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BMRB: 34124

5NPG

Solution structure of Drosophila melanogaster Loquacious dsRBD1

Authors

Tants, J., Fesser, S., Kern, T., Stehle, R., Geerlof, A., Wunderlich, C., Hartlmuller, C., Boettcher, R., Kunzelmann, S., Lange, O., Kreutz, C., Foerstemann, K., Sattler, M.

Assembly

Loquacious, isoform B

Entity

1. Loquacious, isoform B (polymer, Thiol state: not present), 83 monomers, 9090.447 Da Detail

NGLAMKTPVS ILQELLSRRG ITPGYELVQI EGAIHEPTFR FRVSFKDKDT PFTAMGAGRS KKEAKHAAAR ALIDKLIGAQ LPE

Formula weight

9090.447 Da

Source organism

Drosophila melanogaster

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 73.6 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.6 % (712 of 968)	74.8 % (379 of 507)	67.8 % (257 of 379)	92.7 % (76 of 82)
Backbone	82.2 % (401 of 488)	94.6 % (159 of 168)	68.6 % (166 of 242)	97.4 % (76 of 78)
Sidechain	69.1 % (384 of 556)	64.9 % (220 of 339)	77.0 % (164 of 213)	0.0 % (0 of 4)
Aromatic	28.6 % (16 of 56)	53.6 % (15 of 28)	3.6 % (1 of 28)
Methyl	83.7 % (82 of 98)	83.7 % (41 of 49)	83.7 % (41 of 49)

1. entity 1

NGLAMKTPVS ILQELLSRRG ITPGYELVQI EGAIHEPTFR FRVSFKDKDT PFTAMGAGRS KKEAKHAAAR ALIDKLIGAQ LPE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

LACS Plot; CA

Referencing offset: -0.52 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.52 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5NPG, Strand ID: A Detail

Release date

2017-09-26

Citation

Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference
Tants, J., Fesser, S., Kern, T., Stehle, R., Geerlof, A., Wunderlich, C., Boettcher, R., Kunzelmann, S., Lange, O., Kreutz, C., Foerstemann, K., Sattler, M.
Nucleic Acids Res. (2017), 45, 12536-12550, PubMed 29040648 , DOI 10.1093/nar/gkx886 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 34123 released on 2017-10-26
Title Solution structure of Drosophila melanogaster Loquacious dsRBD2

Related entities 1. Loquacious, isoform B, : 1 : 14 : 211 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

2 3D HNCACB

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

3 3D HNCA

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

4 3D HNCO

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

5 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

6 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

7 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 101325 Pa, Temperature 298 K, pH 6.5, Details 2 g/L [U-13C; U-15N] dsRBD1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBD1	[U-13C; U-15N]		2 g/L
2	NaCl	natural abundance		500 mM
3	PO4 buffer	natural abundance		20 mM

nullKeywords RNA BINDING PROTEIN, RNA interference, dsRBD, siRNA