BMRB: 34062

Quadruplex with flipped tetrad formed by a human telomeric sequence

Authors

Dickerhoff, J., Haase, L., Langel, W., Weisz, K.

Assembly

G-quadruplex formed by a human telomeric sequence modified with 2'-fluoro-2'-deoxyriboguanosine

Entity

1. G-quadruplex formed by a human telomeric sequence modified with 2'-fluoro-2'-deoxyriboguanosine (polymer, Thiol state: not present), 24 monomers, 7708.826 Da Detail

TTXGGTTAXG GTTAGGGTTA XGGA

Formula weight

7708.826 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 82.2 %, Completeness (bb): 81.6 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	82.2 % (162 of 197)	82.2 % (162 of 197)
Suger, PO4	81.6 % (120 of 147)	81.6 % (120 of 147)
Nucleobase	84.0 % (42 of 50)	84.0 % (42 of 50)
Aromatic	81.0 % (34 of 42)	81.0 % (34 of 42)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. entity 1

TTXGGTTAXG GTTAGGGTTA XGGA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.59 mM DNA, 10.0 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA	natural abundance		0.59 mM
2	potassium phosphate	natural abundance		10.0 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.59 mM DNA, 10.0 mM potassium phosphate, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	DNA	natural abundance		0.59 mM
4	potassium phosphate	natural abundance		10.0 mM

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Release date

2017-04-10

Citation

Tracing Effects of Fluorine Substitutions on G-Quadruplex Conformational Changes
Dickerhoff, J., Haase, L., Langel, W., Weisz, K.
ACS Chem. Biol. (2017), 12, 1308-1315, PubMed 28318229 , DOI 10.1021/acschembio.6b01096 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. G-quadruplex formed by a human telomeric sequence modified with 2'-fluoro-2'-deoxyriboguanosine, : 1 entities Detail

Experiments performed 5 experiments Detail