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BMRB: 34042

5LVY

Structural studies of the Aggregative Adherence Fimbriae of Enteroaggregative Escherichia coli

Authors

Liu, B., Matthews, S.

Assembly

Adhesin protein

Entity

1. Adhesin protein (polymer), 154 monomers, 16476.34 Da Detail

NSCSLSISSP DPVTYTIPTD KGDKYINFKL DVPDPRCKAL GGTVYFWGAD TRDGKLVMKK GQDKYTLMTT YGGAVQQQLG GGYGYYHVSQ KTPPQTISGV VSKNVGYKPG QYTVELTGFF SLNDNKQANP TPSSLTSKAA GKNIVSSTGT ITIS

Formula weight

16476.34 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 87.5 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.5 % (1518 of 1734)	85.4 % (766 of 897)	91.4 % (619 of 677)	83.1 % (133 of 160)
Backbone	92.0 % (832 of 904)	91.1 % (288 of 316)	92.8 % (412 of 444)	91.7 % (132 of 144)
Sidechain	84.3 % (814 of 966)	82.3 % (478 of 581)	90.8 % (335 of 369)	6.3 % (1 of 16)
Aromatic	82.4 % (112 of 136)	82.4 % (56 of 68)	82.1 % (55 of 67)	100.0 % (1 of 1)
Methyl	98.1 % (151 of 154)	96.1 % (74 of 77)	100.0 % (77 of 77)

1. entity 1

NSCSLSISSP DPVTYTIPTD KGDKYINFKL DVPDPRCKAL GGTVYFWGAD TRDGKLVMKK GQDKYTLMTT YGGAVQQQLG GGYGYYHVSQ KTPPQTISGV VSKNVGYKPG QYTVELTGFF SLNDNKQANP TPSSLTSKAA GKNIVSSTGT ITIS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

Protein Blocks Logo

Calculated from 10 models in PDB: 5LVY, Strand ID: A Detail

Release date

2017-07-18

Citation

Structural and functional studies of Escherichia coli aggregative adherence fimbriae (AAF/V) reveal a deficiency in extracellular matrix binding
Jnsson, R., Liu, B., Struve, C., Yang, Y., Jrgensen, R., Xu, Y., Jenssen, H., Krogfelt, K.A., Matthews, S.
Biochim. Biophys. Acta (2017), 1865, 304-311, PubMed 27939608 , DOI 10.1016/j.bbapap.2016.11.017 , Abstract

Related entities 1. Adhesin protein, : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

2 3D HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

3 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

4 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

5 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

6 3D HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

7 3D 1H-13C NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

8 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

10 3D H(CCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

11 3D H(CCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.0, Details 50 mM U sodium acetate, 0.05 uM U sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	sodium acetate	natural abundance		50 (±0.2) mM
3	sodium chloride	natural abundance		50 (±0.2) nM

nullKeywords Aggregative adherence fimbriae, CELL ADHESION, E. coli, EAEC, donor strand complementation, fibronectin