	1	34	ILE	HB	H	1.967	8
290	1	34	ILE	HG12	H	1.362	32
291	1	34	ILE	HG21	H	0.868	4
292	1	34	ILE	HG22	H	0.868	4
293	1	34	ILE	HG23	H	0.868	4
294	1	34	ILE	C	C	173.340	0
295	1	34	ILE	CA	C	59.810	48
296	1	34	ILE	CB	C	37.736	44
297	1	34	ILE	N	N	124.678	49
298	1	35	ASP	H	H	8.431	8
299	1	35	ASP	HA	H	4.497	8
300	1	35	ASP	HB2	H	2.699	10
301	1	35	ASP	HB3	H	2.578	1
302	1	35	ASP	C	C	173.151	0
303	1	35	ASP	CA	C	54.292	126
304	1	35	ASP	CB	C	40.980	75
305	1	35	ASP	N	N	125.235	138
306	1	36	LYS	H	H	8.198	7
307	1	36	LYS	HA	H	4.338	140
308	1	36	LYS	HB2	H	1.720	2
309	1	36	LYS	HB3	H	1.636	1
310	1	36	LYS	HG3	H	1.298	4
311	1	36	LYS	HE2	H	2.656	0
312	1	36	LYS	C	C	173.564	0
313	1	36	LYS	CA	C	56.427	124
314	1	36	LYS	CB	C	32.871	54
315	1	36	LYS	N	N	122.516	96
316	1	37	LEU	H	H	8.177	23
317	1	37	LEU	HA	H	4.178	8
318	1	37	LEU	HB2	H	1.457	9
319	1	37	LEU	HG	H	1.359	22
320	1	37	LEU	HD11	H	0.856	6
321	1	37	LEU	HD12	H	0.856	6
322	1	37	LEU	HD13	H	0.856	6
323	1	37	LEU	HD21	H	0.745	2
324	1	37	LEU	HD22	H	0.745	2
325	1	37	LEU	HD23	H	0.745	2
326	1	37	LEU	C	C	174.336	0
327	1	37	LEU	CA	C	55.580	32
328	1	37	LEU	CB	C	41.914	37
329	1	37	LEU	N	N	121.882	90
330	1	38	TRP	H	H	7.892	16
331	1	38	TRP	HA	H	4.662	2
332	1	38	TRP	HB2	H	3.345	11
333	1	38	TRP	HB3	H	3.185	1
334	1	38	TRP	HD1	H	7.204	7
335	1	38	TRP	HE1	H	10.098	15
336	1	38	TRP	HE3	H	7.615	7
337	1	38	TRP	HZ2	H	7.459	14
338	1	38	TRP	HZ3	H	7.146	13
339	1	38	TRP	HH2	H	7.205	0
340	1	38	TRP	C	C	172.800	0
341	1	38	TRP	CA	C	56.988	48
342	1	38	TRP	CB	C	29.773	33
343	1	38	TRP	N	N	120.657	38
344	1	38	TRP	NE1	N	129.080	0
345	1	39	GLY	H	H	7.687	10
346	1	39	GLY	HA2	H	3.701	17
347	1	39	GLY	HA3	H	3.596	8
348	1	39	GLY	CA	C	46.102	0
349	1	39	GLY	N	N	115.891	111

Release date

2023-10-01

Citation

Structural and functional characterisation of Tst2, a novel TRPV1 inhibitory peptide from the Australian sea anemone Telmatactis stephensoni
Elnahriry, K.A., Wai, D.C.C., Ashwood, L.M., Naseem, M.U., Szanto, T.G., Guo, S., Panyi, G., Prentis, P.J., Norton, R.S.
Biochim. Biophys. Acta. Proteins Proteom. (2023), 1872, 140952-140952, PubMed 37640250 , DOI 10.1016/j.bbapap.2023.140952 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. TRPV1 inhibitory peptide Tst2, : 4 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail