BMRBj - BMREntryMaster

Found 1 Document (0.907 seconds)

BMRB: 34079

5MPG

Solution NMR structure of hnRNP A1 RRM1 in complex with 5'-UUAGGUC-3' RNA

Authors

Barraud, P., Allain, F.

Assembly

Heterogeneous nuclear ribonucleoprotein A1/RNA Complex

Entity

1. Heterogeneous nuclear ribonucleoprotein A1/RNA Complex, entity 1 (polymer, Thiol state: all free), 97 monomers, 10985.12 Da Detail

ASKSESPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN TKRSRGFGFV TYATVEEVDA AMNARPHKVD GRVVEPKRAV SREDSQR

2. Heterogeneous nuclear ribonucleoprotein A1/RNA Complex, entity 2 (polymer, Thiol state: not present), 7 monomers, 2261.311 Da Detail

UUAGGUC

Total weight

13246.432 Da

Max. entity weight

10985.12 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 78.6 %, Completeness (bb): 75.0 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	78.6 % (964 of 1227)	86.7 % (560 of 646)	65.3 % (312 of 478)	89.3 % (92 of 103)
Backbone	75.0 % (487 of 649)	93.2 % (221 of 237)	56.3 % (180 of 320)	93.5 % (86 of 92)
Sidechain	83.1 % (556 of 669)	82.9 % (339 of 409)	84.7 % (211 of 249)	54.5 % (6 of 11)
Aromatic	75.9 % (85 of 112)	87.5 % (49 of 56)	70.0 % (35 of 50)	16.7 % (1 of 6)
Methyl	96.5 % (83 of 86)	95.3 % (41 of 43)	97.7 % (42 of 43)

1. entity 1

ASKSESPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN TKRSRGFGFV TYATVEEVDA AMNARPHKVD GRVVEPKRAV SREDSQR

2. entity 2

UUAGGUC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	hnRNP A1 RRM1	[U-99% 15N]		1.0 mM
2	RNA UUAGGUC	natural abundance		1.0 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	hnRNP A1 RRM1	[U-99% 15N]		1.0 mM
6	RNA UUAGGUC	natural abundance		1.0 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
8	RNA UUAGGUC	natural abundance		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5MPG, Strand ID: A Detail

Release date

2017-11-27

Citation

Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7
Beusch, I., Barraud, P., Moursy, A., Clery, A., Allain, F.H.
Elife (2017), 6, e25736-e25736, PubMed 28650318 , DOI 10.7554/eLife.25736 , Abstract

Related entities 1. Heterogeneous nuclear ribonucleoprotein A1/RNA Complex, entity 1, : 1 : 26 : 361 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	hnRNP A1 RRM1	[U-99% 15N]		1.0 mM
2	RNA UUAGGUC	natural abundance		1.0 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
8	RNA UUAGGUC	natural abundance		1.0 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
8	RNA UUAGGUC	natural abundance		1.0 mM

4 3D HNCA

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

5 3D HNCACB

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

6 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

7 3D HNCO

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

8 3D HCc(CO)NH TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

9 3D hCC(CO)NH TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	hnRNP A1 RRM1	[U-99% 15N]		1.0 mM
2	RNA UUAGGUC	natural abundance		1.0 mM

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

12 3D F1eF3f 13C-filtered/edited NOESY

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
8	RNA UUAGGUC	natural abundance		1.0 mM

13 2D F1fF2f 13C-filtered NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
8	RNA UUAGGUC	natural abundance		1.0 mM

14 2D 1H-1H TOCSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	hnRNP A1 RRM1	[U-99% 15N]		1.0 mM
6	RNA UUAGGUC	natural abundance		1.0 mM

15 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM1, 1.0 mM RNA UUAGGUC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	hnRNP A1 RRM1	[U-99% 15N]		1.0 mM
6	RNA UUAGGUC	natural abundance		1.0 mM

16 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	hnRNP A1 RRM1	[U-99% 13C; U-99% 15N]		1.0 mM
4	RNA UUAGGUC	natural abundance		1.0 mM

nullKeywords RNA, RRM, splicing

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Found 1 Document (0.907 seconds)

BMRB: 34079

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Heterogeneous nuclear ribonucleoprotein A1/RNA Complex, entity 1, : 1 : 26 : 361 entities Detail

Experiments performed 16 experiments Detail

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