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BMRB: 34123

5NPA

Solution structure of Drosophila melanogaster Loquacious dsRBD2

Authors

Tants, J., Fesser, S., Kern, T., Stehle, R., Geerlof, A., Wunderlich, C., Boettcher, R., Kunzelmann, S., Lange, O., Kreutz, C., Foerstemann, K., Sattler, M.

Assembly

Loquacious

Entity

1. Loquacious (polymer), 72 monomers, 8519.926 Da Detail

DKTVGIGWLQ EMCMQRRWPP PSYETETEVG LPHERLFTIA CSILNYREMG KGKSKKIAKR LAAHRMWMRL QE

Formula weight

8519.926 Da

Source organism

Drosophila melanogaster

Exptl. method

Refine. method

Rasrec algorithm

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.3 %, Completeness: 59.9 %, Completeness (bb): 67.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.9 % (532 of 888)	66.9 % (316 of 472)	45.5 % (155 of 341)	81.3 % (61 of 75)
Backbone	67.9 % (288 of 424)	88.3 % (128 of 145)	46.9 % (99 of 211)	89.7 % (61 of 68)
Sidechain	55.2 % (293 of 531)	57.5 % (188 of 327)	53.3 % (105 of 197)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 70)	0.0 % (0 of 35)	0.0 % (0 of 32)	0.0 % (0 of 3)
Methyl	85.9 % (55 of 64)	84.4 % (27 of 32)	87.5 % (28 of 32)

1. entity 1

DKTVGIGWLQ EMCMQRRWPP PSYETETEVG LPHERLFTIA CSILNYREMG KGKSKKIAKR LAAHRMWMRL QE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5NPA, Strand ID: A Detail

Release date

2017-10-26

Citation

Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference
Tants, J., Fesser, S., Kern, T., Stehle, R., Geerlof, A., Wunderlich, C., Juen, M., Hartlmuller, C., Boettcher, R., Kunzelmann, S., Lange, O., Kreutz, C., Foerstemann, K., Sattler, M.
Nucleic Acids Res. (2017), 45, 12536-12550, PubMed 29040648 , DOI 10.1093/nar/gkx886 , Abstract

Related entities 1. Loquacious, : 1 : 166 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

2 3D HNCA

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

3 3D HNCACB

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

4 3D HNCACO

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

5 3D HNCO

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

6 3D HN(COCA)CB

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

7 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

8 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

9 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD2	[U-13C; U-15N]		2 (±0.2) g/L
2	NaCl	natural abundance		500 mM
3	PO4	natural abundance		20 mM

nullKeywords RNA binding protein, RNA interference, dsRBD, siRNA