BMRBj - BMREntryMaster

Found 1 Document (0.673 seconds)

BMRB: 34024

5LGM

Gp5.7 mutant L42A

Authors

Liu, B., Matthews, S.

Assembly

Fusion protein 5.5/5.7

Entity

1. Fusion protein 5.5/5.7 (polymer), 69 monomers, 7369.344 Da Detail

MSDYLKVLQA IKSCPKTFQS NYVRNNASLV AEAASRGHIS CATTSGRNGG AWEITASGTR FLKRMGGCV

Formula weight

7369.344 Da

Source organism

Escherichia phage T7

Exptl. method

Refine. method

distance geometrysimulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 90.5 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.5 % (684 of 756)	89.0 % (347 of 390)	92.8 % (270 of 291)	89.3 % (67 of 75)
Backbone	95.4 % (393 of 412)	95.1 % (137 of 144)	95.0 % (190 of 200)	97.1 % (66 of 68)
Sidechain	86.5 % (351 of 406)	85.4 % (210 of 246)	91.5 % (140 of 153)	14.3 % (1 of 7)
Aromatic	86.5 % (45 of 52)	88.5 % (23 of 26)	84.0 % (21 of 25)	100.0 % (1 of 1)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. entity 1

MSDYLKVLQA IKSCPKTFQS NYVRNNASLV AEAASRGHIS CATTSGRNGG AWEITASGTR FLKRMGGCV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.46 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5LGM, Strand ID: A Detail

Release date

2017-08-07

Citation

A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement
Liu, B., Shadrin, A., Sheppard, C., Mekler, V., Xu, Y., Severinov, K., Matthews, S., Wigneshweraraj, S.
Nucleic Acids Res. (2014), 42, 4294-4305, PubMed 24482445 , DOI 10.1093/nar/gku080 , Abstract

Related entities 1. Fusion protein 5.5/5.7, : 2 entities Detail

Experiments performed 10 experiments Detail

1 HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

2 3D HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

3 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

4 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

5 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

6 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

7 3D H(CCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

8 3D 1H-13C NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

9 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

10 3D HBHA(CO)NH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 300 mM NA sodium chloride, 50 mM NA sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	sodium chloride	natural abundance		300 mM
3	sodium phosphate	natural abundance		50 mM

nullKeywords Transcription effector, anti-sigma effector, transcription