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BMRB: 34080

5MPL

hnRNP A1 RRM2 in complex with 5'-UCAGUU-3' RNA

Authors

Barraud, P., Allain, F.H.-T.

Assembly

hnRNP A1 RRM2 in complex with 5'-UCAGUU-3' RNA

Entity

1. Heterogeneous nuclear ribonucleoprotein A1 (polymer, Thiol state: all free), 102 monomers, 11606.90 Da Detail

SQRPGAHLTV KKIFVGGIKE DTEEHHLRDY FEQYGKIEVI EIMTDRGSGK KRGFAFVTFD DHDSVDKIVI QKYHTVNGHN CEVRKALSKQ EMASASSSQR GR

2. RNA UCAGUU (polymer, Thiol state: not present), 6 monomers, 1916.106 Da Detail

UCAGUU

Total weight

13523.006 Da

Max. entity weight

11606.9 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 88.9 %, Completeness: 71.5 %, Completeness (bb): 67.0 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	71.5 % (923 of 1291)	77.7 % (527 of 678)	60.5 % (303 of 501)	83.0 % (93 of 112)
Backbone	67.0 % (453 of 676)	76.6 % (190 of 248)	52.9 % (173 of 327)	89.1 % (90 of 101)
Sidechain	77.4 % (548 of 708)	78.4 % (337 of 430)	77.9 % (208 of 267)	27.3 % (3 of 11)
Aromatic	60.9 % (78 of 128)	73.4 % (47 of 64)	51.7 % (31 of 60)	0.0 % (0 of 4)
Methyl	97.8 % (90 of 92)	97.8 % (45 of 46)	97.8 % (45 of 46)

1. Heterogeneous nuclear ribonucleoprotein A1

SQRPGAHLTV KKIFVGGIKE DTEEHHLRDY FEQYGKIEVI EIMTDRGSGK KRGFAFVTFD DHDSVDKIVI QKYHTVNGHN CEVRKALSKQ EMASASSSQR GR

2. RNA UCAGUU

UCAGUU

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 g/L RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	RNA UCAGUU	natural abundance	1.0 g/L
2	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
9	RNA UCAGUU	natural abundance	1.0 mM
10	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
11	D2O	natural abundance	100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
12	RNA UCAGUU	natural abundance	1.0 mM
13	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
14	D2O	natural abundance	100 %

Chem. Shift Complete2

Sequence coverage: 25.9 %, Completeness: 42.4 %, Completeness (bb): 43.4 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	42.4 % (1094 of 2582)	45.9 % (623 of 1356)	35.7 % (358 of 1002)	50.4 % (113 of 224)
Backbone	43.4 % (587 of 1352)	51.4 % (255 of 496)	33.9 % (222 of 654)	54.5 % (110 of 202)
Sidechain	42.4 % (601 of 1416)	42.8 % (368 of 860)	43.1 % (230 of 534)	13.6 % (3 of 22)
Aromatic	35.2 % (90 of 256)	46.1 % (59 of 128)	25.8 % (31 of 120)	0.0 % (0 of 8)
Methyl	51.6 % (95 of 184)	51.1 % (47 of 92)	52.2 % (48 of 92)

1. Heterogeneous nuclear ribonucleoprotein A1

SQRPGAHLTV KKIFVGGIKE DTEEHHLRDY FEQYGKIEVI EIMTDRGSGK KRGFAFVTFD DHDSVDKIVI QKYHTVNGHN CEVRKALSKQ EMASASSSQR GR

2. RNA UCAGUU

UCAGUU

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 g/L RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	RNA UCAGUU	natural abundance	1.0 g/L
2	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
9	RNA UCAGUU	natural abundance	1.0 mM
10	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
11	D2O	natural abundance	100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
12	RNA UCAGUU	natural abundance	1.0 mM
13	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
14	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5MPL, Strand ID: A Detail

Release date

2016-12-18

Citation

Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7
Beusch, I., Barraud, P., Moursy, A., Clery, A., Allain, F.H.
Elife (2017), 6, e25736-e25736, PubMed 28650318 , DOI 10.7554/eLife.25736 , Abstract

Related entities 1. Heterogeneous nuclear ribonucleoprotein A1, : 1 : 17 : 1 : 11 : 232 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 g/L RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	RNA UCAGUU	natural abundance	1.0 g/L
2	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
12	RNA UCAGUU	natural abundance	1.0 mM
13	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
14	D2O	natural abundance	100 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
12	RNA UCAGUU	natural abundance	1.0 mM
13	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
14	D2O	natural abundance	100 %

4 3D HNCA

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

5 3D HNCACB

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

6 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

7 3D HNCO

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

8 3D HCc(CO)NH TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

9 3D hCC(CO)NH TOCSY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 g/L RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	RNA UCAGUU	natural abundance	1.0 g/L
2	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

12 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	RNA UCAGUU	natural abundance	1.0 mM
6	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

13 3D F1eF3f 13C-filtered/edited NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
12	RNA UCAGUU	natural abundance	1.0 mM
13	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
14	D2O	natural abundance	100 %

14 2D F1fF2f 13C-filtered NOESY

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 13C; U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
12	RNA UCAGUU	natural abundance	1.0 mM
13	hnRNP A1 RRM2	[U-99% 13C; U-99% 15N]	1.0 mM
14	D2O	natural abundance	100 %

15 2D 1H-1H TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
9	RNA UCAGUU	natural abundance	1.0 mM
10	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
11	D2O	natural abundance	100 %

16 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] hnRNP A1 RRM2, 1.0 mM RNA UCAGUU, 100% D2O

#	Name	Isotope labeling	Concentration
9	RNA UCAGUU	natural abundance	1.0 mM
10	hnRNP A1 RRM2	[U-99% 15N]	1.0 mM
11	D2O	natural abundance	100 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_34080_5mpl.nef
Input source #2: Coordindates	5mpl.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---100-------110-------120-------130-------140-------150-------160-------170-------180-------190----
SQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQR
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--
GR
||
GR
--

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

------
UCAGUU
||||||
UCAGUU

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	102	0	0	100.0
B	B	6	0	0	100.0

Content subtype: combined_34080_5mpl.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	902		92.2 (chain: A, length: 102)
2	Assigned chemical shifts	assigned_chemical_shifts_2	Warning	43		100.0 (chain: B, length: 6)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	2667 (1)	noe	90.2 (chain: A, length: 102), 100.0 (chain: B, length: 6)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	46 (1)	hbond	28.4 (chain: A, length: 102), 33.3 (chain: B, length: 6)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	4 (4)	.	66.7 (chain: B, length: 6)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 102, Sequence coverage: 92.2%
- Total number of assignments
  - ¹H chemical shifts: 517
  - ¹³C chemical shifts: 293
  - ¹⁵N chemical shifts: 92
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chemical_shifts_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 43
- Completeness of assignments
  - Entity_assemble_ID: B
- Histogram of normalized assigned chemical shifts

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	625	517	82.7
¹³C chemical shifts	460	293	63.7
¹⁵N chemical shifts	115	92	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	212	186	87.7
¹³C chemical shifts	204	89	43.6
¹⁵N chemical shifts	101	88	87.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	413	331	80.1
¹³C chemical shifts	256	204	79.7
¹⁵N chemical shifts	14	4	28.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	47	97.9
¹³C chemical shifts	48	47	97.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	49	47	95.9
¹³C chemical shifts	49	31	63.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	53	43	81.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	36	32	88.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	11	64.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	3	3	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 102, Sequence coverage: 90.2%
  - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 100.0%
- Total number of restraints: 2665
- Weight of restraints: 1.0 (2665)
- Potential type of restraints: square-well-parabolic (2665)
- Range of distance target values: 2.04, 4.25 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 102, Sequence coverage: 28.4%
    - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 33.3%
  - Total number of restraints: 46
  - Weight of restraints: 1.0 (46)
  - Potential type of restraints: square-well-parabolic (46)
  - Range of distance target values: 2.0, 3.0 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 6, Sequence coverage: 66.7%
- Total number of restraints: 4
- Total number of restraints per polymer type: 4
- Weight of restraints: 1.0 (4)
- Potential type of restraints: square-well-parabolic (4)
- Dihedral angle restraints per residue
  - Chain_ID: B

Keywords RNA, RRM, hnRNP, splicing

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Found 1 Document (0.668 seconds)

BMRB: 34080

Sample #1

Sample #2

Sample #3

Sample #4

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Heterogeneous nuclear ribonucleoprotein A1, : 1 : 17 : 1 : 11 : 232 entities Detail

Experiments performed 16 experiments Detail

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NMR combined restraints 6 contents Detail