BMRB: 34168

G-quadruplex structure of DNA oligonucleotide containing GGGGCC repeats linked to ALS and FTD

Authors

Brcic, J., Plavec, J.

Assembly

DNA (5'-D(*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*(BGM)P*G)-3')

Entity

1. DNA (5'-D(*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*(BGM)P*G)-3') (polymer, Thiol state: not present), 22 monomers, 7099.293 Da Detail

GGGGCCGGGG CCGGGGCCGG XG

Formula weight

7099.293 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 76.1 %, Completeness (bb): 76.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	76.1 % (153 of 201)	76.1 % (153 of 201)
Suger, PO4	76.9 % (113 of 147)	76.9 % (113 of 147)
Nucleobase	74.1 % (40 of 54)	74.1 % (40 of 54)
Aromatic	95.2 % (40 of 42)	95.2 % (40 of 42)

1. entity 1

GGGGCCGGGG CCGGGGCCGG XG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.8, Details 1.0 mM DNA (5'-D(*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*(BGM)P*G)-3'), 30 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		1.0 mM
2	potassium chloride	natural abundance		30 mM
3	potassium phosphate	natural abundance		20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 bar, Temperature 298 K, pH 5.8, Details 0.4 mM DNA (5'-D(*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*(BGM)P*G)-3'), 30 mM potassium chloride, 20 mM potassium phosphate, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	entity_1	natural abundance		0.4 mM
5	potassium chloride	natural abundance		30 mM
6	potassium phosphate	natural abundance		20 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.8, Details 0.3 mM one guanine in the sequence labeled 5% 15N and 13 C DNA (5'-D(*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*(BGM)P*G)-3'), \ 30 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
7	entity_1	one guanine in the sequence labeled 5% 15N and 13 C		0.3 mM
8	potassium chloride	natural abundance		30 mM
9	potassium phosphate	natural abundance		20 mM

Hide sample codition

Release date

2018-08-19

Citation

ALS and FTD linked GGGGCC-repeat containing DNA oligonucleotide folds into two distinct G-quadruplexes
Brcic, J., Plavec, J.
Biochim. Biophys. Acta. Gen. Subj. (2017), 1861, 1237-1245, PubMed 27856299 , DOI 10.1016/j.bbagen.2016.11.018 , Abstract

Related entities 1. DNA (5'-D(*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*GP*GP*CP*CP*GP*GP*(BGM)P*G)-3'), : 2 entities Detail

Experiments performed 8 experiments Detail