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BMRB: 34043

5LW8

Salt-inducible protein splicing by inteins from extremely halophilic archaea and application to scarless segmental labeling of TonB protein

Authors

Iwai, H., Ciragan, A., Tascon, I., Aranko, S.A.

Assembly

Protein TonB

Entity

1. Protein TonB (polymer, Thiol state: not present), 92 monomers, 10137.55 Da Detail

SNEFLMKIQT AISSKNRYPK MAQIRGIEGE VLVSFTINAD GSVTDIKVVK SNTTDILNHA ALEAIKSAAH LFPKPEETVH LKIPIAYSLK ED

Formula weight

10137.55 Da

Source organism

Helicobacter pylori 26695

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 94.4 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.4 % (1014 of 1074)	95.3 % (530 of 556)	92.4 % (391 of 423)	97.9 % (93 of 95)
Backbone	96.9 % (527 of 544)	96.2 % (176 of 183)	97.1 % (265 of 273)	97.7 % (86 of 88)
Sidechain	92.9 % (575 of 619)	94.9 % (354 of 373)	89.5 % (214 of 239)	100.0 % (7 of 7)
Aromatic	56.9 % (33 of 58)	75.9 % (22 of 29)	37.9 % (11 of 29)
Methyl	98.4 % (120 of 122)	96.7 % (59 of 61)	100.0 % (61 of 61)

1. Protein TonB

SNEFLMKIQT AISSKNRYPK MAQIRGIEGE VLVSFTINAD GSVTDIKVVK SNTTDILNHA ALEAIKSAAH LFPKPEETVH LKIPIAYSLK ED

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 mM [U-100% 13C; U-100% 15N] TonB CTD, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	TonB CTD	[U-100% 13C; U-100% 15N]	0.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5LW8, Strand ID: A Detail

Release date

2016-10-13

Citation

Salt-inducible Protein Splicing in cis and trans by Inteins from Extremely Halophilic Archaea as a Novel Protein-Engineering Tool
Ciragan, A., Aranko, A.S., Tascon, I., Iwai, H.
J. Mol. Biol. (2016), 428, 4573-4588, PubMed 27720988 , DOI 10.1016/j.jmb.2016.10.006 , Abstract

Related entities 1. Protein TonB, : 1 : 2 : 1 : 5 entities Detail

Interaction partners 1. Protein TonB, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 3D HNCA