BMRB: 34022

Structure of PfIMP2 (Immune Mapped Protein 2 from Plasmodium falciparum) - an antigenic protein

Authors

Benjamin, S.V., Matthews, S.J.

Assembly

Uncharacterized protein

Entity

1. Uncharacterized protein (polymer), 156 monomers, 17582.26 Da Detail

EEKGAYLVFD NASNGTLFIV WKKEKVENAL MFIKPTKEVP EFKFVNRNGK NELIRNLQSD KKLFYSGICQ FVKEAKDIKG KLTLLQHFDS SFPIKVDLYF LKGSKVMPLN TGEPFVVQDI DAMSVLPKGS SSLKVKTMAK DMFVSRGNTE GASISF

Formula weight

17582.26 Da

Source organism

Plasmodium falciparum 3D7

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (1768 of 1908)	92.3 % (922 of 999)	93.7 % (698 of 745)	90.2 % (148 of 164)
Backbone	98.6 % (911 of 924)	97.8 % (309 of 316)	99.6 % (456 of 458)	97.3 % (146 of 150)
Sidechain	88.8 % (1003 of 1130)	89.8 % (613 of 683)	89.6 % (388 of 433)	14.3 % (2 of 14)
Aromatic	51.2 % (87 of 170)	52.9 % (45 of 85)	48.8 % (41 of 84)	100.0 % (1 of 1)
Methyl	100.0 % (166 of 166)	100.0 % (83 of 83)	100.0 % (83 of 83)

1. entity 1

EEKGAYLVFD NASNGTLFIV WKKEKVENAL MFIKPTKEVP EFKFVNRNGK NELIRNLQSD KKLFYSGICQ FVKEAKDIKG KLTLLQHFDS SFPIKVDLYF LKGSKVMPLN TGEPFVVQDI DAMSVLPKGS SSLKVKTMAK DMFVSRGNTE GASISF

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details 1.5 mM [U-13C; U-15N] PfIMP2, 50 mM HEPES, 150 mM sodium chloride, 1 mM DTT, 0.02 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DTT	natural abundance		1 mM
2	HEPES	natural abundance		50 mM
3	PfIMP2	[U-13C; U-15N]		1.5 mM
4	sodium azide	natural abundance		0.02 %
5	sodium chloride	natural abundance		150 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 5LG9, Strand ID: A Detail

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Release date

2016-11-27

Citation 1

NMR assignment of the immune mapped protein 1 (IMP1) homologue from Plasmodium falciparum
Benjamin, S., Williams, F., Kerry, L., Matthews, S.
Biomol. NMR Assign. (2015), 9, 393-395, PubMed 25947350 , DOI 10.1007/s12104-015-9616-0 , Abstract

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Citation 2

Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold
Jia, Y., Benjamin, S., Liu, Q., Xu, Y., Dogga, S.K., Liu, J., Matthews, S., Soldati-Favre, D., Benjamin, S., Williams, F., Kerry, L., Matthews, S.
Biochim. Biophys. Acta (2017), 1865, 208-219, PubMed 27888074 , DOI 10.1016/j.bbapap.2016.11.010 , Abstract

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Related entities 1. Uncharacterized protein, : 1 : 2 entities Detail

Experiments performed 11 experiments Detail