BMRBj - BMREntryMaster

Found 1 Document (5.915 seconds)

BMRB: 34115

5NF8

Solution structure of detergent-solubilized Rcf1, a yeast mitochondrial inner membrane protein involved in respiratory Complex III/IV supercomplex formation

Authors

Zhou, S., Pettersson, P., Sjoholm, J., Sjostrand, D., Hogbom, M., Brzezinski, P., Maler, L., Adelroth, P.

Assembly

Respiratory supercomplex factor 1, mitochondrial

Entity

1. Respiratory supercomplex factor 1, mitochondrial (polymer, Thiol state: all free), 174 monomers, 20347.15 × 2 Da Detail

MSRMPSSFDV TERDLDDMTF GERIIYHCKK QPLVPIGCLL TTGAVILAAQ NVRLGNKWKA QYYFRWRVGL QAATLVALVA GSFIYGTSGK ELKAKEEQLK EKAKMREKLW IQELERREEE TEARRKRAEL ARMKTLENEE EIKNLEKELS DLENKLGKKE FRVPGSHHHH HHHH

Total weight

40694.3 Da

Max. entity weight

20347.15 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 78.3 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.3 % (1660 of 2119)	77.7 % (869 of 1119)	78.3 % (639 of 816)	82.6 % (152 of 184)
Backbone	87.6 % (908 of 1036)	85.3 % (302 of 354)	88.9 % (455 of 512)	88.8 % (151 of 170)
Sidechain	72.2 % (900 of 1247)	74.1 % (567 of 765)	70.9 % (332 of 468)	7.1 % (1 of 14)
Aromatic	16.2 % (25 of 154)	29.9 % (23 of 77)	2.7 % (2 of 74)	0.0 % (0 of 3)
Methyl	89.3 % (159 of 178)	88.8 % (79 of 89)	89.9 % (80 of 89)

1. entity 1

MSRMPSSFDV TERDLDDMTF GERIIYHCKK QPLVPIGCLL TTGAVILAAQ NVRLGNKWKA QYYFRWRVGL QAATLVALVA GSFIYGTSGK ELKAKEEQLK EKAKMREKLW IQELERREEE TEARRKRAEL ARMKTLENEE EIKNLEKELS DLENKLGKKE FRVPGSHHHH HHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-15N] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	rcf1	[U-15N]		0.4 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-13C; U-15N; U-2H] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	rcf1	[U-13C; U-15N; U-2H]		0.4 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-13C; U-15N] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	rcf1	[U-13C; U-15N]		0.4 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-13C; U-15N; 50% U-2H] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	rcf1	[U-13C; U-15N; 50% U-2H]		0.4 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-15N; U-2H; 99%-1HD, 13CD-IL; 99%-1HG, 13CG-V] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	rcf1	[U-15N; U-2H; 99%-1HD, 13CD-IL; 99%-1HG, 13CG-V]		0.4 mM

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-50% 13C; U-50% 15N; U-50% 12C; U-50% 14N] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
6	rcf1	[U-50% 13C; U-50% 15N; U-50% 12C; U-50% 14N]		0.4 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	40.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	115.4 ppm	internal	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	40.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	115.4 ppm	internal	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	40.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	115.4 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	40.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	115.4 ppm	internal	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 5NF8, Strand ID: A, B Detail

Release date

2018-02-19

Citation

Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation
Zhou, S., Pettersson, P., Huang, J., Sjoholm, J., Sjostrand, D., Pomes, R., Hogbom, M., Brzezinski, P., Maler, L., Adelroth, P.
Proc. Natl. Acad. Sci. U. S. A. (2018), 115, 3048-3053, PubMed 29507228 , DOI 10.1073/pnas.1712061115 , Abstract

Related entities 1. Respiratory supercomplex factor 1, mitochondrial, : 1 : 2 : 13 entities Detail

Interaction partners 1. Respiratory supercomplex factor 1, mitochondrial, : 5 interactors Detail

More details for 5 interactors identified by 2 citations

Experiments performed 22 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-15N] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	rcf1	[U-15N]		0.4 mM

2 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 323 K, pH 5.0, Details 0.4 mM [U-15N] rcf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	rcf1	[U-15N]		0.4 mM

3 3D HNCACB