BMRB: 34163

Structure of DNA-binding HU protein from micoplasma Spiroplasma melliferum

Authors

Altukhov, D.A., Talyzina, A.A., Agapova, Y.K., Vlaskina, A.V., Korzhenevskiy, D.A., Bocharov, E.V., Rakitina, T.V., Timofeev, V.I.

Assembly

DNA-binding protein

Entity

1. DNA-binding protein (polymer, Thiol state: not present), 95 monomers, 10271.63 × 2 Da Detail

GHMSKKELAA QIAEKFTDVL SKTHAEEITN FVFDHIKKAL VAGKEVSIAG FGKFAVTERA ARDGRNPSTG ETIKIPASKS AKFKAGKQLK TDLNN

Total weight

20543.26 Da

Max. entity weight

10271.63 Da

Source organism

Spiroplasma melliferum KC3

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.3 %, Completeness: 33.6 %, Completeness (bb): 61.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	33.6 % (369 of 1098)	25.9 % (148 of 572)	32.8 % (140 of 427)	81.8 % (81 of 99)
Backbone	61.0 % (345 of 566)	67.2 % (131 of 195)	47.8 % (133 of 278)	87.1 % (81 of 93)
Sidechain	11.3 % (70 of 620)	4.5 % (17 of 377)	22.4 % (53 of 237)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 72)	0.0 % (0 of 36)	0.0 % (0 of 36)
Methyl	15.4 % (16 of 104)	9.6 % (5 of 52)	21.2 % (11 of 52)

1. DNA-binding protein

GHMSKKELAA QIAEKFTDVL SKTHAEEITN FVFDHIKKAL VAGKEVSIAG FGKFAVTERA ARDGRNPSTG ETIKIPASKS AKFKAGKQLK TDLNN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.7, Details 5 % [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	na		0.0 ~ 0.0 mM
2	D2O	[U-2H]		5 %

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Protein Blocks Logo

Calculated from 15 models in PDB: 5OGU, Strand ID: A, B Detail

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Release date

2017-07-23

Citation

Structural plasticity and thermal stability of the histone-like protein from Spiroplasma melliferum are due to phenylalanine insertions into the conservative scaffold
Timofeev, V.I., Altukhov, D.A., Talyzina, A.A., Agapova, Y.K., Vlaskina, A.V., Korzhenevskiy, D.A., Kleymenov, S.Y., Bocharov, E.V., Rakitina, T.V.
J. Biomol. Struct. Dyn. (2018), 36, 1-13, PubMed 29283021 , DOI 10.1080/07391102.2017.1417162 , Abstract

Related entities 1. DNA-binding protein, : 1 : 1 : 161 entities Detail

Experiments performed 2 experiments Detail