BMRB: 34137

M2 G-quadruplex 10 wt% PEG8000

Authors

Trajkovski, M., Plavec, J., Endoh, T., Tateishi-Karimata, H., Sugimoto, N.

Assembly

DNA (5'-D(*TP*AP*GP*GP*GP*AP*CP*GP*GP*GP*CP*GP*GP*GP*CP*AP*GP*GP*GP*T)-3')

Entity

1. DNA (5'-D(*TP*AP*GP*GP*GP*AP*CP*GP*GP*GP*CP*GP*GP*GP*CP*AP*GP*GP*GP*T)-3') (polymer, Thiol state: not present), 20 monomers, 6384.035 Da Detail

TAGGGACGGG CGGGCAGGGT

Formula weight

6384.035 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 100.0 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	95.7 % (180 of 188)	95.7 % (180 of 188)
Suger, PO4	100.0 % (140 of 140)	100.0 % (140 of 140)
Nucleobase	83.3 % (40 of 48)	83.3 % (40 of 48)
Aromatic	95.0 % (38 of 40)	95.0 % (38 of 40)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. entity 1

TAGGGACGGG CGGGCAGGGT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.0, Details 0.2 mM 0 M2 10PEG8000, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	M2 10PEG8000	natural abundance		0.2 mM
2	potassium chloride	natural abundance		100 mM
3	potassium phosphate	natural abundance		20 mM

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Release date

2018-04-02

Citation

Pursuing origins of (poly)ethylene glycol-induced G-quadruplex structural modulations
Trajkovski, M., Endoh, T., Tateishi-Karimata, H., Ohyama, T., Tanaka, S., Plavec, J., Sugimoto, N.
Nucleic Acids Res. (2018), 46, 4301-4315, PubMed 29648656 , DOI 10.1093/nar/gky250 , Abstract

Experiments performed 2 experiments Detail