BMRB: 34056

Structure of DNA AGCGA-quadruplex adopted by 15-mer oligonucleotide found in regulatory region of the PLEKHG3 human gene with G11 to I11 mutation, d(GCGAGGGAGCIAGGG),VK34_I11

Authors

Kocman, V., Plavec, J.

Assembly

DNA (5'-D(*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*IP*AP*GP*GP*G)-3')

Entity

1. DNA (5'-D(*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*IP*AP*GP*GP*G)-3') (polymer, Thiol state: not present), 15 monomers, 4813.042 × 2 Da Detail

GCGAGGGAGC XAGGG

Total weight

9626.084 Da

Max. entity weight

4813.042 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.0 %, Completeness: 56.2 %, Completeness (bb): 48.0 % Detail

Download

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	56.2 % (73 of 130)	56.2 % (73 of 130)
Suger, PO4	48.0 % (47 of 98)	48.0 % (47 of 98)
Nucleobase	81.3 % (26 of 32)	81.3 % (26 of 32)
Aromatic	78.6 % (22 of 28)	78.6 % (22 of 28)

1. entity 1

GCGAGGGAGC XAGGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6, Details 1 mM DNA (5'-D(*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*IP*AP*GP*GP*G)-3'), 100 mM LiCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*IP*AP*GP*GP*G)-3')	natural abundance		1 mM
2	LiCl	natural abundance		100 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6, Details 1 mM residue specific 15N labeled DNA (5'-D(*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*IP*AP*GP*GP*G)-3'), 100 mM LiCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	DNA (5'-D(*GP*CP*GP*AP*GP*GP*GP*AP*GP*CP*IP*AP*GP*GP*G)-3')	residue specific 15N labeled		1 mM
4	LiCl	natural abundance		100 mM

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Release date

2017-05-21

Citation

Tetrahelical structural family adopted by AGCGA-rich regulatory DNA regions
Kocman, V., Plavec, J.
Nat. Commun. (2017), 8, 15355-15355, PubMed 28513602 , DOI 10.1038/ncomms15355 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 3 experiments Detail