BMRBj - BMREntryMaster

Found 1 Document (0.611 seconds)

BMRB: 34053

5M1W

Structure of a stable G-hairpin

Authors

Gajarsky, M., Zivkovic, M.L., Stadlbauer, P., Pagano, B., Fiala, R., Amato, J., Tomaska, L., Sponer, J., Plavec, J., Trantirek, L.

Assembly

DNA (5'-D(*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*G)-3')

Entity

1. DNA (5'-D(*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*G)-3') (polymer, Thiol state: not present), 11 monomers, 3539.226 Da Detail

GTGTGGGTGT G

Formula weight

3539.226 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.3 %, Completeness (bb): 92.2 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	90.3 % (93 of 103)	90.3 % (93 of 103)
Suger, PO4	92.2 % (71 of 77)	92.2 % (71 of 77)
Nucleobase	84.6 % (22 of 26)	84.6 % (22 of 26)
Aromatic	81.8 % (18 of 22)	81.8 % (18 of 22)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. entity 1

GTGTGGGTGT G

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	SC11	natural abundance	0.2 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	10 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.2 mM partial (12%) 15N, 13C residue-specific labelling SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	SC11	partial (12%) 15N, 13C residue-specific labelling	0.2 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	10 mM

Release date

2016-11-10

Citation

Structure of a Stable G-Hairpin
Gajarsky, M., Zivkovic, M.L., Stadlbauer, P., Pagano, B., Fiala, R., Amato, J., Tomaska, L., Sponer, J., Plavec, J., Trantirek, L.
J. Am. Chem. Soc. (2017), 139, 3591-3594, PubMed 28217994 , DOI 10.1021/jacs.6b10786 , Abstract

Experiments performed 10 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker AvanceIII - 700 MHz room temperature inverse (1H-BB) probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	SC11	natural abundance	0.2 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	10 mM

2 2D 1H-1H NOESY

Instrument

Bruker AvanceIII - 850 MHz triple-resonance (1H-13C-15N) cryogenic probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

3 2D 1H-1H TOCSY

Instrument

Bruker AvanceIII - 850 MHz triple-resonance (1H-13C-15N) cryogenic probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

4 2D DQF-COSY

Instrument

Bruker AvanceIII - 700 MHz room temperature inverse (1H-BB) probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

5 2D 1H-1H ROESY

Instrument

Bruker AvanceIII - 700 MHz triple-resonance (1H-13C-15N) cryogenic probe

Sample

#	Name	Isotope labeling	Concentration
1	SC11	natural abundance	0.2 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	10 mM

6 2D 1H-1H ROESY

Instrument

Bruker AvanceIII - 700 MHz triple-resonance (1H-13C-15N) cryogenic probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

7 1D 15N-edited HSQC

Instrument

Varian Uniform NMR System - 600 MHz DD2 console

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.0, Details 0.2 mM partial (12%) 15N, 13C residue-specific labelling SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	SC11	partial (12%) 15N, 13C residue-specific labelling	0.2 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	10 mM

8 1D 13C-edited HSQC

Instrument

Varian Uniform NMR System - 600 MHz DD2 console

Sample

#	Name	Isotope labeling	Concentration
7	SC11	partial (12%) 15N, 13C residue-specific labelling	0.2 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	10 mM

9 diffusion experiment

Instrument

Bruker AvanceIII - 950 MHz triple-resonance (1H-13C-15N) cryogenic probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

10 2D 1H-1H NOESY

Instrument

Bruker AvanceIII - 700 MHz triple-resonance (1H-13C-15N) cryogenic probe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.2 mM / SC11, 10 mM / potassium phosphate, 100 mM / potassium chloride, 100% D2O

#	Name	Isotope labeling	Concentration
4	SC11	natural abundance	0.2 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	10 mM

nullKeywords DNA, G-hairpin G:G base pairs NMR spectroscopy conformational exchange

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Found 1 Document (0.611 seconds)

BMRB: 34053

Sample #1

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Experiments performed 10 experiments Detail

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