BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.8 % (1227 of 1336)	93.1 % (645 of 693)	88.9 % (464 of 522)	97.5 % (118 of 121)
Backbone	95.8 % (686 of 716)	95.6 % (238 of 249)	95.2 % (338 of 355)	98.2 % (110 of 112)
Sidechain	88.6 % (646 of 729)	91.7 % (407 of 444)	83.7 % (231 of 276)	88.9 % (8 of 9)
Aromatic	43.5 % (27 of 62)	80.6 % (25 of 31)	3.3 % (1 of 30)	100.0 % (1 of 1)
Methyl	94.4 % (134 of 142)	94.4 % (67 of 71)	94.4 % (67 of 71)

1. BolA-like protein 1

GIDPFTQGSA GSGAIGPVEA AIRTKLEEAL SPEVLELRNE SGGHAVPPGS ETHFRVAVVS SRFEGLSPLQ RHRLVHAALA EELGGPVHAL AIQARTPAQW RENSQLDTSP PCLGGNKKTL GTP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 15N] mitochondrial BOLA1 protein, 50 mM potassium phosphate, 5 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	mitochondrial BOLA1 protein	[U-100% 15N]	1 mM
3	potassium phosphate	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N] mitochondrial BOLA1 protein, 50 mM potassium phosphate, 5 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 1.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 1.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 1.07 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5LCI, Strand ID: A Detail

Release date

2016-08-25

Citation

Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins
Uzarska, M.A., Nasta, V., Weiler, B.D., Spantgar, F., Ciofi-Baffoni, S., Saviello, M.R., Gonnelli, L., M hlenhoff, U., Banci, L., Lill, R.
Elife (2016), 5, e16673-e16673, PubMed 27532772 , DOI 10.7554/eLife.16673 , Abstract

Related entities 1. BolA-like protein 1, : 1 : 2 : 19 entities Detail

Interaction partners 1. BolA-like protein 1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 15N] mitochondrial BOLA1 protein, 50 mM potassium phosphate, 5 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	mitochondrial BOLA1 protein	[U-100% 15N]	1 mM
3	potassium phosphate	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N] mitochondrial BOLA1 protein, 50 mM potassium phosphate, 5 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

4 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

6 3D HBHA(CBCACO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

7 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

8 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

9 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

10 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

11 3D 1H-13C NOESY

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	DTT	natural abundance	5 mM
7	mitochondrial BOLA1 protein	[U-100% 13C; U-100% 15N]	1 mM
8	potassium phosphate	natural abundance	50 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

13 2D 1H-1H TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	mitochondrial BOLA1 protein	[U-100% 15N]	1 mM
3	potassium phosphate	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

14 2D 1H-1H NOESY

Instrument

Bruker Avance - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	mitochondrial BOLA1 protein	[U-100% 15N]	1 mM
3	potassium phosphate	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

15 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	mitochondrial BOLA1 protein	[U-100% 15N]	1 mM
3	potassium phosphate	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_34013_5lci.nef
Input source #2: Coordindates	5lci.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

----20--------30--------40--------50--------60--------70--------80--------90-------100-------110----
GIDPFTQGSAGSGAIGPVEAAIRTKLEEALSPEVLELRNESGGHAVPPGSETHFRVAVVSSRFEGLSPLQRHRLVHAALAEELGGPVHALAIQARTPAQW
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GIDPFTQGSAGSGAIGPVEAAIRTKLEEALSPEVLELRNESGGHAVPPGSETHFRVAVVSSRFEGLSPLQRHRLVHAALAEELGGPVHALAIQARTPAQW
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

---120-------130-------
RENSQLDTSPPCLGGNKKTLGTP
|||||||||||||||||||||||
RENSQLDTSPPCLGGNKKTLGTP
-------110-------120---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	123	0	0	100.0

Content subtype: combined_34013_5lci.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1231		95.9 (chain: A, length: 123)
1	Distance restraints	DYANA/DIANA_distance_constraints_3	OK	1201 (1)	noe	93.5 (chain: A, length: 123)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_2	OK	141 (141)	.	68.3 (chain: A, length: 123)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 95.9%
- Total number of assignments
  - ¹H chemical shifts: 640
  - ¹³C chemical shifts: 465
  - ¹⁵N chemical shifts: 126
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
58	HIS	ND1	205.549
58	HIS	NE2	177.86
67	HIS	ND1	217.336
67	HIS	NE2	175.652
86	HIS	ND1	216.185
86	HIS	NE2	193.886
90	HIS	ND1	223.905
90	HIS	NE2	187.512
102	HIS	ND1	205.591
102	HIS	NE2	178.301

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	693	640	92.4
¹³C chemical shifts	522	465	89.1
¹⁵N chemical shifts	129	116	89.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	249	240	96.4
¹³C chemical shifts	246	234	95.1
¹⁵N chemical shifts	112	109	97.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	444	400	90.1
¹³C chemical shifts	276	231	83.7
¹⁵N chemical shifts	17	7	41.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	69	97.2
¹³C chemical shifts	71	69	97.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	31	27	87.1
¹³C chemical shifts	30	0	0.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 93.5%
- Total number of restraints: 1198
- Weight of restraints: 1.0 (1198)
- Potential type of restraints: upper-bound-parabolic (1198)
- Range of distance target values: 2.4, 6.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 123, Sequence coverage: 68.3%
- Total number of restraints: 141
- Total number of restraints per polymer type: 141
- Weight of restraints: 1.0 (141)
- Potential type of restraints: square-well-parabolic (141)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Fe/S protein biogenesis, UNKNOWN FUNCTION, class II KH-like fold, mitochondrial protein

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Found 1 Document (0.79 seconds)

BMRB: 34013

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. BolA-like protein 1, : 1 : 2 : 19 entities Detail

Interaction partners 1. BolA-like protein 1, : 5 interactors Detail

Experiments performed 15 experiments Detail

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NMR combined restraints 4 contents Detail