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BMRB: 34041

5LVF

Solution structure of Rtt103 CTD-interacting domain bound to a Thr4 phosphorylated CTD peptide

Authors

Jasnovidova, O., Kubicek, K., Krejcikova, M., Stefl, R.

Assembly

Regulator of Ty1 transposition protein 103, PRO-SER-TYR-SER-PRO-PTH-SER-PRO-SER-TYR-SER-PRO-THR-SER-PRO-SER

Entity

1. Regulator of Ty1 transposition protein 103, PRO-SER-TYR-SER-PRO-PTH-SER-PRO-SER-TYR-SER-PRO-THR-SER-PRO-SER, entity 1 (polymer, Thiol state: not present), 142 monomers, 16539.85 Da Detail

MAFSSEQFTT KLNTLEDSQE SISSASKWLL LQYRDAPKVA EMWKEYMLRP SVNTRRKLLG LYLMNHVVQQ AKGQKIIQFQ DSFGKVAAEV LGRINQEFPR DLKKKLSRVV NILKERNIFS KQVVNDIERS LAAALEHHHH HH

2. Regulator of Ty1 transposition protein 103, PRO-SER-TYR-SER-PRO-PTH-SER-PRO-SER-TYR-SER-PRO-THR-SER-PRO-SER, entity 2 (polymer, Thiol state: not present), 16 monomers, 1721.668 Da Detail

PSYSPXSPSY SPTSPS

Total weight

18261.518 Da

Max. entity weight

16539.85 Da

Source organism

Saccharomyces cerevisiae S288C , Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.1 %, Completeness: 78.8 %, Completeness (bb): 74.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.8 % (1502 of 1906)	83.3 % (835 of 1002)	70.7 % (520 of 736)	87.5 % (147 of 168)
Backbone	74.5 % (690 of 926)	84.5 % (262 of 310)	63.8 % (298 of 467)	87.2 % (130 of 149)
Sidechain	83.1 % (941 of 1133)	82.8 % (573 of 692)	83.2 % (351 of 422)	89.5 % (17 of 19)
Aromatic	63.8 % (97 of 152)	71.1 % (54 of 76)	55.4 % (41 of 74)	100.0 % (2 of 2)
Methyl	98.8 % (160 of 162)	98.8 % (80 of 81)	98.8 % (80 of 81)

1. entity 1

MAFSSEQFTT KLNTLEDSQE SISSASKWLL LQYRDAPKVA EMWKEYMLRP SVNTRRKLLG LYLMNHVVQQ AKGQKIIQFQ DSFGKVAAEV LGRINQEFPR DLKKKLSRVV NILKERNIFS KQVVNDIERS LAAALEHHHH HH

2. entity 2

PSYSPXSPSY SPTSPS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 293 K, pH 6.8, Details 1 mM [U-13C; U-15N] Rtt103 CID, 1.2 mM pThr4-CTD, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Rtt103 CID	[U-13C; U-15N]	1 (±0.2) mM
2	pThr4-CTD	natural abundance	1.2 (±0.2) mM
3	KH2PO4	natural abundance	35 mM
4	KCl	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	na	direct	1.0
¹H	water	protons	4.7 ppm	na	direct	1.0
¹⁵N	urea	nitrogen	75.0 ppm	na	direct	1.0

Referencing offset: 2.97 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	na	direct	1.0
¹H	water	protons	4.7 ppm	na	direct	1.0
¹⁵N	urea	nitrogen	75.0 ppm	na	direct	1.0

Referencing offset: 2.97 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	0.0 ppm	na	direct	1.0
¹H	water	protons	4.7 ppm	na	direct	1.0
¹⁵N	urea	nitrogen	75.0 ppm	na	direct	1.0

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5LVF, Strand ID: A, B Detail

Release date

2017-05-03

Citation

Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p
Jasnovidova, O., Krejcikova, M., Kubicek, K., Stefl, R.
EMBO Rep. (2017), 18, 906-913, PubMed 28468956 , DOI 10.15252/embr.201643723 , Abstract

Related entities 1. Regulator of Ty1 transposition protein 103, PRO-SER-TYR-SER-PRO-PTH-SER-PRO-SER-TYR-SER-PRO-THR-SER-PRO-SER, entity 1, : 1 : 43 entities Detail

Related entities 2. Regulator of Ty1 transposition protein 103, PRO-SER-TYR-SER-PRO-PTH-SER-PRO-SER-TYR-SER-PRO-THR-SER-PRO-SER, entity 2, : 4 : 115 entities Detail

Experiments performed 6 experiments Detail

1 3D 1H-15N NOESY