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Site-specific Aspartic Acid Dehydration and Isomerization in Streptococcal Protein GB1: D-isoAsp40 Variant
Authors
Heath, S.L., Guseman, A.J., Gronenborn, A.M., Horne, W.S.
Assembly
Immunoglobulin G-binding protein G
Entity
1. Immunoglobulin G-binding protein G (polymer, Thiol state: not present), 56 monomers, 6222.757 Da Detail

MQYKLILNGK TLKGETTTEA VDAATAEKVF KQYANDNGVX GEWTYDDATK TFTVTE


Formula weight
6222.757 Da
Source organism
Streptococcus
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chemcal Shifts
1H: 386 assignments Detail
IDEntity IDSeq IDComp IDAtom IDAtom typeValVal errAmbig. code
111METHAH4.1770.0021
211METHB2H2.1960.0002
311METHB3H2.0940.0002
411METHG2H2.4290.0001
511METHG3H2.4290.0001
611METHE1H2.1300.0001
711METHE2H2.1300.0001
811METHE3H2.1300.0001
912GLNHH8.3560.0001
1012GLNHAH4.9880.0021
1112GLNHB2H2.0350.0002
1212GLNHB3H1.9910.0002
1312GLNHG2H2.3640.0012
1412GLNHG3H2.1330.0012
1512GLNHE21H6.9190.0002
1612GLNHE22H7.8490.0002
1713TYRHH9.1240.0001
1813TYRHAH5.3880.0001
1913TYRHB2H3.4080.0022
2013TYRHB3H2.7560.0002
2113TYRHD1H7.1510.0001
2213TYRHD2H7.1510.0001
2313TYRHE1H6.9290.0001
2413TYRHE2H6.9290.0001
2514LYSHH9.1060.0011
2614LYSHAH5.2330.0021
2714LYSHB2H2.0480.0012
2814LYSHB3H1.9070.0022
2914LYSHG2H1.4750.0012
3014LYSHG3H1.3520.0012
3114LYSHD2H1.6320.0041
3214LYSHD3H1.6320.0041
3314LYSHE2H2.8560.0022
3414LYSHE3H2.7920.0012
3515LEUHH8.6440.0001
3615LEUHAH4.9430.0021
3715LEUHB2H0.8010.0022
3815LEUHB3H-1.1080.0012
3915LEUHGH0.8810.0021
4015LEUHD11H0.5020.0012
4115LEUHD12H0.5020.0012
4215LEUHD13H0.5020.0012
4315LEUHD21H0.5760.0012
4415LEUHD22H0.5760.0012
4515LEUHD23H0.5760.0012
4616ILEHH9.0740.0011
4716ILEHAH4.2770.0001
4816ILEHBH1.9810.0011
4916ILEHG12H1.4140.0022
5016ILEHG13H1.0900.0012
5116ILEHG21H0.8140.0021
5216ILEHG22H0.8140.0021
5316ILEHG23H0.8140.0021
5416ILEHD11H0.7810.0001
5516ILEHD12H0.7810.0001
5616ILEHD13H0.7810.0001
5717LEUHH8.7020.0011
5817LEUHAH4.4840.0011
5917LEUHB2H1.4730.0022
6017LEUHB3H1.3300.0032
6117LEUHGH1.3370.0001
6217LEUHD11H0.7260.0011
6317LEUHD12H0.7260.0011
6417LEUHD13H0.7260.0011
6517LEUHD21H0.7260.0011
6617LEUHD22H0.7260.0011
6717LEUHD23H0.7260.0011
6818ASNHH9.0170.0001
6918ASNHAH5.2760.0001
7018ASNHB2H2.9710.0022
7118ASNHB3H2.5590.0012
7218ASNHD21H6.7820.0002
7318ASNHD22H7.1770.0002
7419GLYHH8.0200.0001
7519GLYHA2H4.4190.0022
7619GLYHA3H3.9940.0002
77110LYSHH9.4350.0001
78110LYSHAH4.0500.0011
79110LYSHB2H1.8200.0001
80110LYSHB3H1.8200.0001
81110LYSHG2H1.4540.0021
82110LYSHG3H1.4540.0021
83110LYSHD2H1.6720.0011
84110LYSHD3H1.6720.0011
85110LYSHE2H2.9790.0011
86110LYSHE3H2.9790.0011
87111THRHH8.8580.0011
88111THRHAH4.3730.0001
89111THRHBH4.2260.0001
90111THRHG21H1.1410.0021
91111THRHG22H1.1410.0021
92111THRHG23H1.1410.0021
93112LEUHH7.4620.0011
94112LEUHAH4.4690.0021
95112LEUHB2H1.6290.0022
96112LEUHB3H1.4200.0012
97112LEUHGH1.4020.0021
98112LEUHD11H0.7960.0002
99112LEUHD12H0.7960.0002
100112LEUHD13H0.7960.0002
101112LEUHD21H0.8890.0002
102112LEUHD22H0.8890.0002
103112LEUHD23H0.8890.0002
104113LYSHH8.1160.0011
105113LYSHAH5.1570.0001
106113LYSHB2H1.8930.0002
107113LYSHB3H1.7430.0012
108113LYSHG2H1.4490.0002
109113LYSHG3H1.4090.0012
110113LYSHD2H1.6810.0001
111113LYSHD3H1.6810.0001
112113LYSHE2H3.0080.0022
113113LYSHE3H2.9260.0002
114114GLYHH8.4980.0001
115114GLYHA2H4.2330.0001
116114GLYHA3H4.2330.0001
117115GLUHH8.3840.0021
118115GLUHAH5.5560.0011
119115GLUHB2H2.0200.0002
120115GLUHB3H1.9400.0002
121115GLUHG2H2.1520.0011
122115GLUHG3H2.1520.0011
123116THRHH8.7320.0011
124116THRHAH4.7490.0011
125116THRHBH3.9220.0001
126116THRHG21H0.4870.0001
127116THRHG22H0.4870.0001
128116THRHG23H0.4870.0001
129117THRHH8.1110.0001
130117THRHAH5.8190.0001
131117THRHBH4.3100.0001
132117THRHG21H1.1990.0001
133117THRHG22H1.1990.0001
134117THRHG23H1.1990.0001
135118THRHH8.9910.0001
136118THRHAH4.6740.0011
137118THRHBH3.8490.0001
138118THRHG21H0.4850.0021
139118THRHG22H0.4850.0021
140118THRHG23H0.4850.0021
141119GLUHH7.9590.0011
142119GLUHAH5.1850.0011
143119GLUHB2H1.9460.0001
144119GLUHB3H1.9460.0001
145119GLUHG2H2.2930.0022
146119GLUHG3H2.0580.0012
147120ALAHH9.3480.0001
148120ALAHAH4.9450.0011
149120ALAHB1H1.3440.0001
150120ALAHB2H1.3440.0001
151120ALAHB3H1.3440.0001
152121VALHH8.4940.0001
153121VALHAH4.1220.0011
154121VALHBH2.2010.0011
155121VALHG11H1.0090.0001
156121VALHG12H1.0090.0001
157121VALHG13H1.0090.0001
158121VALHG21H1.0090.0001
159121VALHG22H1.0090.0001
160121VALHG23H1.0090.0001
161122ASPHH7.3420.0001
162122ASPHAH4.7620.0021
163122ASPHB2H3.0450.0002
164122ASPHB3H2.9890.0002
165123ALAHH8.2910.0011
166123ALAHAH3.3180.0011
167123ALAHB1H1.1630.0001
168123ALAHB2H1.1630.0001
169123ALAHB3H1.1630.0001
170124ALAHH8.0600.0011
171124ALAHAH3.9780.0011
172124ALAHB1H1.2970.0021
173124ALAHB2H1.2970.0021
174124ALAHB3H1.2970.0021
175125THRHH8.2960.0001
176125THRHAH3.7090.0001
177125THRHBH4.0280.0001
178125THRHG21H1.2310.0011
179125THRHG22H1.2310.0011
180125THRHG23H1.2310.0011
181126ALAHH7.1900.0001
182126ALAHAH3.0970.0011
183126ALAHB1H0.5370.0011
184126ALAHB2H0.5370.0011
185126ALAHB3H0.5370.0011
186127GLUHH8.3410.0001
187127GLUHAH2.6850.0001
188127GLUHB2H1.9710.0002
189127GLUHB3H1.8470.0002
190127GLUHG2H1.6320.0001
191127GLUHG3H1.6320.0001
192128LYSHH6.9540.0001
193128LYSHAH3.7380.0011
194128LYSHB2H1.8390.0011
195128LYSHB3H1.8390.0011
196128LYSHG2H1.5280.0012
197128LYSHG3H1.3340.0012
198128LYSHD2H1.6110.0031
199128LYSHD3H1.6110.0031
200128LYSHE2H2.8960.0021
201128LYSHE3H2.8960.0021
202129VALHH7.3530.0001
203129VALHAH3.6170.0001
204129VALHBH1.7590.0011
205129VALHG11H0.8010.0012
206129VALHG12H0.8010.0012
207129VALHG13H0.8010.0012
208129VALHG21H0.9090.0022
209129VALHG22H0.9090.0022
210129VALHG23H0.9090.0022
211130PHEHH8.5330.0001
212130PHEHAH4.7900.0001
213130PHEHB2H3.3370.0002
214130PHEHB3H2.8850.0002
215130PHEHD1H6.5190.0003
216130PHEHD2H6.5640.0003
217130PHEHE1H7.0840.0011
218130PHEHE2H7.0840.0011
219130PHEHZH7.1900.0011
220131LYSHH9.1280.0001
221131LYSHAH4.1390.0001
222131LYSHB2H1.6560.0042
223131LYSHB3H1.6300.0002
224131LYSHG2H1.0840.0092
225131LYSHG3H0.4910.0012
226131LYSHD2H1.6510.0002
227131LYSHD3H1.5460.0042
228131LYSHE2H1.9460.0011
229131LYSHE3H1.9460.0011
230132GLNHH7.4530.0001
231132GLNHAH4.0150.0011
232132GLNHB2H2.2340.0002
233132GLNHB3H2.1920.0012
234132GLNHG2H2.4310.0002
235132GLNHG3H2.4050.0002
236132GLNHE21H6.8710.0002
237132GLNHE22H7.8920.0002
238133TYRHH8.1540.0001
239133TYRHAH4.2660.0011
240133TYRHB2H3.3090.0012
241133TYRHB3H3.2870.0002
242133TYRHD1H6.9920.0001
243133TYRHD2H6.9920.0001
244133TYRHE1H6.7120.0001
245133TYRHE2H6.7120.0001
246134ALAHH9.1840.0001
247134ALAHAH3.7420.0021
248134ALAHB1H1.7410.0001
249134ALAHB2H1.7410.0001
250134ALAHB3H1.7410.0001
251135ASNHH8.6210.0001
252135ASNHAH4.4090.0011
253135ASNHB2H2.9290.0012
254135ASNHB3H2.8610.0022
255135ASNHD21H6.9880.0002
256135ASNHD22H7.9190.0002
257136ASPHH8.8810.0001
258136ASPHAH4.3850.0011
259136ASPHB2H2.7910.0012
260136ASPHB3H2.5730.0012
261137ASNHH7.4120.0011
262137ASNHAH4.6430.0021
263137ASNHB2H2.8160.0032
264137ASNHB3H2.1600.0002
265137ASNHD21H6.3590.0002
266137ASNHD22H6.7410.0002
267138GLYHH7.7530.0001
268138GLYHA2H4.0900.0002
269138GLYHA3H3.8440.0002
270139VALHH8.3440.0001
271139VALHAH4.1300.0001
272139VALHBH1.6610.0011
273139VALHG11H0.6370.0012
274139VALHG12H0.6370.0012
275139VALHG13H0.6370.0012
276139VALHG21H0.7740.0022
277139VALHG22H0.7740.0022
278139VALHG23H0.7740.0022
279140DASHH8.4730.0011
280140DASHAH4.7480.0021
281140DASHB2H2.7090.0012
282140DASHB3H3.0860.0002
283141GLYHH8.1920.0001
284141GLYHA2H3.8700.0002
285141GLYHA3H3.8210.0002
286142GLUHH8.4930.0011
287142GLUHAH4.8890.0011
288142GLUHB2H2.0380.0012
289142GLUHB3H1.9200.0082
290142GLUHG2H2.2870.0032
291142GLUHG3H2.1990.0002
292143TRPHH9.3070.0001
293143TRPHAH5.2140.0011
294143TRPHB2H3.3580.0012
295143TRPHB3H3.1400.0022
296143TRPHD1H7.2530.0001
297143TRPHE1H10.1980.0011
298143TRPHE3H7.5930.0001
299143TRPHZ2H7.3650.0001
300143TRPHZ3H6.6200.0001
301143TRPHH2H6.7700.0021
302144THRHH9.2140.0001
303144THRHAH4.8280.0001
304144THRHBH4.2250.0001
305144THRHG21H1.2100.0011
306144THRHG22H1.2100.0011
307144THRHG23H1.2100.0011
308145TYRHH8.6030.0001
309145TYRHAH4.9590.0001
310145TYRHB2H2.8800.0002
311145TYRHB3H2.5150.0002
312145TYRHD1H7.1550.0001
313145TYRHD2H7.1550.0001
314145TYRHE1H6.3740.0001
315145TYRHE2H6.3740.0001
316146ASPHH7.6020.0001
317146ASPHAH4.5980.0021
318146ASPHB2H2.6230.0022
319146ASPHB3H2.2720.0012
320147ASPHH8.5970.0001
321147ASPHAH4.1650.0001
322147ASPHB2H2.8410.0002
323147ASPHB3H2.5340.0002
324148ALAHH8.3550.0001
325148ALAHAH4.1260.0001
326148ALAHB1H1.5150.0001
327148ALAHB2H1.5150.0001
328148ALAHB3H1.5150.0001
329149THRHH7.0210.0011
330149THRHAH4.4080.0001
331149THRHBH4.4180.0001
332149THRHG21H1.0870.0011
333149THRHG22H1.0870.0011
334149THRHG23H1.0870.0011
335150LYSHH7.8780.0011
336150LYSHAH4.2140.0031
337150LYSHB2H2.0880.0012
338150LYSHB3H2.0210.0042
339150LYSHG2H1.4050.0022
340150LYSHG3H1.2380.0012
341150LYSHD2H1.7200.0022
342150LYSHD3H1.4200.0022
343150LYSHE2H3.1040.0042
344150LYSHE3H2.9730.0022
345151THRHH7.3940.0001
346151THRHAH5.5170.0001
347151THRHBH3.7800.0011
348151THRHG21H1.0010.0011
349151THRHG22H1.0010.0011
350151THRHG23H1.0010.0011
351152PHEHH10.3580.0001
352152PHEHAH5.6590.0021
353152PHEHB2H3.2760.0032
354152PHEHB3H3.1510.0012
355152PHEHD1H7.8060.0001
356152PHEHD2H7.8060.0001
357152PHEHE1H7.1690.0011
358152PHEHE2H7.1690.0011
359152PHEHZH7.0180.0011
360153THRHH9.1110.0021
361153THRHAH5.1700.0011
362153THRHBH3.8490.0021
363153THRHG21H0.9920.0001
364153THRHG22H0.9920.0001
365153THRHG23H0.9920.0001
366154VALHH8.3950.0001
367154VALHAH4.5730.0031
368154VALHBH-0.0510.0001
369154VALHG11H-0.3120.0012
370154VALHG12H-0.3120.0012
371154VALHG13H-0.3120.0012
372154VALHG21H0.3690.0012
373154VALHG22H0.3690.0012
374154VALHG23H0.3690.0012
375155THRHH8.4040.0011
376155THRHAH4.7370.0021
377155THRHBH3.9450.0001
378155THRHG21H1.2400.0021
379155THRHG22H1.2400.0021
380155THRHG23H1.2400.0021
381156GLUHH7.7220.0011
382156GLUHAH4.2290.0021
383156GLUHB2H2.1790.0012
384156GLUHB3H1.8830.0042
385156GLUHG2H2.2480.0011
386156GLUHG3H2.2480.0011

Release date
2023-10-24
Related entities 1. Immunoglobulin G-binding protein G, : 1 : 3 : 70 : 32 entities Detail
Interaction partners 1. Immunoglobulin G-binding protein G, : 1 interactors Detail
Experiments performed 3 experiments Detail
Chemical shift validation 3 contents Detail
Keywords IMMUNE SYSTEM, immunoglobulin binding domain