BMRB: 34105

Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH.

Authors

Roret, T., Tsan, P.

Assembly

Dehydroascorbate reductase family protein

Entity

1. Dehydroascorbate reductase family protein, entity 1 (polymer, Thiol state: free and other bound), 218 monomers, 24325.85 Da Detail

MALEICVKAA VGAPNILGDC PFCQRVLLSL EEKKIPYKSH LINLGDKPQW FLEISPEGKV PVVKIDDKWV ADSDVIVGIL EEKNPEPPLA TPPEFASVGS KIFPSFVKFL KSKDPNDGTE QALLEELKAL DGHLKVHGPF IAGEKITAVD LSLAPKLYHL EVALGHFKNW PIPDNLTHVL NYIKLLFSRE SFKKTRAAEE HVIAGWEPKV NAHHHHHH

2. Dehydroascorbate reductase family protein, entity GSH (non-polymer), 307.323 Da

Total weight

24633.172 Da

Max. entity weight

24325.85 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS20:SG	2:GSH1:SG2

Source organism

Populus trichocarpa

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 87.2 %, Completeness: 31.2 %, Completeness (bb): 64.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	31.2 % (491 of 1572)	22.2 % (301 of 1357)	88.4 % (190 of 215)
Backbone	64.1 % (404 of 630)	50.0 % (215 of 430)	94.5 % (189 of 200)
Sidechain	9.2 % (87 of 942)	9.3 % (86 of 927)	6.7 % (1 of 15)
Aromatic	0.0 % (0 of 116)	0.0 % (0 of 112)	0.0 % (0 of 4)
Methyl	11.9 % (16 of 134)	11.9 % (16 of 134)

1. entity 1

MALEICVKAA VGAPNILGDC PFCQRVLLSL EEKKIPYKSH LINLGDKPQW FLEISPEGKV PVVKIDDKWV ADSDVIVGIL EEKNPEPPLA TPPEFASVGS KIFPSFVKFL KSKDPNDGTE QALLEELKAL DGHLKVHGPF IAGEKITAVD LSLAPKLYHL EVALGHFKNW PIPDNLTHVL NYIKLLFSRE SFKKTRAAEE HVIAGWEPKV NAHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0, Details 0.37 mM [U-100% 15N] Dehydroascorbate reductase, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Dehydroascorbate reductase	[U-100% 15N]		0.37 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0, Details 0.37 mM [U-100% 15N] Dehydroascorbate reductase, 74.0 mM GLUTATHIONE, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Dehydroascorbate reductase	[U-100% 15N]		0.37 mM
3	GLUTATHIONE	natural abundance		74.0 mM

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Chem. Shift Complete2

Sequence coverage: 88.5 %, Completeness: 31.6 %, Completeness (bb): 64.6 % Detail

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• BMRB/XML (partial)
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Polymer type: polypeptide(L)

	Total	1H	15N
All	31.6 % (994 of 3144)	22.6 % (613 of 2714)	88.6 % (381 of 430)
Backbone	64.6 % (814 of 1260)	50.5 % (434 of 860)	95.0 % (380 of 400)
Sidechain	9.6 % (180 of 1884)	9.7 % (179 of 1854)	3.3 % (1 of 30)
Aromatic	0.0 % (0 of 232)	0.0 % (0 of 224)	0.0 % (0 of 8)
Methyl	14.9 % (40 of 268)	14.9 % (40 of 268)

1. entity 1

MALEICVKAA VGAPNILGDC PFCQRVLLSL EEKKIPYKSH LINLGDKPQW FLEISPEGKV PVVKIDDKWV ADSDVIVGIL EEKNPEPPLA TPPEFASVGS KIFPSFVKFL KSKDPNDGTE QALLEELKAL DGHLKVHGPF IAGEKITAVD LSLAPKLYHL EVALGHFKNW PIPDNLTHVL NYIKLLFSRE SFKKTRAAEE HVIAGWEPKV NAHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0, Details 0.37 mM [U-100% 15N] Dehydroascorbate reductase, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Dehydroascorbate reductase	[U-100% 15N]		0.37 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0, Details 0.37 mM [U-100% 15N] Dehydroascorbate reductase, 74.0 mM GLUTATHIONE, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Dehydroascorbate reductase	[U-100% 15N]		0.37 mM
3	GLUTATHIONE	natural abundance		74.0 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 5N9U, Strand ID: A Detail

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Release date

2017-03-06

Citation

Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa
Lallement, P.A., Roret, T., Tsan, P., Gualberto, J.M., Girardet, J.M., Didierjean, C., Rouhier, N., Hecker, A.
Biochem. J. (2016), 473, 717-731, PubMed 26699905 , DOI 10.1042/BJ20151147 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Dehydroascorbate reductase family protein, entity 1, : 1 : 1 : 1 : 154 entities Detail

Experiments performed 1 experiments Detail