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BMRB: 34094

5N14

NMR structure calculation of a composite Cys2His2 type zinc finger protein containing a non-peptide (or oligourea) helical domain

Authors

Venkateshaiah M, V.K., Salgado, G.

Assembly

Protein (chimeric oligourea-peptide zinc finger)

Entity

1. Protein (chimeric oligourea-peptide zinc finger), entity 1 (polymer), 25 monomers, 3249.804 Da Detail

PFACDICGRK FARSXXXXXX XXRKD

2. Protein (chimeric oligourea-peptide zinc finger), entity ZN (non-polymer), 65.409 Da
3. Protein (chimeric oligourea-peptide zinc finger), entity HOH (water), 18.015 Da

Total weight

3315.213 Da

Max. entity weight

3249.804 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 68.0 %, Completeness: 75.1 %, Completeness (bb): 79.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.1 % (151 of 201)	95.4 % (103 of 108)	42.9 % (33 of 77)	93.8 % (15 of 16)
Backbone	79.0 % (79 of 100)	100.0 % (34 of 34)	60.0 % (30 of 50)	93.8 % (15 of 16)
Sidechain	73.5 % (86 of 117)	93.2 % (69 of 74)	39.5 % (17 of 43)
Aromatic	40.0 % (8 of 20)	80.0 % (8 of 10)	0.0 % (0 of 10)
Methyl	100.0 % (8 of 8)	100.0 % (4 of 4)	100.0 % (4 of 4)

1. entity 1

PFACDICGRK FARSXXXXXX XXRKD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

Protein Blocks Logo

Calculated from 10 models in PDB: 5N14, Strand ID: A Detail

Release date

2017-04-03

Citation

NMR structure calculation of a composite Cys2His2 type zinc finger protein containing a non-peptide (or oligourea) helical domain
Venkateshaiah Machohally, V.K., Salgado, G., Lombardo, C., Mergny, J.L., Guichard, G.

Related entities 1. Protein (chimeric oligourea-peptide zinc finger), entity 1, : 1 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 950 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

2 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 950 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

3 2D 1H-1H TOCSY

Instrument

Bruker AvanceIII - 950 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

4 2D 1H-1H NOESY

Instrument

Bruker AvanceIII - 950 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

5 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 800 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

6 2D 1H-1H TOCSY

Instrument

Bruker AvanceIII - 800 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

7 2D 1H-1H COSY

Instrument

Bruker AvanceIII - 950 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

8 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 800 MHz Equipped with CryoProbe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

9 2D 1H-1H NOESY

Instrument

Bruker AvanceIII - 800 MHz Equipped with CryoProbe

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 3.2 mM CL112, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CL112	natural abundance		3.2 (±0.2) mM

nullKeywords CHIMERIC, OULIGOUREAS, TRANSCRIPTION-DNA COMPLEX, ZINC FINGER