BMRB: 34038

SINEB2 element of the long non-coding RNA activator of translation AS Uchl1

Authors

Podbevsek, P., Plavec, J.

Assembly

RNA (29-MER)

Entity

1. RNA (29-MER) (polymer, Thiol state: not present), 29 monomers, 9372.493 Da Detail

CCUCGUGGUG GUUGUGAACC ACCAUGUGG

Formula weight

9372.493 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 33.5 %, Completeness (bb): 16.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	33.5 % (156 of 465)	36.6 % (93 of 254)	32.6 % (63 of 193)	0.0 % (0 of 18)
Suger, PO4	16.3 % (52 of 319)	18.4 % (32 of 174)	13.8 % (20 of 145)
Nucleobase	71.2 % (104 of 146)	76.2 % (61 of 80)	89.6 % (43 of 48)	0.0 % (0 of 18)
Aromatic	72.7 % (96 of 132)	80.3 % (53 of 66)	89.6 % (43 of 48)	0.0 % (0 of 18)

1. entity 1

CCUCGUGGUG GUUGUGAACC ACCAUGUGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.5 mM RNA (29-MER), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (29-MER)	natural abundance		0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.5 mM RNA (29-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	RNA (29-MER)	natural abundance		0.5 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.5 mM [U-99% 13C; U-99% 15N] RNA (29-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	RNA (29-MER)	[U-99% 13C; U-99% 15N]		0.5 mM

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Release date

2016-09-05

Citation

Structural determinants of the SINE B2 element embedded in the long non-coding RNA activator of translation AS Uchl1
Podbevsek, P., Fasolo, F., Bon, C., Cimatti, L., Reisser, S., Carninci, P., Bussi, G., Zucchelli, S., Plavec, J., Gustincich, S.
Sci. Rep. (2018), 8, 3189-3189, PubMed 29453387 , DOI 10.1038/s41598-017-14908-6 , Abstract

Experiments performed 6 experiments Detail