Engineering protein stability with atomic precision in a monomeric miniprotein
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 60.2 % (213 of 354) | 83.2 % (158 of 190) | 20.0 % (27 of 135) | 96.6 % (28 of 29) |
Backbone | 56.8 % (100 of 176) | 94.8 % (55 of 58) | 21.7 % (20 of 92) | 96.2 % (25 of 26) |
Sidechain | 57.7 % (120 of 208) | 78.0 % (103 of 132) | 19.2 % (14 of 73) | 100.0 % (3 of 3) |
Methyl | 67.9 % (19 of 28) | 85.7 % (12 of 14) | 50.0 % (7 of 14) |
1. entity 1
XPPTKPTKPG DNATPEKLAK XQADLAKXQK DLADXSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7.4, Details 1 mM PPa-CH3, 8.2 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 13.7 mM NaOH, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | KH2PO4 | natural abundance | 1.8 mM | |
2 | Na2HPO4 | natural abundance | 8.2 mM | |
3 | NaCl | natural abundance | 137 mM | |
4 | NaOH | natural abundance | 13.7 mM | |
5 | PPa-CH3 | natural abundance | 1 mM |