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BMRB: 34047

5LXL

NMR structure of the N-terminal domain of the Bacteriophage T5 decoration protein pb10

Authors

Vernhes, E., Gilquin, B., Cuniasse, P., Boulanger, P., Zinn-justin, S.

Assembly

Decoration protein

Entity

1. Decoration protein (polymer, Thiol state: not present), 86 monomers, 9738.110 Da Detail

MGIDYSGLRT IFGEKLPESH IFFATVAAHK YVPSYAFLRR ELGLSSAHTN RKVWKKFVEA YGKAIPPAPP APPLTLSKLE HHHHHH

Formula weight

9738.11 Da

Source organism

Escherichia virus T5

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.5 %, Completeness: 83.0 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.0 % (857 of 1033)	83.4 % (447 of 536)	81.8 % (341 of 417)	86.3 % (69 of 80)
Backbone	84.6 % (423 of 500)	85.2 % (144 of 169)	83.8 % (212 of 253)	85.9 % (67 of 78)
Sidechain	82.1 % (504 of 614)	82.6 % (303 of 367)	81.2 % (199 of 245)	100.0 % (2 of 2)
Aromatic	73.8 % (96 of 130)	75.4 % (49 of 65)	71.9 % (46 of 64)	100.0 % (1 of 1)
Methyl	90.0 % (81 of 90)	86.7 % (39 of 45)	93.3 % (42 of 45)

1. Decoration protein

MGIDYSGLRT IFGEKLPESH IFFATVAAHK YVPSYAFLRR ELGLSSAHTN RKVWKKFVEA YGKAIPPAPP APPLTLSKLE HHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-99% 13C; U-99% 15N] n77, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	n77	[U-99% 13C; U-99% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	internal	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	nitrogen	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5LXL, Strand ID: A Detail

Release date

2016-11-10

Citation

High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid
Vernhes, E., Renouard, M., Gilquin, B., Cuniasse, P., Durand, D., England, P., Hoos, S., Huet, A., Conway, J.F., Glukhov, A., Ksenzenko, V., Jacquet, E., Nhiri, N., Zinn-Justin, S., Boulanger, P.
Sci. Rep. (2017), 7, 41662-41662, PubMed 28165000 , DOI 10.1038/srep41662 , Abstract

Related entities 1. Decoration protein, : 1 : 1 : 20 entities Detail

Experiments performed 10 experiments Detail

1 2D HSQC