BMRB: 34145

G-quadruplex of Human papillomavirus type 52

Authors

Marusic, M., Plavec, J.

Assembly

G-quadruplex of Human papillomavirus type 52

Entity

1. G-quadruplex of Human papillomavirus type 52 (polymer, Thiol state: not present), 23 monomers, 7339.654 Da Detail

GGGTAGGGCA GGGGACACAG GGT

Formula weight

7339.654 Da

Source organism

human papillomavirus 52

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.3 %, Completeness (bb): 99.4 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	95.3 % (205 of 215)	95.3 % (205 of 215)
Suger, PO4	99.4 % (160 of 161)	99.4 % (160 of 161)
Nucleobase	83.3 % (45 of 54)	83.3 % (45 of 54)
Aromatic	93.5 % (43 of 46)	93.5 % (43 of 46)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. entity 1

GGGTAGGGCA GGGGACACAG GGT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.05), Details 1.2 mM DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*CP*AP*GP*GP*GP*GP*AP*CP*AP*CP*AP*GP*GP*GP*T)-3'), 10 mM potassium phosphate, 40 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		1.2 (±0.1) mM
2	potassium phosphate	natural abundance		10 (±0.5) mM
3	potassium chloride	natural abundance		40 (±0.5) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.05), Details 1.2 mM DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*CP*AP*GP*GP*GP*GP*AP*CP*AP*CP*AP*GP*GP*GP*T)-3'), 10 mM potassium phosphate, 40 mM potassium chloride, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	entity_1	natural abundance		1.2 (±0.1) mM
5	potassium phosphate	natural abundance		10 (±0.5) mM
6	potassium chloride	natural abundance		40 (±0.5) mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 6.8 (±0.05), Details 0.9 mM 15N site-specifically labelled at 5% DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*CP*AP*GP*GP*GP*GP*AP*CP*AP*CP*AP*GP*GP*GP*T)-3'), 10 mM potassium phosphate, 40 mM potassium chloride, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	entity_1	15N site-specifically labelled at 5%		0.9 (±0.1) mM
8	potassium phosphate	natural abundance		10 (±0.5) mM
9	potassium chloride	natural abundance		40 (±0.5) mM

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Release date

2018-06-05

Citation

Towards Understanding of Polymorphism of the G-rich Region of Human Papillomavirus Type 52
Marusic, M., Plavec, J.
Molecules (2019), 24, E1294-E1294, PubMed 30987050 , DOI 10.3390/molecules24071294 , Abstract

Experiments performed 6 experiments Detail