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BMRB: 34000

5L3L

D11 bound IGF-II

Authors

Assembly

Insulin-like growth factor II

Entity

1. Insulin-like growth factor II (polymer), 67 monomers, 7475.397 Da Detail

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Formula weight

7475.397 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.4 %, Completeness (bb): 75.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.4 % (628 of 744)	99.7 % (385 of 386)	60.8 % (178 of 293)	100.0 % (65 of 65)
Backbone	75.3 % (298 of 396)	100.0 % (136 of 136)	50.0 % (98 of 196)	100.0 % (64 of 64)
Sidechain	95.1 % (390 of 410)	99.6 % (249 of 250)	88.1 % (140 of 159)	100.0 % (1 of 1)
Aromatic	78.1 % (50 of 64)	100.0 % (32 of 32)	56.3 % (18 of 32)
Methyl	98.4 % (61 of 62)	100.0 % (31 of 31)	96.8 % (30 of 31)

1. Insulin-like growth factor II

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 5L3L, Strand ID: A Detail

Release date

2016-07-31

Citation

Probing Receptor Specificity by Sampling the Conformational Space of the Insulin-like Growth Factor II C-domain
Hexnerova, R., Krizkova, K., Fabry, M., Sieglova, I., Kedrova, K., Collinsova, M., Ullrichova, P., Srb, P., Williams, C., Crump, M.P., Tosner, Z., Jiracek, J., Veverka, V., Zakova, L.
J. Biol. Chem. (2016), 291, 21234-21245, PubMed 27510031 , DOI 10.1074/jbc.M116.741041 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34001 released on 2016-07-31
    Title D11 bound [S39_PQ]-IGF-II
  BMRB: 34002 released on 2016-07-31
    Title D11 bound [N29, S39_PQ]-IGF-II

Related entities 1. Insulin-like growth factor II, : 1 : 15 : 3 : 166 entities Detail

Interaction partners 1. Insulin-like growth factor II, : 8 interactors Detail

More details for 8 interactors identified by 5 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

2 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

3 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

4 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

5 3D HN(CA)CO

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

6 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

7 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

8 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 4.2, Details 20 uM d4 acetic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	acetic acid	d4		20 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

nullKeywords IGF-II, cell cycle