BMRB: 34161

Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant

Authors

Vuister, G.W., Bokhovchuk, F.M., Bate, N., Kovalevskaya, N., Goult, B.T., Spronk, C.A.E.M.

Assembly

Calmodulin-1, Transient receptor potential cation channel subfamily V member 5

Entity

1. Calmodulin-1, Transient receptor potential cation channel subfamily V member 5, entity 1 (polymer, Thiol state: not present), 150 monomers, 16922.52 Da Detail

SMADQLTEEQ IAEFKEAFSL FDKDGDGTIT TKQLGTVMRS LGQNPTEAEL QDMINEVDAD GNGTIDFPQF LTMMARKMKD TDSEEEIREA FRVFDKDGNG YISAAELRHV MTNLGEKLTD EEVDEMIREA DIDGDGQVNY EEFVQMMTAK

2. Calmodulin-1, Transient receptor potential cation channel subfamily V member 5, entity 2 (polymer, Thiol state: not present), 73 monomers, 7713.118 Da Detail

GADKEDDQEH PSEKQPSGAE SGTLARASLA LPTSSLSRTA SQSSSHRGWE ILRQNTLGHL NLGLNLSEGD GEE

3. Calmodulin-1, Transient receptor potential cation channel subfamily V member 5, entity CA (non-polymer), 40.078 × 2 Da

Total weight

24715.793 Da

Max. entity weight

16922.52 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealingmolecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.5 %, Completeness: 66.3 %, Completeness (bb): 64.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	66.3 % (1636 of 2468)	72.5 % (931 of 1284)	55.3 % (522 of 944)	76.2 % (183 of 240)
Backbone	64.3 % (854 of 1328)	74.6 % (343 of 460)	52.3 % (340 of 650)	78.4 % (171 of 218)
Sidechain	69.6 % (935 of 1344)	71.4 % (588 of 824)	67.3 % (335 of 498)	54.5 % (12 of 22)
Aromatic	53.2 % (66 of 124)	72.6 % (45 of 62)	32.8 % (20 of 61)	100.0 % (1 of 1)
Methyl	76.7 % (158 of 206)	78.6 % (81 of 103)	74.8 % (77 of 103)

1. entity 1

SMADQLTEEQ IAEFKEAFSL FDKDGDGTIT TKQLGTVMRS LGQNPTEAEL QDMINEVDAD GNGTIDFPQF LTMMARKMKD TDSEEEIREA FRVFDKDGNG YISAAELRHV MTNLGEKLTD EEVDEMIREA DIDGDGQVNY EEFVQMMTAK

2. entity 2

GADKEDDQEH PSEKQPSGAE SGTLARASLA LPTSSLSRTA SQSSSHRGWE ILRQNTLGHL NLGLNLSEGD GEE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 (±0.1) atm, Temperature 308 (±0.1) K, pH 7.4 (±0.1), Details 220 uM 13C/15N Calmodulin, 220 uM hTRPV5(655-725), 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]		220 (±0.05) uM
2	hTRPV5(655-725)	natural abundance		220 (±0.05) uM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 (±0.1) atm, Temperature 308 (±0.1) K, pH 7.4 (±0.1), Details 380 uM [U-99% 15N] Calmodulin, 380 uM [U-99% 13C; U-99% 15N] hTRPV5(655-725), 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
3	Calmodulin	[U-99% 15N]		380 (±0.05) uM
4	hTRPV5(655-725)	[U-99% 13C; U-99% 15N]		380 (±0.05) uM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 5OEO, Strand ID: A, C Detail

---

Release date

2018-04-15

Citation

The Structural Basis of Calcium Dependent Inactivation of the Transient Receptor Potential Vanilloid 5 Channel
Bokhovchuk, F.M., Bate, N., Kovalevskaya, N.V., Goult, B.T., Spronk, C.A.E.M., Vuister, G.W.
Biochemistry (2018), 57, 2623-2635, PubMed 29584409 , DOI 10.1021/acs.biochem.7b01287 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Calmodulin-1, Transient receptor potential cation channel subfamily V member 5, entity 1, : 99 entities Detail

Related entities 2. Calmodulin-1, Transient receptor potential cation channel subfamily V member 5, entity 2, : 1 : 10 entities Detail

Interaction partners 2. Calmodulin-1, Transient receptor potential cation channel subfamily V member 5, entity 2, : 3 interactors Detail

Experiments performed 4 experiments Detail