BMRB: 34063

Quadruplex with flipped tetrad formed by an artificial sequence

Authors

Dickerhoff, J., Haase, L., Langel, W., Weisz, K.

Assembly

Artificial quadruplex with propeller, diagonal and lateral loop

Entity

1. Artificial quadruplex with propeller, diagonal and lateral loop (polymer, Thiol state: not present), 22 monomers, 7009.454 Da Detail

XGGATXGGAC ACAGGGGACX GG

Formula weight

7009.454 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.4 %, Completeness: 77.0 %, Completeness (bb): 75.2 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	77.0 % (137 of 178)	77.0 % (137 of 178)
Suger, PO4	75.2 % (100 of 133)	75.2 % (100 of 133)
Nucleobase	82.2 % (37 of 45)	82.2 % (37 of 45)
Aromatic	94.7 % (36 of 38)	94.7 % (36 of 38)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. entity 1

XGGATXGGAC ACAGGGGACX GG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.9 mM DNA, 10.0 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA	natural abundance		0.9 mM
2	potassium phosphate	natural abundance		10.0 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.9 mM DNA, 10.0 mM potassium phosphate, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	DNA	natural abundance		0.9 mM
4	potassium phosphate	natural abundance		10.0 mM

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Release date

2017-04-10

Citation

Tracing Effects of Fluorine Substitutions on G-Quadruplex Conformational Changes
Dickerhoff, J., Haase, L., Langel, W., Weisz, K.
ACS Chem. Biol. (2017), 12, 1308-1315, PubMed 28318229 , DOI 10.1021/acschembio.6b01096 , Abstract

Experiments performed 4 experiments Detail