BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	75.2 % (671 of 892)	73.3 % (344 of 469)	75.5 % (262 of 347)	85.5 % (65 of 76)
Backbone	86.7 % (371 of 428)	88.7 % (126 of 142)	83.9 % (183 of 218)	91.2 % (62 of 68)
Sidechain	67.4 % (361 of 536)	66.7 % (218 of 327)	69.7 % (140 of 201)	37.5 % (3 of 8)
Aromatic	51.9 % (28 of 54)	74.1 % (20 of 27)	26.9 % (7 of 26)	100.0 % (1 of 1)
Methyl	80.7 % (71 of 88)	81.8 % (36 of 44)	79.5 % (35 of 44)

1. RST domain from the Radical-Induced Cell Death1

TTRPKSPWMP FPTLFAAISH KVAENDMLLI NADYQQLRDK KMTRAEFVRK LRVIVGDDLL RSTITTLQNQ PKS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-13C; U-15N] RCD1-RST, 200 uM DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 150 uM [U-15N] RCD1-RST, 150 uM DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	DREB2A	natural abundance	150 uM
7	DSS	natural abundance	0.7 mM
8	RCD1-RST	[U-15N]	150 uM
9	sodium azide	natural abundance	0.2 mg/mL
10	NaCl	natural abundance	100 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-13C; U-15N] RCD1-RST, 200 uM [U-13C; U-15N] DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 300 uM [U-15N] RCD1-RST, 900 uM DREB2A, 50 mg/mL C12E5, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
16	C12E5	natural abundance	50 mg/mL
17	DREB2A	natural abundance	900 uM
18	DSS	natural abundance	0.7 mM
19	RCD1-RST	[U-15N]	300 uM
20	sodium azide	natural abundance	0.2 mg/mL
21	NaCl	natural abundance	100 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 5OAO, Strand ID: A Detail

Release date

2017-07-16

Citation

Connecting the αα-hubs: same fold, disordered ligands, new functions
Bugge, K., Staby, L., Kemplen, K.R., O'Shea, C., Bendsen, S.K., Jensen, M.K., Olsen, J.G., Skriver, K., Kragelund, B.B.
Cell Commun. Signal. (2021), 19, 2-2, PubMed 33407551 , DOI 10.1186/s12964-020-00686-8 , Abstract

Related entities 1. Inactive poly [ADP-ribose] polymerase RCD1, : 1 : 3 : 30 entities Detail

Interaction partners 1. Inactive poly [ADP-ribose] polymerase RCD1, : 30 interactors Detail

More details for 30 interactors identified by 4 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-13C; U-15N] RCD1-RST, 200 uM DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

2 3D HNCACB

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

4 3D HNCA

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

5 3D HN(CO)CA

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

6 3D 1H-15N TOCSY

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

7 3D HNHA

Instrument

Varian INOVA - 750 MHz Room temperature probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 200 uM [U-13C; U-15N] RCD1-RST, 200 uM [U-13C; U-15N] DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

8 3D HNCO

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 750 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

10 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

11 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

12 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

13 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

14 2D 1H-15N HSQC

Instrument

Bruker Avance - 750 MHz Cryo probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 150 uM [U-15N] RCD1-RST, 150 uM DREB2A, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	DREB2A	natural abundance	150 uM
7	DSS	natural abundance	0.7 mM
8	RCD1-RST	[U-15N]	150 uM
9	sodium azide	natural abundance	0.2 mg/mL
10	NaCl	natural abundance	100 mM

15 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
1	DREB2A	natural abundance	200 uM
2	DSS	natural abundance	0.7 mM
3	RCD1-RST	[U-13C; U-15N]	200 uM
4	sodium azide	natural abundance	0.2 mg/mL
5	NaCl	natural abundance	100 mM

16 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

17 2D 1H-15N IPAP-HSQC

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 300 uM [U-15N] RCD1-RST, 900 uM DREB2A, 50 mg/mL C12E5, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
16	C12E5	natural abundance	50 mg/mL
17	DREB2A	natural abundance	900 uM
18	DSS	natural abundance	0.7 mM
19	RCD1-RST	[U-15N]	300 uM
20	sodium azide	natural abundance	0.2 mg/mL
21	NaCl	natural abundance	100 mM

18 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Cryo probe

Sample

#	Name	Isotope labeling	Concentration
11	DREB2A	[U-13C; U-15N]	200 uM
12	DSS	natural abundance	0.7 mM
13	RCD1-RST	[U-13C; U-15N]	200 uM
14	sodium azide	natural abundance	0.2 mg/mL
15	NaCl	natural abundance	100 mM

19 2D 1H-15N IPAP-HSQC

Instrument

Varian INOVA - 800 MHz Room temperature probe

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 300 uM [U-15N] RCD1-RST, 900 uM DREB2A, 50 mg/mL C12E5, 0.2 mg/mL sodium azide, 0.7 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
16	C12E5	natural abundance	50 mg/mL
17	DREB2A	natural abundance	900 uM
18	DSS	natural abundance	0.7 mM
19	RCD1-RST	[U-15N]	300 uM
20	sodium azide	natural abundance	0.2 mg/mL
21	NaCl	natural abundance	100 mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_34151_5oao.nef
Input source #2: Coordindates	5oao.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

500-----510-------520-------530-------540-------550-------560-------570--
TTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQPKS
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
TTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQPKS
--------10--------20--------30--------40--------50--------60--------70---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	73	0	0	100.0

Content subtype: combined_34151_5oao.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	608		83.6 (chain: A, length: 73)
1	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	1467 (1)	noe	80.8 (chain: A, length: 73)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	110 (110)	.	79.5 (chain: A, length: 73)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_2	OK	51 (51)	.	69.9 (chain: A, length: 73)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 73, Sequence coverage: 83.6%
- Total number of assignments
  - ¹H chemical shifts: 303
  - ¹⁵N chemical shifts: 60
  - ¹³C chemical shifts: 245
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	469	303	64.6
¹⁵N chemical shifts	82	60	73.2
¹³C chemical shifts	347	245	70.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	142	119	83.8
¹⁵N chemical shifts	68	59	86.8
¹³C chemical shifts	146	114	78.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	327	184	56.3
¹⁵N chemical shifts	14	1	7.1
¹³C chemical shifts	201	131	65.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	39	83.0
¹³C chemical shifts	47	38	80.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	13	48.1
¹⁵N chemical shifts	1	1	100.0
¹³C chemical shifts	26	7	26.9

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_4
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 73, Sequence coverage: 80.8%
- Total number of restraints: 1433
- Weight of restraints: 1.0 (1433)
- Potential type of restraints: square-well-parabolic (1433)
- Range of distance target values: 2.04, 5.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 73, Sequence coverage: 79.5%
- Total number of restraints: 110
- Total number of restraints per polymer type: 110
- Weight of restraints: 1.0 (110)
- Potential type of restraints: square-well-parabolic (110)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_2

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 73, Sequence coverage: 69.9%

500-----510-------520-------530-------540-------550-------560-------570--
TTRPKSPWMPFPTLFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQPKS
  |         ||||| | |  |||| ||||||||||||||||| ||| |||||||||||||| |||||   
..R.........TLFAA.S.K..ENDM.LINADYQQLRDKKMTRA.FVR.LRVIVGDDLLRSTI.TLQNQ   
500-----510-------520-------530-------540-------550-------560---------

Total number of restraints: 51
Potential type of restraints: undefined (51)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords L-glove, PROTEIN BINDING, alpha-alpha hairpin, hub, transcription factor binding

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Found 1 Document (0.792 seconds)

BMRB: 34151

Sample #1

Sample #2

Sample #3

Sample #4

Related entities 1. Inactive poly [ADP-ribose] polymerase RCD1, : 1 : 3 : 30 entities Detail

Interaction partners 1. Inactive poly [ADP-ribose] polymerase RCD1, : 30 interactors Detail

Experiments performed 19 experiments Detail

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NMR combined restraints 5 contents Detail