BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.9 % (480 of 586)	88.6 % (264 of 298)	70.4 % (162 of 230)	93.1 % (54 of 58)
Backbone	82.4 % (262 of 318)	95.5 % (105 of 110)	69.0 % (107 of 155)	94.3 % (50 of 53)
Sidechain	84.2 % (267 of 317)	84.6 % (159 of 188)	83.9 % (104 of 124)	80.0 % (4 of 5)
Aromatic	50.0 % (7 of 14)	71.4 % (5 of 7)	28.6 % (2 of 7)
Methyl	88.4 % (76 of 86)	97.7 % (42 of 43)	79.1 % (34 of 43)

1. entity 1

VSDISALTVG QALKVKAGQN AMDATVLEIT KDGVRVQLNS GMSLIVRAEH LVF

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20 mM sodium phosphate, 100 mM sodium chloride, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	0.0 ~ 0.0 mM
2	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
3	TCEP	natural abundance	1 (±0.1) mM
4	sodium chloride	natural abundance	100 (±5.0) mM
5	sodium phosphate	natural abundance	20 (±1.0) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20 mM sodium chloride, 100 mM sodium phosphate, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	internal	direct	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5NBB, Strand ID: A Detail

Release date

2017-05-01

Citation

Structure of the Escherichia coli ProQ RNA-binding protein
Gonzalez, G.M., Hardwick, S.W., Maslen, S.L., Skehel, J.M., Holmqvist, E., Vogel, J., Bateman, A., Luisi, B.F., Broadhurst, R.W.
RNA (2017), 23, 696-711, PubMed 28193673 , DOI 10.1261/rna.060343.116 , Abstract

Related entities 1. RNA chaperone ProQ, : 10 : 40 entities Detail

Interaction partners 1. RNA chaperone ProQ, : 43 interactors Detail

More details for 43 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20 mM sodium phosphate, 100 mM sodium chloride, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	0.0 ~ 0.0 mM
2	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
3	TCEP	natural abundance	1 (±0.1) mM
4	sodium chloride	natural abundance	100 (±5.0) mM
5	sodium phosphate	natural abundance	20 (±1.0) mM

2 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20 mM sodium chloride, 100 mM sodium phosphate, 1 mM TCEP, 0.0025 % 3,3,3-trimethylsilylpropionate, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

3 3D HNCA

Instrument

Bruker DRX - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

4 3D HN(CO)CA

Instrument

Bruker DRX - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

5 3D 1H-15N TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	0.0 ~ 0.0 mM
2	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
3	TCEP	natural abundance	1 (±0.1) mM
4	sodium chloride	natural abundance	100 (±5.0) mM
5	sodium phosphate	natural abundance	20 (±1.0) mM

6 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

7 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

8 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	entity_1	natural abundance	0.0 ~ 0.0 mM
7	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
8	TCEP	natural abundance	1 (±0.1) mM
9	sodium chloride	natural abundance	20 (±1.0) mM
10	sodium phosphate	natural abundance	100 (±5.0) mM

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	0.0 ~ 0.0 mM
2	3,3,3-trimethylsilylpropionate	natural abundance	0.0025 (±5.0E-4) %
3	TCEP	natural abundance	1 (±0.1) mM
4	sodium chloride	natural abundance	100 (±5.0) mM
5	sodium phosphate	natural abundance	20 (±1.0) mM

nullKeywords FinO, ProQ, RNA chaperone, chaperone

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.87 seconds)

BMRB: 34111

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA chaperone ProQ, : 10 : 40 entities Detail

Interaction partners 1. RNA chaperone ProQ, : 43 interactors Detail

Experiments performed 10 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample