BMRBj - BMREntryMaster

Found 1 Document (1.398 seconds)

BMRB: 34007

5L85

Solution structure of the complex between human ZNHIT3 and NUFIP1 proteins

Authors

Quinternet, M., Chagot, M., Manival, X.

Assembly

Zinc finger HIT domain-containing protein 3, Nuclear fragile X mental retardation-interacting protein 1

Entity

1. Zinc finger HIT domain-containing protein 3 (polymer, Thiol state: all free), 75 monomers, 8483.645 Da Detail

GPHMDRVSLQ NLKNLGESAT LRSLLLNPHL RQLMVNLDQG EDKAKLMRAY MQEPLFVEFA DCCLGIVEPS QNEES

2. Nuclear fragile X mental retardation-interacting protein 1 (polymer, Thiol state: all free), 34 monomers, 4022.588 Da Detail

DIRHERNVIL QCVRYIIKKD FFGLDTNSAK SKDV

Total weight

12506.232 Da

Max. entity weight

8483.645 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.1 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (1247 of 1297)	97.7 % (666 of 682)	93.8 % (466 of 497)	97.5 % (115 of 118)
Backbone	96.7 % (625 of 646)	97.3 % (213 of 219)	96.3 % (310 of 322)	97.1 % (102 of 105)
Sidechain	95.0 % (717 of 755)	96.3 % (446 of 463)	92.5 % (258 of 279)	100.0 % (13 of 13)
Aromatic	70.6 % (48 of 68)	88.2 % (30 of 34)	52.9 % (18 of 34)
Methyl	95.1 % (116 of 122)	93.4 % (57 of 61)	96.7 % (59 of 61)

1. Zinc finger HIT domain-containing protein 3

GPHMDRVSLQ NLKNLGESAT LRSLLLNPHL RQLMVNLDQG EDKAKLMRAY MQEPLFVEFA DCCLGIVEPS QNEES

2. Nuclear fragile X mental retardation-interacting protein 1

DIRHERNVIL QCVRYIIKKD FFGLDTNSAK SKDV

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 1 mM [U-13C; U-15N] ZNHIT3, 1 mM [U-13C; U-15N] NUFIP1, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	NUFIP1	[U-13C; U-15N]	1 mM
2	ZNHIT3	[U-13C; U-15N]	1 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5L85, Strand ID: A, B Detail

Release date

2016-08-25

Citation

Structural Features of the Box C/D snoRNP Pre-assembly Process Are Conserved through Species
Quinternet, M., Chagot, M., Rothe, B., Tiotu, D., Charpentier, B., Manival, X.
Structure (2016), 24, 1693-1706, PubMed 27594683 , DOI 10.1016/j.str.2016.07.016 , Abstract

Related entities 1. Zinc finger HIT domain-containing protein 3, : 3 : 8 entities Detail

Related entities 2. Nuclear fragile X mental retardation-interacting protein 1, : 1 : 2 entities Detail

Interaction partners 1. Zinc finger HIT domain-containing protein 3, : 12 interactors Detail

More details for 12 interactors identified by 6 citations

Experiments performed 14 experiments Detail

1 3D 1H-13C NOESY aliphatic