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BMRB: 34071


34071
5MJX
2'F-ANA/DNA Chimeric TBA Quadruplex structure
Authors
Lietard, J., Abou Assi, H., Gomez-Pinto, I., Gonzalez, C., Somoza, M.M., Damha, M.J.
Assembly
DNA (5'-D(*GP*GP*(FT)P*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3')
Entity
1. DNA (5'-D(*GP*GP*(FT)P*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3') (polymer, Thiol state: not present), 15 monomers, 4824.014 Da Detail

GGXTGGTGTG GTTGG


Formula weight
4824.014 Da
Source organism
synthetic construct
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.3 %, Completeness: 96.9 %, Completeness (bb): 95.9 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All96.9 % (127 of 131)96.9 % (127 of 131)
Suger, PO495.9 % (94 of 98)95.9 % (94 of 98)
Nucleobase100.0 % (33 of 33)100.0 % (33 of 33)
Aromatic100.0 % (28 of 28)100.0 % (28 of 28)
Methyl100.0 % (5 of 5)100.0 % (5 of 5)

1. entity 1

GGXTGGTGTG GTTGG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 279.6 K, pH 7.0, Details 1.0 mg/mL DNA (5'-D(*GP*GP*(FT)P*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3'), 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*GP*GP*(FT)P*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3')natural abundance1.0 mg/mL
2NaPinatural abundance10 mM
3KClnatural abundance25 mM

Release date
2017-01-24
Citation
Mapping the affinity landscape of Thrombin-binding aptamers on 2΄F-ANA/DNA chimeric G-Quadruplex microarrays
Lietard, J., Abou Assi, H., Gomez-Pinto, I., Gonzalez, C., Somoza, M.M., Damha, M.J.
Nucleic Acids Res. (2017), 45, 1619-1632, PubMed 28100695 , DOI 10.1093/nar/gkw1357 ,
Experiments performed 3 experiments Detail
NMR combined restraints 3 contents Detail
Keywords 2'F-ANA/DNA TBA Structure, Structure from MOLMOL, dna