BMRB: 34171

NMR derived model of the 5'-splice site of SMN2 in complex with the 5'-end of U1 snRNA

Authors

Campagne, S., Clery, A., Allain, F.H.

Assembly

RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3')

Entity

1. RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3'), entity 1 (polymer, Thiol state: not present), 11 monomers, 3491.046 Da Detail

AUACXXACCU G

2. RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3'), entity 2 (polymer, Thiol state: not present), 11 monomers, 3610.134 Da Detail

GGAGUAAGUC U

Total weight

7101.1797 Da

Max. entity weight

3610.134 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 92.5 %, Completeness (bb): 96.8 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C
All	92.5 % (285 of 308)	86.7 % (150 of 173)	100.0 % (135 of 135)
Suger, PO4	96.8 % (213 of 220)	94.2 % (113 of 120)	100.0 % (100 of 100)
Nucleobase	81.8 % (72 of 88)	69.8 % (37 of 53)	100.0 % (35 of 35)
Aromatic	90.0 % (72 of 80)	82.2 % (37 of 45)	100.0 % (35 of 35)

1. entity 1

AUACXXACCU G

2. entity 2

GGAGUAAGUC U

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 bar, Temperature 283 K, pH 5.5, Details 1 mM RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), 1 mM RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3'), 10 mM deuterated MES, 50 mM sodium chloride, 100% D2O.

#	Name	Isotope labeling	Type	Concentration
1	MES	deuterated		10 mM
2	RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3')	natural abundance		1 mM
3	RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3')	natural abundance		1 mM
4	sodium chloride	natural abundance		50 mM

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Release date

2017-08-16

Citation

Binding to SMN2 pre-mRNA-protein complex elicits specificity for small molecule splicing modifiers
Sivaramakrishnan, M., McCarthy, K.D., Campagne, S., Huber, S., Meier, S., Augustin, A., Heckel, T., Meistermann, H., Hug, M.N., Birrer, P., Moursy, A., Khawaja, S., Schmucki, R., Berntenis, N., Giroud, N., Golling, S., Tzouros, M., Banfai, B., Duran-Pacheco, G., Lamerz, J., Hsiu Liu, Y., Luebbers, T., Ratni, H., Ebeling, M., Clery, A., Paushkin, S., Krainer, A.R., Allain, F.H., Metzger, F.
Nat. Commun. (2017), 8, 1476-1476, PubMed 29133793 , DOI 10.1038/s41467-017-01559-4 , Abstract

Experiments performed 4 experiments Detail