BMRB: 34050

Structure of a Spumaretrovirus Gag central domain reveals an ancient retroviral capsid

Authors

Taylor, I.A., Nicastro, G., Ball, N.

Assembly

Gag protein

Entity

1. Gag protein (polymer), 180 monomers, 19587.29 Da Detail

PIGTVIPIQH IRSVTGEPPR NPREIPIWLG RNAPAIDGVF PVTTPDLRCR IINAILGGNI GLSLTPGDCL TWDSAVATLF IRTHGTFPMH QLGNVIKGIV DQEGVATAYT LGMMLSGQNY QLVSGIIRGY LPGQAVVTAL QQRLDQEIDD QTRAETFIQH LNAVYEILGL NARGQSIRLE

Formula weight

19587.29 Da

Source organism

Human spumaretrovirus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 85.9 %, Completeness (bb): 92.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	85.9 % (1074 of 1250)	84.1 % (891 of 1060)	96.3 % (183 of 190)
Backbone	92.3 % (494 of 535)	89.9 % (330 of 367)	97.6 % (164 of 168)
Sidechain	81.1 % (580 of 715)	81.0 % (561 of 693)	86.4 % (19 of 22)
Aromatic	39.7 % (23 of 58)	41.1 % (23 of 56)	0.0 % (0 of 2)
Methyl	94.4 % (119 of 126)	94.4 % (119 of 126)

1. entity 1

PIGTVIPIQH IRSVTGEPPR NPREIPIWLG RNAPAIDGVF PVTTPDLRCR IINAILGGNI GLSLTPGDCL TWDSAVATLF IRTHGTFPMH QLGNVIKGIV DQEGVATAYT LGMMLSGQNY QLVSGIIRGY LPGQAVVTAL QQRLDQEIDD QTRAETFIQH LNAVYEILGL NARGQSIRLE

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 Pa, Temperature 298 K, pH 7.0, Details 0.3 mM [U-13C; U-15N] protein, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C; U-15N]		0.3 mM
2	NaCl	natural abundance		20 mM
3	Tris	natural abundance		20 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 5M1H, Strand ID: A Detail

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Release date

2016-10-23

Citation

A unique spumavirus Gag N-terminal domain with functional properties of orthoretroviral matrix and capsid
Goldstone, D.C., Flower, T.G., Ball, N.J., Sanz-Ramos, M., Yap, M.W., Ogrodowicz, R.W., Stanke, N., Reh, J., Lindemann, D., Stoye, J.P., Taylor, I.A.
PLoS Pathog. (2013), 9, e1003376-e1003376, PubMed 23675305 , DOI 10.1371/journal.ppat.1003376 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Gag protein, : 5 entities Detail

Experiments performed 3 experiments Detail