BMRB: 34064

Solution structure and dynamics of the first outer membrane cytochrome from Geobacter sulfurreducens

Authors

Dantas, J.M., Silva, M.A., Morgado, L., Pantoja-Uceda, D., Turner, D.L., Bruix, M., Salgueiro, C.A.

Assembly

Lipoprotein cytochrome c, 1 heme-binding site

Entity

1. Lipoprotein cytochrome c, 1 heme-binding site, entity 1 (polymer), 80 monomers, 8313.364 Da Detail

AGGGELFATH CAGCHPQGGN TVHPEKTLAR ARREANGIRT VRDVAAYIRN PGPGMPAFGE AMIPPADALK IGEYVVASFP

2. Lipoprotein cytochrome c, 1 heme-binding site, entity HEC (non-polymer), 618.503 Da

Total weight

8931.867 Da

Max. entity weight

8313.364 Da

Source organism

Geobacter sulfurreducens PCA

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.4 %, Completeness (bb): 97.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	93.4 % (485 of 519)	93.0 % (412 of 443)	96.1 % (73 of 76)
Backbone	97.9 % (230 of 235)	98.8 % (161 of 163)	95.8 % (69 of 72)
Sidechain	89.8 % (255 of 284)	89.6 % (251 of 280)	100.0 % (4 of 4)
Aromatic	65.5 % (19 of 29)	65.5 % (19 of 29)
Methyl	100.0 % (41 of 41)	100.0 % (41 of 41)

1. entity 1

AGGGELFATH CAGCHPQGGN TVHPEKTLAR ARREANGIRT VRDVAAYIRN PGPGMPAFGE AMIPPADALK IGEYVVASFP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM [U-13C; U-15N] Outer membrane protein, OmcF, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Outer membrane protein, OmcF	[U-13C; U-15N]		2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM Outer membrane protein, OmcF, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Outer membrane protein, OmcF	natural abundance		2 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 2 mM Outer membrane protein, OmcF, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	Outer membrane protein, OmcF	natural abundance		2 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 5MCS, Strand ID: A Detail

---

Release date

2016-12-01

Citation

Solution structure and dynamics of the outer membrane cytochrome OmcF from Geobacter sulfurreducens
Dantas, J.M., Silva, M.A., Pantoja-Uceda, D., Turner, D.L., Bruix, M., Salgueiro, C.A.
Biochim. Biophys. Acta (2017), 1858, 733-741, PubMed 28377068 , DOI 10.1016/j.bbabio.2017.03.007 , Abstract

Related entities 1. Lipoprotein cytochrome c, 1 heme-binding site, entity 1, : 1 : 1 : 1 : 1 : 47 entities Detail

Experiments performed 12 experiments Detail