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BMRB: 34029

5LMY

Solution structure of the m-pmv myristoylated matrix protein

Authors

Prchal, J., Hrabal, R.

Assembly

Matrix protein p10

Entity

1. Matrix protein p10 (polymer), 125 monomers, 14899.79 Da Detail

XGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH

Formula weight

14899.79 Da

Source organism

Mason-Pfizer monkey virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 67.0 %, Completeness (bb): 90.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.0 % (1028 of 1535)	59.7 % (479 of 803)	72.0 % (430 of 597)	88.1 % (119 of 135)
Backbone	90.2 % (662 of 734)	90.3 % (224 of 248)	87.7 % (322 of 367)	97.5 % (116 of 119)
Sidechain	52.0 % (478 of 920)	45.9 % (255 of 555)	63.0 % (220 of 349)	18.8 % (3 of 16)
Aromatic	28.7 % (50 of 174)	51.7 % (45 of 87)	4.8 % (4 of 84)	33.3 % (1 of 3)
Methyl	73.8 % (90 of 122)	73.8 % (45 of 61)	73.8 % (45 of 61)

1. entity 1

XGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 1.2 mM [U-99% 13C; U-99% 15N] Matrix protein p10, 100 mM potassium phosphate, 300 mM sodium chloride, 5 mM DTT, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.2 mM [U-99% 15N] Matrix protein p10, 50 mM potassium phosphate, 150 mM sodium chloride, 5 mM DTT, 10 mg/mL Pf1 phage, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
5	DTT	natural abundance	5 mM
6	Matrix protein p10	[U-99% 15N]	0.2 mM
7	Pf1 phage	natural abundance	10 mg/mL
8	potassium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 5LMY, Strand ID: A Detail

Release date

2016-09-29

Citation

The structure of myristoylated Mason-Pfizer monkey virus matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in its membrane binding
Prchal, J., Srb, P., Hunter, E., Ruml, T., Hrabal, R.
J. Mol. Biol. (2012), 423, 427-438, PubMed 22863803 , DOI 10.1016/j.jmb.2012.07.021 , Abstract

Related entities 1. Matrix protein p10, : 1 : 2 : 20 entities Detail

Interaction partners 1. Matrix protein p10, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 1.2 mM [U-99% 13C; U-99% 15N] Matrix protein p10, 100 mM potassium phosphate, 300 mM sodium chloride, 5 mM DTT, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

2 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

3 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

4 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

5 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

6 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

7 3D HN(CO)CA

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

8 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

9 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

10 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	Matrix protein p10	[U-99% 13C; U-99% 15N]	1.2 mM
3	potassium phosphate	natural abundance	100 mM
4	sodium chloride	natural abundance	300 mM

11 2D 1H-15N HSQC IPAP

Instrument

Bruker AvanceIII - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.2 mM [U-99% 15N] Matrix protein p10, 50 mM potassium phosphate, 150 mM sodium chloride, 5 mM DTT, 10 mg/mL Pf1 phage, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
5	DTT	natural abundance	5 mM
6	Matrix protein p10	[U-99% 15N]	0.2 mM
7	Pf1 phage	natural abundance	10 mg/mL
8	potassium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	150 mM

nullKeywords M-PMV, Matrix, Myristoylated, Retrovirus, Viral protein

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Found 1 Document (0.936 seconds)

BMRB: 34029

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Matrix protein p10, : 1 : 2 : 20 entities Detail

Interaction partners 1. Matrix protein p10, : 1 interactors Detail

Experiments performed 11 experiments Detail

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