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Found 1 Document (0.952 seconds)

BMRB: 34127

5NR6

NMR structure and 1H, 13C and 15N signal assignments for Dictyostelium discoidans MATB protein S71A mutant

Authors

Neuhaus, D., Hedgethorne, K., Yang, J.

Assembly

MatB protein

Entity

1. MatB protein (polymer, Thiol state: not present), 113 monomers, 12636.13 Da Detail

GSHMASMDQL DEIIEQIQKE AINSNVVLKN PRVPTQKTGE LSEEQKKIVA DYISEVGLNN LNATELSKRL NITVDKAKTY IKNSNRMGRT NNFKTIKMFE DDVSSASAQP NLP

Formula weight

12636.13 Da

Source organism

Dictyostelium discoideum

Exptl. method

solution NMR

Refine. method

simulated annealingsimulated annealingmolecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.3 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.3 % (1114 of 1321)	89.6 % (623 of 695)	73.5 % (367 of 499)	97.6 % (124 of 127)
Backbone	84.0 % (563 of 670)	99.1 % (224 of 226)	69.3 % (232 of 335)	98.2 % (107 of 109)
Sidechain	86.4 % (657 of 760)	85.1 % (399 of 469)	88.3 % (241 of 273)	94.4 % (17 of 18)
Aromatic	65.0 % (26 of 40)	90.0 % (18 of 20)	40.0 % (8 of 20)
Methyl	97.6 % (121 of 124)	98.4 % (61 of 62)	96.8 % (60 of 62)

1. entity 1

GSHMASMDQL DEIIEQIQKE AINSNVVLKN PRVPTQKTGE LSEEQKKIVA DYISEVGLNN LNATELSKRL NITVDKAKTY IKNSNRMGRT NNFKTIKMFE DDVSSASAQP NLP

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 274 K, pH 6.0, Details 600 uM [U-15N] MATB, 25 mM sodium phosphate, 100 mM sodium chloride, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	EDTA	natural abundance	50 uM
2	MATB	[U-15N]	600 (±100.0) uM
3	sodium chloride	natural abundance	100 mM
4	sodium phosphate	natural abundance	25 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 274 K, pH 6.0, Details 600 uM [U-13C; U-15N] MATB, 25 mM sodium phosphate, 100 mM sodium chloride, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 5NR6, Strand ID: A Detail

Release date

2017-05-07

Citation

Homeodomain-like DNA binding proteins control the haploid-to-diploid transition in Dictyostelium
Hedgethorne, K., Eustermann, S., Yang, J.C., Ogden, T., Neuhaus, D., Bloomfield, G.
Sci. Adv. (2017), 3, e1602937-e1602937, PubMed 28879231 , DOI 10.1126/sciadv.1602937 , Abstract

Related entities 1. MatB protein, : 1 : 10 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 274 K, pH 6.0, Details 600 uM [U-15N] MATB, 25 mM sodium phosphate, 100 mM sodium chloride, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	EDTA	natural abundance	50 uM
2	MATB	[U-15N]	600 (±100.0) uM
3	sodium chloride	natural abundance	100 mM
4	sodium phosphate	natural abundance	25 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 274 K, pH 6.0, Details 600 uM [U-13C; U-15N] MATB, 25 mM sodium phosphate, 100 mM sodium chloride, 50 uM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

4 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

5 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

6 3D HNHAHB

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

7 3D HBHA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

8 3D [1H-13C-1H] HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

9 3D [13C-13C-1H] HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

10 3D [1H-13C-1H] HCCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

11 3D 1H-15N NOESY (mix. time 150ms)

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

12 3D 1H-13C NOESY aliphatic (mix. time 150ms)

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

13 3D 1H-13C NOESY aromatic (mix. time 150ms)

Instrument

Bruker DMX - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
5	EDTA	natural abundance	50 uM
6	MATB	[U-13C; U-15N]	600 (±100.0) uM
7	sodium chloride	natural abundance	100 mM
8	sodium phosphate	natural abundance	25 mM

nullKeywords DNA binding protein, Homeobox, Mating type determination factor

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Found 1 Document (0.952 seconds)

BMRB: 34127

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MatB protein, : 1 : 10 entities Detail

Experiments performed 13 experiments Detail

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