BMRB: 34083

G-quadruplex formed within promoters of Plasmodium falciparum B var genes

Authors

Juribasic Kulcsar, M., Plavec, J.

Assembly

UpsB-Q-1, DNA (34-MER)

Entity

1. UpsB-Q-1, DNA (34-MER) (polymer, Thiol state: not present), 34 monomers, 10727.83 Da Detail

CAGGGTTAAG GGTATAACTT TAGGGGTTAG GGTT

Formula weight

10727.83 Da

Source organism

Plasmodium falciparum

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.7 %, Completeness (bb): 86.6 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	85.7 % (275 of 321)	85.7 % (275 of 321)
Suger, PO4	86.6 % (206 of 238)	86.6 % (206 of 238)
Nucleobase	83.1 % (69 of 83)	83.1 % (69 of 83)
Aromatic	85.3 % (58 of 68)	85.3 % (58 of 68)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. entity 1

CAGGGTTAAG GGTATAACTT TAGGGGTTAG GGTT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0, Details 1.6 mM UpsB-Q-1, DNA (34-MER), 150 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	UpsB-Q-1, DNA (34-MER)	natural abundance		1.6 mM
2	potassium chloride	natural abundance		150 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0, Details 1.8 mM UpsB-Q-1, DNA (34-MER), 150 mM potassium chloride, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	UpsB-Q-1, DNA (34-MER)	natural abundance		1.8 mM
4	potassium chloride	natural abundance		150 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0, Details 1 mM 8% 13C, 8% 15N UpsB-Q-1, DNA (34-MER), 150 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	UpsB-Q-1, DNA (34-MER)	8% 13C, 8% 15N		1 mM
6	potassium chloride	natural abundance		150 mM

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Release date

2018-02-19

Citation

Stabilizing interactions in long-loop G-quadruplex formed within promoters of Plasmodium falciparum B var genes
Juribasic Kulcsar, M., Gabelica, V., Plavec, J.

Experiments performed 5 experiments Detail